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Circular RNA TFO as antiviral therapy against feline infectious peritonitis virus

Feline Infectious Peritonitis Virus (FIPV) is a feline coronavirus (FCoV) which causes a fatal immune-mediated disease called Feline Infectious Peritonitis (FIP) in cats. Antiviral treatments using Ribavirin and interferons have been used to treat the disease symptomatically. However, these treatmen...

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Main Authors: Choong, Oi Kuan, Harun, Mohammad Syamsul Reza, Tejo, Bimo Ario, Omar, Abdul Rahman
Format: Conference or Workshop Item
Language:English
Published: 2012
Online Access:http://psasir.upm.edu.my/id/eprint/50205/1/2-2.pdf
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spelling oai:psasir.upm.edu.my:50205 http://psasir.upm.edu.my/id/eprint/50205/ Circular RNA TFO as antiviral therapy against feline infectious peritonitis virus Choong, Oi Kuan Harun, Mohammad Syamsul Reza Tejo, Bimo Ario Omar, Abdul Rahman Feline Infectious Peritonitis Virus (FIPV) is a feline coronavirus (FCoV) which causes a fatal immune-mediated disease called Feline Infectious Peritonitis (FIP) in cats. Antiviral treatments using Ribavirin and interferons have been used to treat the disease symptomatically. However, these treatments are ineffective. Furthermore, vaccine is ineffective to control FIP in cats. RNA based molecular therapy such as siRNA, miRNA and RNA aptamers have been studied against various infectious diseases and cancers. Little is known on the potential of Triple Helix-Forming Oligonucleotide (TFO) RNA as anti-viral therapy. Hence, TFO based RNA therapy is chosen as a molecular therapy to inhibit FIPV replication. Five sets of FIPV specific circular TFO RNA (TFO1 to TFO5) targeting the selected regions of virulent FIPV were designed and tested in vitro. The antiviral effect of circular TFOs was determined using cell culture and qRT-PCR assays. The results showed that all the circular TFO RNA, except for TFO2 RNA, significantly inhibited FIPV replication by reducing the viral RNA copy numbers up to 100,000 fold compared to control cells. In addition, circular TFO RNA with antiviral activity showed strong binding kinetics with a Kd of ~ 0.1μM. Hence, all the circular TFO except for TFO2 demonstrated antiviral effect on FIPV. This study describes the potential use of TFO as antiviral agent against coronavirus, FIPV in cats. 2012 Conference or Workshop Item PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/50205/1/2-2.pdf Choong, Oi Kuan and Harun, Mohammad Syamsul Reza and Tejo, Bimo Ario and Omar, Abdul Rahman (2012) Circular RNA TFO as antiviral therapy against feline infectious peritonitis virus. In: International Conference on One Health and 24th VAM Congress 2012, 21-23 Sept. 2012, Marriott Putrajaya. (pp. 293-295).
institution UPM IR
collection UPM IR
language English
description Feline Infectious Peritonitis Virus (FIPV) is a feline coronavirus (FCoV) which causes a fatal immune-mediated disease called Feline Infectious Peritonitis (FIP) in cats. Antiviral treatments using Ribavirin and interferons have been used to treat the disease symptomatically. However, these treatments are ineffective. Furthermore, vaccine is ineffective to control FIP in cats. RNA based molecular therapy such as siRNA, miRNA and RNA aptamers have been studied against various infectious diseases and cancers. Little is known on the potential of Triple Helix-Forming Oligonucleotide (TFO) RNA as anti-viral therapy. Hence, TFO based RNA therapy is chosen as a molecular therapy to inhibit FIPV replication. Five sets of FIPV specific circular TFO RNA (TFO1 to TFO5) targeting the selected regions of virulent FIPV were designed and tested in vitro. The antiviral effect of circular TFOs was determined using cell culture and qRT-PCR assays. The results showed that all the circular TFO RNA, except for TFO2 RNA, significantly inhibited FIPV replication by reducing the viral RNA copy numbers up to 100,000 fold compared to control cells. In addition, circular TFO RNA with antiviral activity showed strong binding kinetics with a Kd of ~ 0.1μM. Hence, all the circular TFO except for TFO2 demonstrated antiviral effect on FIPV. This study describes the potential use of TFO as antiviral agent against coronavirus, FIPV in cats.
format Conference or Workshop Item
author Choong, Oi Kuan
Harun, Mohammad Syamsul Reza
Tejo, Bimo Ario
Omar, Abdul Rahman
spellingShingle Choong, Oi Kuan
Harun, Mohammad Syamsul Reza
Tejo, Bimo Ario
Omar, Abdul Rahman
Circular RNA TFO as antiviral therapy against feline infectious peritonitis virus
author_facet Choong, Oi Kuan
Harun, Mohammad Syamsul Reza
Tejo, Bimo Ario
Omar, Abdul Rahman
author_sort Choong, Oi Kuan
title Circular RNA TFO as antiviral therapy against feline infectious peritonitis virus
title_short Circular RNA TFO as antiviral therapy against feline infectious peritonitis virus
title_full Circular RNA TFO as antiviral therapy against feline infectious peritonitis virus
title_fullStr Circular RNA TFO as antiviral therapy against feline infectious peritonitis virus
title_full_unstemmed Circular RNA TFO as antiviral therapy against feline infectious peritonitis virus
title_sort circular rna tfo as antiviral therapy against feline infectious peritonitis virus
publishDate 2012
url http://psasir.upm.edu.my/id/eprint/50205/1/2-2.pdf
_version_ 1819297028398645248
score 13.4562235

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