Affichage MARC: Erythrocyte glutathione peroxidase activity analysis: manual colourimetric method versus commercial ELISA kit

Erythrocyte glutathione peroxidase activity analysis: manual colourimetric method versus commercial ELISA kit

This study was conducted to determine erythrocyte glutathione peroxidase (GSH-Px) activity, in pregnant Boer does supplemented daily with and without dried Morinda citrifolia fruit, analysed using two different methods; a manual colourimetric method (direct method) and a commercial GSH-Px ELISA kit...

Description complète

Enregistré dans:

Détails bibliographiques
Auteurs principaux:	Alsharif, A. H. K. H., Hamzah, Hazilawati, Shaari, Mohd Rosly, Mohamed Mustapha, Noordin, Sithambaram, Shanmugavelu, Haron, Abd Wahid, K., Musaddim, A. M., Nabil
Format:	Conference or Workshop Item
Langue:	English
Publié:	2012
Accès en ligne:	http://psasir.upm.edu.my/id/eprint/50234/1/2-14.pdf
Tags:	Ajouter un tag Pas de tags, Soyez le premier à ajouter un tag!

id	oai:psasir.upm.edu.my:50234
record_format	eprints
spelling	oai:psasir.upm.edu.my:50234 http://psasir.upm.edu.my/id/eprint/50234/ Erythrocyte glutathione peroxidase activity analysis: manual colourimetric method versus commercial ELISA kit Alsharif, A. H. K. H. Hamzah, Hazilawati Shaari, Mohd Rosly Mohamed Mustapha, Noordin Sithambaram, Shanmugavelu Haron, Abd Wahid K., Musaddim A. M., Nabil This study was conducted to determine erythrocyte glutathione peroxidase (GSH-Px) activity, in pregnant Boer does supplemented daily with and without dried Morinda citrifolia fruit, analysed using two different methods; a manual colourimetric method (direct method) and a commercial GSH-Px ELISA kit. The does were supplemented with M. citrifolia fruit started from third trimester of pregnancy until 2.5 months post partum. Results showed that GSH-Px analysed using the direct method produced insignificant differences between the groups throughout the experimental period, while the commercial ELISA kit produced some significant differences between the groups during the experiment. These indicate that erythrocyte GSH-Px activity is more stable analysed using the commercial kit and hence provides more accurate results compared to the direct method. 2012 Conference or Workshop Item PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/50234/1/2-14.pdf Alsharif, A. H. K. H. and Hamzah, Hazilawati and Shaari, Mohd Rosly and Mohamed Mustapha, Noordin and Sithambaram, Shanmugavelu and Haron, Abd Wahid and K., Musaddim and A. M., Nabil (2012) Erythrocyte glutathione peroxidase activity analysis: manual colourimetric method versus commercial ELISA kit. In: International Conference on One Health and 24th VAM Congress 2012, 21-23 Sept. 2012, Marriott Putrajaya. (pp. 379-381).
institution	UPM IR
collection	UPM IR
language	English
description	This study was conducted to determine erythrocyte glutathione peroxidase (GSH-Px) activity, in pregnant Boer does supplemented daily with and without dried Morinda citrifolia fruit, analysed using two different methods; a manual colourimetric method (direct method) and a commercial GSH-Px ELISA kit. The does were supplemented with M. citrifolia fruit started from third trimester of pregnancy until 2.5 months post partum. Results showed that GSH-Px analysed using the direct method produced insignificant differences between the groups throughout the experimental period, while the commercial ELISA kit produced some significant differences between the groups during the experiment. These indicate that erythrocyte GSH-Px activity is more stable analysed using the commercial kit and hence provides more accurate results compared to the direct method.
format	Conference or Workshop Item
author	Alsharif, A. H. K. H. Hamzah, Hazilawati Shaari, Mohd Rosly Mohamed Mustapha, Noordin Sithambaram, Shanmugavelu Haron, Abd Wahid K., Musaddim A. M., Nabil
spellingShingle	Alsharif, A. H. K. H. Hamzah, Hazilawati Shaari, Mohd Rosly Mohamed Mustapha, Noordin Sithambaram, Shanmugavelu Haron, Abd Wahid K., Musaddim A. M., Nabil Erythrocyte glutathione peroxidase activity analysis: manual colourimetric method versus commercial ELISA kit
author_facet	Alsharif, A. H. K. H. Hamzah, Hazilawati Shaari, Mohd Rosly Mohamed Mustapha, Noordin Sithambaram, Shanmugavelu Haron, Abd Wahid K., Musaddim A. M., Nabil
author_sort	Alsharif, A. H. K. H.
title	Erythrocyte glutathione peroxidase activity analysis: manual colourimetric method versus commercial ELISA kit
title_short	Erythrocyte glutathione peroxidase activity analysis: manual colourimetric method versus commercial ELISA kit
title_full	Erythrocyte glutathione peroxidase activity analysis: manual colourimetric method versus commercial ELISA kit
title_fullStr	Erythrocyte glutathione peroxidase activity analysis: manual colourimetric method versus commercial ELISA kit
title_full_unstemmed	Erythrocyte glutathione peroxidase activity analysis: manual colourimetric method versus commercial ELISA kit
title_sort	erythrocyte glutathione peroxidase activity analysis: manual colourimetric method versus commercial elisa kit
publishDate	2012
url	http://psasir.upm.edu.my/id/eprint/50234/1/2-14.pdf
_version_	1819297031527596032
score	13.4562235

Erythrocyte glutathione peroxidase activity analysis: manual colourimetric method versus commercial ELISA kit

Documents similaires