Cover Image

Supplementation of antioxidant BHT to different bull semen extenders enhances semen quality after chilling

The effects of adding various concentrations of antioxidant, butylated hydroxytoluene (BHT) on chilled bull semen for 72 h at 4°C in Bioxcell® (BX), Tris egg-yolk- (TEY) and citrate egg-yolk- (CEY) based semen extenders were studied. Twenty-two ejaculates collected from three bulls, each extended us...

Full description

Saved in:
Bibliographic Details
Main Authors: Mada, Khumran Armiya'u, Degu, Nurhusien Yimer, Yusoff, Rosnina, Omar, Mohamed Ariff, Haron, Abd Wahid, Kaka, Asmatullah, Ebrahimi, Mahdi, Aliyu, Abdulrasheed Bello
Format: Article
Language:English
Published: Universiti Putra Malaysia Press 2017
Online Access:http://psasir.upm.edu.my/id/eprint/51626/1/10-JTAS-0881-2016-5thProof.pdf
Tags: Add Tag
No Tags, Be the first to tag this record!
id oai:psasir.upm.edu.my:51626
record_format eprints
spelling oai:psasir.upm.edu.my:51626 http://psasir.upm.edu.my/id/eprint/51626/ Supplementation of antioxidant BHT to different bull semen extenders enhances semen quality after chilling Mada, Khumran Armiya'u Degu, Nurhusien Yimer Yusoff, Rosnina Omar, Mohamed Ariff Haron, Abd Wahid Kaka, Asmatullah Ebrahimi, Mahdi Aliyu, Abdulrasheed Bello The effects of adding various concentrations of antioxidant, butylated hydroxytoluene (BHT) on chilled bull semen for 72 h at 4°C in Bioxcell® (BX), Tris egg-yolk- (TEY) and citrate egg-yolk- (CEY) based semen extenders were studied. Twenty-two ejaculates collected from three bulls, each extended using three extenders with BHT at 0 (control), 0.5, 1.0, 1.5, 2.0 and 3.0 mM/mL, were evaluated for sperm quality parameters. Extended semen was packaged into 0.25 mL straws containing 20 x 106 spermatozoa and chilled for 72 h. Four random straws each from the control and treatment groups were warmed at 37°C, pooled and evaluated using a computer-assisted semen analyser (IVOS Hamilton Thorne Biosciences) for general and progressive motilities, and for morphology, viability and acrosome damage using eosin-nigrosin stain under phase-contrast microscope. Results showed that sperm morphology, viability and protection of acrosome damage were significantly improved (p<0.05) at BHT concentrations of 0.5 in BX and 1.0 mM/mL in TEY and CEY compared to the controls. The BHT also showed a potential positive effect on progressive motility at 0.5 mM/mL in BX and 1.0 mM/mL in TEY and CEY. High concentrations of BHT (2.0 and 3.0 mM/mL), however, produced deteriorative effects on the sperm parameters in all the extenders. In conclusion, BHT when added at 0.5 mM/mL in BX and 1.0 mM/mL concentration to TEY and CEY extenders improved the quality parameters of bull chilled semen. Universiti Putra Malaysia Press 2017 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/51626/1/10-JTAS-0881-2016-5thProof.pdf Mada, Khumran Armiya'u and Degu, Nurhusien Yimer and Yusoff, Rosnina and Omar, Mohamed Ariff and Haron, Abd Wahid and Kaka, Asmatullah and Ebrahimi, Mahdi and Aliyu, Abdulrasheed Bello (2017) Supplementation of antioxidant BHT to different bull semen extenders enhances semen quality after chilling. Pertanika Journal of Tropical Agricultural Science, 40 (1). pp. 131-142. ISSN 1511-3701; ESSN: 2231-8542 http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2040%20(1)%20Feb.%202017/10-JTAS-0881-2016-5thProof.pdf
institution UPM IR
collection UPM IR
language English
description The effects of adding various concentrations of antioxidant, butylated hydroxytoluene (BHT) on chilled bull semen for 72 h at 4°C in Bioxcell® (BX), Tris egg-yolk- (TEY) and citrate egg-yolk- (CEY) based semen extenders were studied. Twenty-two ejaculates collected from three bulls, each extended using three extenders with BHT at 0 (control), 0.5, 1.0, 1.5, 2.0 and 3.0 mM/mL, were evaluated for sperm quality parameters. Extended semen was packaged into 0.25 mL straws containing 20 x 106 spermatozoa and chilled for 72 h. Four random straws each from the control and treatment groups were warmed at 37°C, pooled and evaluated using a computer-assisted semen analyser (IVOS Hamilton Thorne Biosciences) for general and progressive motilities, and for morphology, viability and acrosome damage using eosin-nigrosin stain under phase-contrast microscope. Results showed that sperm morphology, viability and protection of acrosome damage were significantly improved (p<0.05) at BHT concentrations of 0.5 in BX and 1.0 mM/mL in TEY and CEY compared to the controls. The BHT also showed a potential positive effect on progressive motility at 0.5 mM/mL in BX and 1.0 mM/mL in TEY and CEY. High concentrations of BHT (2.0 and 3.0 mM/mL), however, produced deteriorative effects on the sperm parameters in all the extenders. In conclusion, BHT when added at 0.5 mM/mL in BX and 1.0 mM/mL concentration to TEY and CEY extenders improved the quality parameters of bull chilled semen.
format Article
author Mada, Khumran Armiya'u
Degu, Nurhusien Yimer
Yusoff, Rosnina
Omar, Mohamed Ariff
Haron, Abd Wahid
Kaka, Asmatullah
Ebrahimi, Mahdi
Aliyu, Abdulrasheed Bello
spellingShingle Mada, Khumran Armiya'u
Degu, Nurhusien Yimer
Yusoff, Rosnina
Omar, Mohamed Ariff
Haron, Abd Wahid
Kaka, Asmatullah
Ebrahimi, Mahdi
Aliyu, Abdulrasheed Bello
Supplementation of antioxidant BHT to different bull semen extenders enhances semen quality after chilling
author_facet Mada, Khumran Armiya'u
Degu, Nurhusien Yimer
Yusoff, Rosnina
Omar, Mohamed Ariff
Haron, Abd Wahid
Kaka, Asmatullah
Ebrahimi, Mahdi
Aliyu, Abdulrasheed Bello
author_sort Mada, Khumran Armiya'u
title Supplementation of antioxidant BHT to different bull semen extenders enhances semen quality after chilling
title_short Supplementation of antioxidant BHT to different bull semen extenders enhances semen quality after chilling
title_full Supplementation of antioxidant BHT to different bull semen extenders enhances semen quality after chilling
title_fullStr Supplementation of antioxidant BHT to different bull semen extenders enhances semen quality after chilling
title_full_unstemmed Supplementation of antioxidant BHT to different bull semen extenders enhances semen quality after chilling
title_sort supplementation of antioxidant bht to different bull semen extenders enhances semen quality after chilling
publisher Universiti Putra Malaysia Press
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/51626/1/10-JTAS-0881-2016-5thProof.pdf
_version_ 1819297177430654976
score 13.4562235

Similar Items