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Evaluation of wound healing properties of bioactive aqueous fraction from Moringa oleifera Lam on experimentally induced diabetic animal model

Diabetic foot ulcer is a serious complication of diabetes, which affects a significant percentage (15%) of diabetics and up to 15%-24% of those affected may require amputation. Therefore, the economic burden of diabetic foot ulcers is enormous and is associated with high cost of treatment and prolon...

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Main Authors: Muhammad, Abubakar Amali, Arulselvan, Palanisamy, Cheah, Pike See, Abas, Faridah, Fakurazi, Sharida
Format: Article
Language:English
Published: Dove Medical Press 2016
Subjects:
Aqueous fraction; Vicenin-2; Wound healing; Inflammatory mediators
Online Access:http://psasir.upm.edu.my/id/eprint/54218/1/Evaluation%20of%20wound%20healing%20properties%20of%20bioactive%20aqueous.pdf
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spelling oai:psasir.upm.edu.my:54218 http://psasir.upm.edu.my/id/eprint/54218/ Evaluation of wound healing properties of bioactive aqueous fraction from Moringa oleifera Lam on experimentally induced diabetic animal model Muhammad, Abubakar Amali Arulselvan, Palanisamy Cheah, Pike See Abas, Faridah Fakurazi, Sharida Diabetic foot ulcer is a serious complication of diabetes, which affects a significant percentage (15%) of diabetics and up to 15%-24% of those affected may require amputation. Therefore, the economic burden of diabetic foot ulcers is enormous and is associated with high cost of treatment and prolongs hospitalization. The present study was conducted to evaluate antibacterial and in vivo wound healing activities of an aqueous fraction of Moringa oleifera on a diabetic condition. Antibacterial activity testing was carried out using agar well and tube dilution techniques. The in vivo study was conducted using six groups of animals that comprise of one normal and diabetic control group each, three treatment groups of 0.5%, 1%, and 2% w/w aqueous fraction, and a positive control group (1% w/w silver sulfadiazine). Rats were induced with diabetes using a combination of streptozotocin 65 and 150 mg/kg nicotinamide daily for 2 days, and excision wounds were created and treated with various doses (0.5%, 1%, and 2% w/w aqueous fraction) daily for 21 days. Biophysical, histological, and biochemical parameters were investigated. The results of the study revealed that aqueous fraction possessed antibacterial activity through inhibition of growth of Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli organisms. The topical application of aqueous fraction revealed enhancement of wound healing under sustained hyperglycemic condition for the duration of the experiment. This enhancement was achieved through decreased wound size, improved wound contraction, and tissue regeneration, as well as downregulation of inflammatory mediators, such as tumor necrosis factor-α, interleukin-1β, interleukin-6, inducible nitric oxide synthase, and cyclooxygenase-2, and upregulation of an angiogenic marker vascular endothelial growth factor in wound tissue treated with various doses of aqueous fraction of M. oleifera. The findings suggest that aqueous fraction of M. oleifera containing Vicenin-2 active compound may accelerate wound healing in hyperglycemic condition. Dove Medical Press 2016 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/54218/1/Evaluation%20of%20wound%20healing%20properties%20of%20bioactive%20aqueous.pdf Muhammad, Abubakar Amali and Arulselvan, Palanisamy and Cheah, Pike See and Abas, Faridah and Fakurazi, Sharida (2016) Evaluation of wound healing properties of bioactive aqueous fraction from Moringa oleifera Lam on experimentally induced diabetic animal model. Drug Design, Development and Therapy, 10. pp. 1715-1730. ISSN 1177-8881 https://www.dovepress.com/evaluation-of-wound-healing-properties-of-bioactive-aqueous-fraction-f-peer-reviewed-article-DDDT Aqueous fraction; Vicenin-2; Wound healing; Inflammatory mediators 10.2147/DDDT.S96968
institution UPM IR
collection UPM IR
language English
topic Aqueous fraction; Vicenin-2; Wound healing; Inflammatory mediators
spellingShingle Aqueous fraction; Vicenin-2; Wound healing; Inflammatory mediators
Muhammad, Abubakar Amali
Arulselvan, Palanisamy
Cheah, Pike See
Abas, Faridah
Fakurazi, Sharida
Evaluation of wound healing properties of bioactive aqueous fraction from Moringa oleifera Lam on experimentally induced diabetic animal model
description Diabetic foot ulcer is a serious complication of diabetes, which affects a significant percentage (15%) of diabetics and up to 15%-24% of those affected may require amputation. Therefore, the economic burden of diabetic foot ulcers is enormous and is associated with high cost of treatment and prolongs hospitalization. The present study was conducted to evaluate antibacterial and in vivo wound healing activities of an aqueous fraction of Moringa oleifera on a diabetic condition. Antibacterial activity testing was carried out using agar well and tube dilution techniques. The in vivo study was conducted using six groups of animals that comprise of one normal and diabetic control group each, three treatment groups of 0.5%, 1%, and 2% w/w aqueous fraction, and a positive control group (1% w/w silver sulfadiazine). Rats were induced with diabetes using a combination of streptozotocin 65 and 150 mg/kg nicotinamide daily for 2 days, and excision wounds were created and treated with various doses (0.5%, 1%, and 2% w/w aqueous fraction) daily for 21 days. Biophysical, histological, and biochemical parameters were investigated. The results of the study revealed that aqueous fraction possessed antibacterial activity through inhibition of growth of Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli organisms. The topical application of aqueous fraction revealed enhancement of wound healing under sustained hyperglycemic condition for the duration of the experiment. This enhancement was achieved through decreased wound size, improved wound contraction, and tissue regeneration, as well as downregulation of inflammatory mediators, such as tumor necrosis factor-α, interleukin-1β, interleukin-6, inducible nitric oxide synthase, and cyclooxygenase-2, and upregulation of an angiogenic marker vascular endothelial growth factor in wound tissue treated with various doses of aqueous fraction of M. oleifera. The findings suggest that aqueous fraction of M. oleifera containing Vicenin-2 active compound may accelerate wound healing in hyperglycemic condition.
format Article
author Muhammad, Abubakar Amali
Arulselvan, Palanisamy
Cheah, Pike See
Abas, Faridah
Fakurazi, Sharida
author_facet Muhammad, Abubakar Amali
Arulselvan, Palanisamy
Cheah, Pike See
Abas, Faridah
Fakurazi, Sharida
author_sort Muhammad, Abubakar Amali
title Evaluation of wound healing properties of bioactive aqueous fraction from Moringa oleifera Lam on experimentally induced diabetic animal model
title_short Evaluation of wound healing properties of bioactive aqueous fraction from Moringa oleifera Lam on experimentally induced diabetic animal model
title_full Evaluation of wound healing properties of bioactive aqueous fraction from Moringa oleifera Lam on experimentally induced diabetic animal model
title_fullStr Evaluation of wound healing properties of bioactive aqueous fraction from Moringa oleifera Lam on experimentally induced diabetic animal model
title_full_unstemmed Evaluation of wound healing properties of bioactive aqueous fraction from Moringa oleifera Lam on experimentally induced diabetic animal model
title_sort evaluation of wound healing properties of bioactive aqueous fraction from moringa oleifera lam on experimentally induced diabetic animal model
publisher Dove Medical Press
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/54218/1/Evaluation%20of%20wound%20healing%20properties%20of%20bioactive%20aqueous.pdf
_version_ 1782723273281241088
score 12.935284

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