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Effects of tocotrienols on sperm parameters, testes weight and ultrastructure in Sprague Dawley rats

Vitamin E is divided equally into 2 families, tocopherols and tocotrienols. Being an antioxidant, vitamin E has been reported to improve disorders related to oxidative damage in many organ systems. Sources of vitamin E can be found in many foods including palm oil which is rich in tocotrienols. Stud...

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Main Authors: Lee, Y. J., Khaza'ai, Huzwah, Sukardi, Sabrina
Format: Article
Sprog:English
Udgivet: International Organization of Scientific Research 2016
Fag:
Effects of tocotrienols; Sperm; Parameters; Testes weight; Ultrastructure; Sprague Dawley rats
Online adgang:http://psasir.upm.edu.my/id/eprint/54117/1/Effects%20of%20tocotrienols%20on%20sperm%20parameters%2C%20testes%20weight%20and%20ultrastructure%20in%20Sprague%20Dawley%20rats.pdf
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spelling oai:psasir.upm.edu.my:54117 http://psasir.upm.edu.my/id/eprint/54117/ Effects of tocotrienols on sperm parameters, testes weight and ultrastructure in Sprague Dawley rats Lee, Y. J. Khaza'ai, Huzwah Sukardi, Sabrina Vitamin E is divided equally into 2 families, tocopherols and tocotrienols. Being an antioxidant, vitamin E has been reported to improve disorders related to oxidative damage in many organ systems. Sources of vitamin E can be found in many foods including palm oil which is rich in tocotrienols. Studies on the effects of vitamin E on male fertility have shown encouraging results. This study was conducted to observe the effects of feeding tocotrienols from palm oil to male Sprague-Dawley rats on sperm parameters, testes weight and sperm ultrastructure. Blood nitric oxide (NO) levels were also measured. Thirty-five rats were separated equally into 5 groups: initial group (sacrificed before experiment for base line values), control group (fed commercial pellets only), vehiclegroup (palm oil), low dose and high dose of tocotrienol treatment groups. The four groups were sacrificed at the end of the six weeks experiment and sperm parameters (motility, viability and count) were measured while sperm ultrastructure was observed via transmission electron microscopy (TEM). Cardiac blood was taken for NO analysis. Testes were also weighed. At high dose, it is found that sperm parameters increased significantly (p<0.05) but there was no significant change in NO readings. Testes weight does not show any changes while TEM showed lowered mitochondrial cristae distortion in both treatment groups. High dose of tocotrienol increase sperm parameters suggesting that the mechanism for better male fertility is related to better cristae membrane integrityin sperm. International Organization of Scientific Research 2016 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/54117/1/Effects%20of%20tocotrienols%20on%20sperm%20parameters%2C%20testes%20weight%20and%20ultrastructure%20in%20Sprague%20Dawley%20rats.pdf Lee, Y. J. and Khaza'ai, Huzwah and Sukardi, Sabrina (2016) Effects of tocotrienols on sperm parameters, testes weight and ultrastructure in Sprague Dawley rats. IOSR Journal of Pharmacy and Biological Sciences, 11 (6). pp. 73-79. ISSN 2319-7676; ESSN: 2278-3008 http://www.iosrjournals.org/iosr-jpbs/pages/11(6)Version-4.html Effects of tocotrienols; Sperm; Parameters; Testes weight; Ultrastructure; Sprague Dawley rats 10.9790/3008 - 110604 7 3 7 9
institution UPM IR
collection UPM IR
language English
topic Effects of tocotrienols; Sperm; Parameters; Testes weight; Ultrastructure; Sprague Dawley rats
spellingShingle Effects of tocotrienols; Sperm; Parameters; Testes weight; Ultrastructure; Sprague Dawley rats
Lee, Y. J.
Khaza'ai, Huzwah
Sukardi, Sabrina
Effects of tocotrienols on sperm parameters, testes weight and ultrastructure in Sprague Dawley rats
description Vitamin E is divided equally into 2 families, tocopherols and tocotrienols. Being an antioxidant, vitamin E has been reported to improve disorders related to oxidative damage in many organ systems. Sources of vitamin E can be found in many foods including palm oil which is rich in tocotrienols. Studies on the effects of vitamin E on male fertility have shown encouraging results. This study was conducted to observe the effects of feeding tocotrienols from palm oil to male Sprague-Dawley rats on sperm parameters, testes weight and sperm ultrastructure. Blood nitric oxide (NO) levels were also measured. Thirty-five rats were separated equally into 5 groups: initial group (sacrificed before experiment for base line values), control group (fed commercial pellets only), vehiclegroup (palm oil), low dose and high dose of tocotrienol treatment groups. The four groups were sacrificed at the end of the six weeks experiment and sperm parameters (motility, viability and count) were measured while sperm ultrastructure was observed via transmission electron microscopy (TEM). Cardiac blood was taken for NO analysis. Testes were also weighed. At high dose, it is found that sperm parameters increased significantly (p<0.05) but there was no significant change in NO readings. Testes weight does not show any changes while TEM showed lowered mitochondrial cristae distortion in both treatment groups. High dose of tocotrienol increase sperm parameters suggesting that the mechanism for better male fertility is related to better cristae membrane integrityin sperm.
format Article
author Lee, Y. J.
Khaza'ai, Huzwah
Sukardi, Sabrina
author_facet Lee, Y. J.
Khaza'ai, Huzwah
Sukardi, Sabrina
author_sort Lee, Y. J.
title Effects of tocotrienols on sperm parameters, testes weight and ultrastructure in Sprague Dawley rats
title_short Effects of tocotrienols on sperm parameters, testes weight and ultrastructure in Sprague Dawley rats
title_full Effects of tocotrienols on sperm parameters, testes weight and ultrastructure in Sprague Dawley rats
title_fullStr Effects of tocotrienols on sperm parameters, testes weight and ultrastructure in Sprague Dawley rats
title_full_unstemmed Effects of tocotrienols on sperm parameters, testes weight and ultrastructure in Sprague Dawley rats
title_sort effects of tocotrienols on sperm parameters, testes weight and ultrastructure in sprague dawley rats
publisher International Organization of Scientific Research
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/54117/1/Effects%20of%20tocotrienols%20on%20sperm%20parameters%2C%20testes%20weight%20and%20ultrastructure%20in%20Sprague%20Dawley%20rats.pdf
_version_ 1819297413923340288
score 13.4562235

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