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Reduction of MTT to purple formazan by vitamin E isomers in the absence of cells

The yellow tetrazolium salt 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) is widely used to determine cell viability in cell proliferation and cytotoxic assays. MTT is reduced by metabolically active cells to form an insoluble purple formazan product that is quantifiable by sp...

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Main Authors: Lim, Su Wen, Loh, Hwei San, Ting, Kang Nee, Bradshaw, Tracey Dawn, Allaudin, Zeenathul Nazariah
פורמט: Article
שפה:English
יצא לאור: Penerbit Universiti Sains Malaysia 2015
גישה מקוונת:http://psasir.upm.edu.my/id/eprint/51955/1/Reduction%20of%20MTT%20to%20purple%20formazan%20by%20vitamin%20E%20isomers%20in%20the%20absence%20of%20cells.pdf
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spelling oai:psasir.upm.edu.my:51955 http://psasir.upm.edu.my/id/eprint/51955/ Reduction of MTT to purple formazan by vitamin E isomers in the absence of cells Lim, Su Wen Loh, Hwei San Ting, Kang Nee Bradshaw, Tracey Dawn Allaudin, Zeenathul Nazariah The yellow tetrazolium salt 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) is widely used to determine cell viability in cell proliferation and cytotoxic assays. MTT is reduced by metabolically active cells to form an insoluble purple formazan product that is quantifiable by spectrophotometry. It is the most common and direct assay for cell viability. However, in this present study, we demonstrated that the vitamin E isomers α-β-γ-δ-tocotrienols and α-tocopherol were able to reduce MTT into a formazan product, despite the absence of living cells. For comparison, a second method for determining cell viability, which is the neutral red uptake assay, was used in parallel with the MTT assay. The results showed that neutral red did not interact with the vitamin E isomers. Our findings suggest that the MTT assay is not suitable for studying the proliferative effects of vitamin E isomers on cell growth. Penerbit Universiti Sains Malaysia 2015 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/51955/1/Reduction%20of%20MTT%20to%20purple%20formazan%20by%20vitamin%20E%20isomers%20in%20the%20absence%20of%20cells.pdf Lim, Su Wen and Loh, Hwei San and Ting, Kang Nee and Bradshaw, Tracey Dawn and Allaudin, Zeenathul Nazariah (2015) Reduction of MTT to purple formazan by vitamin E isomers in the absence of cells. Tropical Life Sciences Research, 26 (1). pp. 111-120. ISSN 1985-3718; ESSN: 2180-4249 http://www.tlsr.usm.my/TLSRvol26no1.html
institution UPM IR
collection UPM IR
language English
description The yellow tetrazolium salt 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) is widely used to determine cell viability in cell proliferation and cytotoxic assays. MTT is reduced by metabolically active cells to form an insoluble purple formazan product that is quantifiable by spectrophotometry. It is the most common and direct assay for cell viability. However, in this present study, we demonstrated that the vitamin E isomers α-β-γ-δ-tocotrienols and α-tocopherol were able to reduce MTT into a formazan product, despite the absence of living cells. For comparison, a second method for determining cell viability, which is the neutral red uptake assay, was used in parallel with the MTT assay. The results showed that neutral red did not interact with the vitamin E isomers. Our findings suggest that the MTT assay is not suitable for studying the proliferative effects of vitamin E isomers on cell growth.
format Article
author Lim, Su Wen
Loh, Hwei San
Ting, Kang Nee
Bradshaw, Tracey Dawn
Allaudin, Zeenathul Nazariah
spellingShingle Lim, Su Wen
Loh, Hwei San
Ting, Kang Nee
Bradshaw, Tracey Dawn
Allaudin, Zeenathul Nazariah
Reduction of MTT to purple formazan by vitamin E isomers in the absence of cells
author_facet Lim, Su Wen
Loh, Hwei San
Ting, Kang Nee
Bradshaw, Tracey Dawn
Allaudin, Zeenathul Nazariah
author_sort Lim, Su Wen
title Reduction of MTT to purple formazan by vitamin E isomers in the absence of cells
title_short Reduction of MTT to purple formazan by vitamin E isomers in the absence of cells
title_full Reduction of MTT to purple formazan by vitamin E isomers in the absence of cells
title_fullStr Reduction of MTT to purple formazan by vitamin E isomers in the absence of cells
title_full_unstemmed Reduction of MTT to purple formazan by vitamin E isomers in the absence of cells
title_sort reduction of mtt to purple formazan by vitamin e isomers in the absence of cells
publisher Penerbit Universiti Sains Malaysia
publishDate 2015
url http://psasir.upm.edu.my/id/eprint/51955/1/Reduction%20of%20MTT%20to%20purple%20formazan%20by%20vitamin%20E%20isomers%20in%20the%20absence%20of%20cells.pdf
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score 13.4562235

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