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Molecular characterization of 16S rRNA and internal transcribed spacer (ITS) regions of Aeromonas spp. isolated from cultured freshwater fishes in Malaysia

The Aeromonas spp., which are found mostly in freshwater fishes are known to cause uncontrolled disease outbreak in aquaculture system. In the present study, twenty-two of Aeromonas spp. strains isolated from diseased freshwater fishes were identified using the 16S ribosomal RNA (rRNA) and internal...

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Main Authors: K.P., Diyana Nadhirah, Md Yasin, Ina Salwany
פורמט: Article
שפה:English
יצא לאור: Excellent Publishers 2016
גישה מקוונת:http://psasir.upm.edu.my/id/eprint/53319/1/Molecular%20characterization%20of%2016S%20rRNA%20and%20internal%20transcribed%20spacer%20%28ITS%29%20regions%20of%20Aeromonas%20spp.%20isolated%20from%20cultured%20freshwater%20fishes%20in%20Malaysia.pdf
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id oai:psasir.upm.edu.my:53319
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spelling oai:psasir.upm.edu.my:53319 http://psasir.upm.edu.my/id/eprint/53319/ Molecular characterization of 16S rRNA and internal transcribed spacer (ITS) regions of Aeromonas spp. isolated from cultured freshwater fishes in Malaysia K.P., Diyana Nadhirah Md Yasin, Ina Salwany The Aeromonas spp., which are found mostly in freshwater fishes are known to cause uncontrolled disease outbreak in aquaculture system. In the present study, twenty-two of Aeromonas spp. strains isolated from diseased freshwater fishes were identified using the 16S ribosomal RNA (rRNA) and internal transcribed spacer (ITS) regions gene amplification where they produced a 1500 bp and 1000-1200 bp amplicon each. Phylogenetic analysis with BLASTn and 16S rRNA neighbor-joining bootstrapping methods revealed that all strains were identified up to genus level with 15 strains were classified as Aeromonas hydrophila (A. hydrophila) and the rest were Aeromonas veronii (A. veronii). Further ITS gene analysis disclosed strains identification up to species level; A. hydrophila (14 strains) and A. veronii (8 strains). These rapid DNA-based detection methods are simple, easy to perform and faster in identifying Aeromonas spp., thus are absolutely efficient for regular monitoring of Aeromonas spp. in a potential outbreak situation. Excellent Publishers 2016-09 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/53319/1/Molecular%20characterization%20of%2016S%20rRNA%20and%20internal%20transcribed%20spacer%20%28ITS%29%20regions%20of%20Aeromonas%20spp.%20isolated%20from%20cultured%20freshwater%20fishes%20in%20Malaysia.pdf K.P., Diyana Nadhirah and Md Yasin, Ina Salwany (2016) Molecular characterization of 16S rRNA and internal transcribed spacer (ITS) regions of Aeromonas spp. isolated from cultured freshwater fishes in Malaysia. International Journal of Current Microbiology and Applied Sciences, 5 (9). pp. 431-440. ISSN 2319-7692; ESSN: 2319-7706 https://www.ijcmas.com/abstractview.php?ID=887&vol=5-9-2016&SNo=46 10.20546/ijcmas.2016.509.046
institution UPM IR
collection UPM IR
language English
description The Aeromonas spp., which are found mostly in freshwater fishes are known to cause uncontrolled disease outbreak in aquaculture system. In the present study, twenty-two of Aeromonas spp. strains isolated from diseased freshwater fishes were identified using the 16S ribosomal RNA (rRNA) and internal transcribed spacer (ITS) regions gene amplification where they produced a 1500 bp and 1000-1200 bp amplicon each. Phylogenetic analysis with BLASTn and 16S rRNA neighbor-joining bootstrapping methods revealed that all strains were identified up to genus level with 15 strains were classified as Aeromonas hydrophila (A. hydrophila) and the rest were Aeromonas veronii (A. veronii). Further ITS gene analysis disclosed strains identification up to species level; A. hydrophila (14 strains) and A. veronii (8 strains). These rapid DNA-based detection methods are simple, easy to perform and faster in identifying Aeromonas spp., thus are absolutely efficient for regular monitoring of Aeromonas spp. in a potential outbreak situation.
format Article
author K.P., Diyana Nadhirah
Md Yasin, Ina Salwany
spellingShingle K.P., Diyana Nadhirah
Md Yasin, Ina Salwany
Molecular characterization of 16S rRNA and internal transcribed spacer (ITS) regions of Aeromonas spp. isolated from cultured freshwater fishes in Malaysia
author_facet K.P., Diyana Nadhirah
Md Yasin, Ina Salwany
author_sort K.P., Diyana Nadhirah
title Molecular characterization of 16S rRNA and internal transcribed spacer (ITS) regions of Aeromonas spp. isolated from cultured freshwater fishes in Malaysia
title_short Molecular characterization of 16S rRNA and internal transcribed spacer (ITS) regions of Aeromonas spp. isolated from cultured freshwater fishes in Malaysia
title_full Molecular characterization of 16S rRNA and internal transcribed spacer (ITS) regions of Aeromonas spp. isolated from cultured freshwater fishes in Malaysia
title_fullStr Molecular characterization of 16S rRNA and internal transcribed spacer (ITS) regions of Aeromonas spp. isolated from cultured freshwater fishes in Malaysia
title_full_unstemmed Molecular characterization of 16S rRNA and internal transcribed spacer (ITS) regions of Aeromonas spp. isolated from cultured freshwater fishes in Malaysia
title_sort molecular characterization of 16s rrna and internal transcribed spacer (its) regions of aeromonas spp. isolated from cultured freshwater fishes in malaysia
publisher Excellent Publishers
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/53319/1/Molecular%20characterization%20of%2016S%20rRNA%20and%20internal%20transcribed%20spacer%20%28ITS%29%20regions%20of%20Aeromonas%20spp.%20isolated%20from%20cultured%20freshwater%20fishes%20in%20Malaysia.pdf
_version_ 1819297328258875392
score 13.4562235

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