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The effect of heat stress on the oxidative status of red hybrid tilapia (Oreochromis sp.) infected with Streptococcus Agalactiae

A commercial red hybrid tilapia was experimented with S. agalactiae infection under influences of heat stress which indicated by plasma malondialdehyde (MDA) and erythrocyte superoxide dismutase (SOD) as biomarkers of stress. To achieve these objectives, 110 red hybrid tilapia in good health were di...

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Main Authors: A. N., Nadirah, M. Y., Sabri, B. O., Emikpe, O., Noraini, P. N., Tanko, O. M., Opeyemi, M. Y., Ina Salwany
Format: Article
Language:English
Published: International Journal of Pharmaceutical Research and Allied Sciences 2016
Online Access:http://psasir.upm.edu.my/id/eprint/52981/1/The%20effect%20of%20heat%20stress%20on%20the%20oxidative%20status%20of%20red%20hybrid%20tilapia%20%28Oreochromis%20sp.%29%20infected%20with%20Streptococcus%20Agalactiae.pdf
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spelling oai:psasir.upm.edu.my:52981 http://psasir.upm.edu.my/id/eprint/52981/ The effect of heat stress on the oxidative status of red hybrid tilapia (Oreochromis sp.) infected with Streptococcus Agalactiae A. N., Nadirah M. Y., Sabri B. O., Emikpe O., Noraini P. N., Tanko O. M., Opeyemi M. Y., Ina Salwany A commercial red hybrid tilapia was experimented with S. agalactiae infection under influences of heat stress which indicated by plasma malondialdehyde (MDA) and erythrocyte superoxide dismutase (SOD) as biomarkers of stress. To achieve these objectives, 110 red hybrid tilapia in good health were divided into five groups of 22 fish each. Group A was challenged with 2.3  109 CFU of S. agalactiae and exposed to heat stress at 33 ± 0.5C on day 1. Group B was challenged on day 1 as in Group A but heat stress was introduced on day 7 post challenge (pc). Group C was exposed to heat stress on day 1 and challenged on day 7 pc while groups D and E served as a positive and negative controls respectively. Blood samples were collected at days 0, 3, 7, 10 and 14 for MDA and SOD analysis. Groups A and B recorded high mortality following exposure to heat stress and bacteria inoculation, with group A reaching 100% mortality at day 7 post inoculation. Overall, Groups A, B, C and Group D showed pattern of increase in MDA level as early as day 3 and decrease pattern for SOD activity. Group E did not show any significant difference in MDA level throughout the study period. Clinical signs such as erratic swimming, exopthalmia, skin haemorrhage and cloudy eye were predominantly observed in group A 24 h post inoculation. Based on the findings of this study, it was concluded that heat stress plays crucial role in the pathogenesis of S. agalactiae, via alteration of the oxidant defence system. International Journal of Pharmaceutical Research and Allied Sciences 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/52981/1/The%20effect%20of%20heat%20stress%20on%20the%20oxidative%20status%20of%20red%20hybrid%20tilapia%20%28Oreochromis%20sp.%29%20infected%20with%20Streptococcus%20Agalactiae.pdf A. N., Nadirah and M. Y., Sabri and B. O., Emikpe and O., Noraini and P. N., Tanko and O. M., Opeyemi and M. Y., Ina Salwany (2016) The effect of heat stress on the oxidative status of red hybrid tilapia (Oreochromis sp.) infected with Streptococcus Agalactiae. International Journal of Pharmaceutical Research and Allied Sciences, 5 (4). pp. 1-14. ISSN 2277-3657 http://www.ijpras.com
institution UPM IR
collection UPM IR
language English
description A commercial red hybrid tilapia was experimented with S. agalactiae infection under influences of heat stress which indicated by plasma malondialdehyde (MDA) and erythrocyte superoxide dismutase (SOD) as biomarkers of stress. To achieve these objectives, 110 red hybrid tilapia in good health were divided into five groups of 22 fish each. Group A was challenged with 2.3  109 CFU of S. agalactiae and exposed to heat stress at 33 ± 0.5C on day 1. Group B was challenged on day 1 as in Group A but heat stress was introduced on day 7 post challenge (pc). Group C was exposed to heat stress on day 1 and challenged on day 7 pc while groups D and E served as a positive and negative controls respectively. Blood samples were collected at days 0, 3, 7, 10 and 14 for MDA and SOD analysis. Groups A and B recorded high mortality following exposure to heat stress and bacteria inoculation, with group A reaching 100% mortality at day 7 post inoculation. Overall, Groups A, B, C and Group D showed pattern of increase in MDA level as early as day 3 and decrease pattern for SOD activity. Group E did not show any significant difference in MDA level throughout the study period. Clinical signs such as erratic swimming, exopthalmia, skin haemorrhage and cloudy eye were predominantly observed in group A 24 h post inoculation. Based on the findings of this study, it was concluded that heat stress plays crucial role in the pathogenesis of S. agalactiae, via alteration of the oxidant defence system.
format Article
author A. N., Nadirah
M. Y., Sabri
B. O., Emikpe
O., Noraini
P. N., Tanko
O. M., Opeyemi
M. Y., Ina Salwany
spellingShingle A. N., Nadirah
M. Y., Sabri
B. O., Emikpe
O., Noraini
P. N., Tanko
O. M., Opeyemi
M. Y., Ina Salwany
The effect of heat stress on the oxidative status of red hybrid tilapia (Oreochromis sp.) infected with Streptococcus Agalactiae
author_facet A. N., Nadirah
M. Y., Sabri
B. O., Emikpe
O., Noraini
P. N., Tanko
O. M., Opeyemi
M. Y., Ina Salwany
author_sort A. N., Nadirah
title The effect of heat stress on the oxidative status of red hybrid tilapia (Oreochromis sp.) infected with Streptococcus Agalactiae
title_short The effect of heat stress on the oxidative status of red hybrid tilapia (Oreochromis sp.) infected with Streptococcus Agalactiae
title_full The effect of heat stress on the oxidative status of red hybrid tilapia (Oreochromis sp.) infected with Streptococcus Agalactiae
title_fullStr The effect of heat stress on the oxidative status of red hybrid tilapia (Oreochromis sp.) infected with Streptococcus Agalactiae
title_full_unstemmed The effect of heat stress on the oxidative status of red hybrid tilapia (Oreochromis sp.) infected with Streptococcus Agalactiae
title_sort effect of heat stress on the oxidative status of red hybrid tilapia (oreochromis sp.) infected with streptococcus agalactiae
publisher International Journal of Pharmaceutical Research and Allied Sciences
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/52981/1/The%20effect%20of%20heat%20stress%20on%20the%20oxidative%20status%20of%20red%20hybrid%20tilapia%20%28Oreochromis%20sp.%29%20infected%20with%20Streptococcus%20Agalactiae.pdf
_version_ 1819297302563520512
score 13.4562235

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