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Modulation of hydrogen peroxide-induced oxidative stress in human neuronal cells by Thymoquinone-rich fraction and Thymoquinone via transcriptomic regulation of antioxidant and apoptotic signaling genes

Nigella sativa Linn. (N. sativa) and its bioactive constituent Thymoquinone (TQ) have demonstrated numerous pharmacological attributes. In the present study, the neuroprotective properties of Thymoquinone-rich fraction (TQRF) and TQ against hydrogen peroxide- (H2O2-) induced neurotoxicity in differe...

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Hoofdauteurs: Ismail, Norsharina, Ismail, Maznah, Azmi, Nur Hanisah, Abu Bakar, Muhammad Firdaus, Basri, Hamidon, Abdullah, Maizaton Atmadini
Formaat: Artikel
Taal:English
Gepubliceerd in: Hindawi Publishing Corporation 2016
Online toegang:http://psasir.upm.edu.my/id/eprint/53316/1/Modulation%20of%20hydrogen%20peroxide-induced%20oxidative%20stress.pdf
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spelling oai:psasir.upm.edu.my:53316 http://psasir.upm.edu.my/id/eprint/53316/ Modulation of hydrogen peroxide-induced oxidative stress in human neuronal cells by Thymoquinone-rich fraction and Thymoquinone via transcriptomic regulation of antioxidant and apoptotic signaling genes Ismail, Norsharina Ismail, Maznah Azmi, Nur Hanisah Abu Bakar, Muhammad Firdaus Basri, Hamidon Abdullah, Maizaton Atmadini Nigella sativa Linn. (N. sativa) and its bioactive constituent Thymoquinone (TQ) have demonstrated numerous pharmacological attributes. In the present study, the neuroprotective properties of Thymoquinone-rich fraction (TQRF) and TQ against hydrogen peroxide- (H2O2-) induced neurotoxicity in differentiated human SH-SY5Y cells were investigated. TQRF was extracted using supercritical fluid extraction while TQ was acquired commercially, and their effects on H2O2 were evaluated using cell viability assay, reactive oxygen species (ROS) assay, morphological observation, and multiplex gene expression. Both TQRF and TQ protected the cells against H2O2 by preserving the mitochondrial metabolic enzymes, reducing intracellular ROS levels, preserving morphological architecture, and modulating the expression of genes related to antioxidants (SOD1, SOD2, and catalase) and signaling genes (p53, AKT1, ERK1/2, p38 MAPK, JNK, and NF-κβ). In conclusion, the enhanced efficacy of TQRF over TQ was likely due to the synergism of multiple constituents in TQRF. The efficacy of TQRF was better than that of TQ alone when equal concentrations of TQ in TQRF were compared. In addition, TQRF also showed comparable effects to TQ when the same concentrations were tested. These findings provide further support for the use of TQRF as an alternative to combat oxidative stress insults in neurodegenerative diseases. Hindawi Publishing Corporation 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/53316/1/Modulation%20of%20hydrogen%20peroxide-induced%20oxidative%20stress.pdf Ismail, Norsharina and Ismail, Maznah and Azmi, Nur Hanisah and Abu Bakar, Muhammad Firdaus and Basri, Hamidon and Abdullah, Maizaton Atmadini (2016) Modulation of hydrogen peroxide-induced oxidative stress in human neuronal cells by Thymoquinone-rich fraction and Thymoquinone via transcriptomic regulation of antioxidant and apoptotic signaling genes. Oxidative Medicine and Cellular Longevity, 2016. art. no. 2528935. pp. 1-15. ISSN 1942-0900; ESSN: 1942-0994 https://www.hindawi.com/journals/omcl/2016/2528935/ 10.1155/2016/2528935
institution UPM IR
collection UPM IR
language English
description Nigella sativa Linn. (N. sativa) and its bioactive constituent Thymoquinone (TQ) have demonstrated numerous pharmacological attributes. In the present study, the neuroprotective properties of Thymoquinone-rich fraction (TQRF) and TQ against hydrogen peroxide- (H2O2-) induced neurotoxicity in differentiated human SH-SY5Y cells were investigated. TQRF was extracted using supercritical fluid extraction while TQ was acquired commercially, and their effects on H2O2 were evaluated using cell viability assay, reactive oxygen species (ROS) assay, morphological observation, and multiplex gene expression. Both TQRF and TQ protected the cells against H2O2 by preserving the mitochondrial metabolic enzymes, reducing intracellular ROS levels, preserving morphological architecture, and modulating the expression of genes related to antioxidants (SOD1, SOD2, and catalase) and signaling genes (p53, AKT1, ERK1/2, p38 MAPK, JNK, and NF-κβ). In conclusion, the enhanced efficacy of TQRF over TQ was likely due to the synergism of multiple constituents in TQRF. The efficacy of TQRF was better than that of TQ alone when equal concentrations of TQ in TQRF were compared. In addition, TQRF also showed comparable effects to TQ when the same concentrations were tested. These findings provide further support for the use of TQRF as an alternative to combat oxidative stress insults in neurodegenerative diseases.
format Article
author Ismail, Norsharina
Ismail, Maznah
Azmi, Nur Hanisah
Abu Bakar, Muhammad Firdaus
Basri, Hamidon
Abdullah, Maizaton Atmadini
spellingShingle Ismail, Norsharina
Ismail, Maznah
Azmi, Nur Hanisah
Abu Bakar, Muhammad Firdaus
Basri, Hamidon
Abdullah, Maizaton Atmadini
Modulation of hydrogen peroxide-induced oxidative stress in human neuronal cells by Thymoquinone-rich fraction and Thymoquinone via transcriptomic regulation of antioxidant and apoptotic signaling genes
author_facet Ismail, Norsharina
Ismail, Maznah
Azmi, Nur Hanisah
Abu Bakar, Muhammad Firdaus
Basri, Hamidon
Abdullah, Maizaton Atmadini
author_sort Ismail, Norsharina
title Modulation of hydrogen peroxide-induced oxidative stress in human neuronal cells by Thymoquinone-rich fraction and Thymoquinone via transcriptomic regulation of antioxidant and apoptotic signaling genes
title_short Modulation of hydrogen peroxide-induced oxidative stress in human neuronal cells by Thymoquinone-rich fraction and Thymoquinone via transcriptomic regulation of antioxidant and apoptotic signaling genes
title_full Modulation of hydrogen peroxide-induced oxidative stress in human neuronal cells by Thymoquinone-rich fraction and Thymoquinone via transcriptomic regulation of antioxidant and apoptotic signaling genes
title_fullStr Modulation of hydrogen peroxide-induced oxidative stress in human neuronal cells by Thymoquinone-rich fraction and Thymoquinone via transcriptomic regulation of antioxidant and apoptotic signaling genes
title_full_unstemmed Modulation of hydrogen peroxide-induced oxidative stress in human neuronal cells by Thymoquinone-rich fraction and Thymoquinone via transcriptomic regulation of antioxidant and apoptotic signaling genes
title_sort modulation of hydrogen peroxide-induced oxidative stress in human neuronal cells by thymoquinone-rich fraction and thymoquinone via transcriptomic regulation of antioxidant and apoptotic signaling genes
publisher Hindawi Publishing Corporation
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/53316/1/Modulation%20of%20hydrogen%20peroxide-induced%20oxidative%20stress.pdf
_version_ 1819297328063840256
score 13.4562235

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