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Immunohistochemical evaluation of lesions in the gastrointestinal tract of buffalo (Bubalus bubalis) calves orally exposed to Pasteurella multocida B:2

The gastrointestinal lesions and bacterial distribution of buffalo calves were evaluated histologically using immunoperoxidase, following oral exposure to wild-type Pasteurella multocida B:2 at 109cfu/mL in phosphate buffered saline. The lesions were basically of mild to severe mucohaemorrhagic ab...

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Main Authors: Abubakar, Muhammad Salisu, Saad, Mohd Zamri, Sabri, Jasni, Abu Bakar @ Zakaria, Md Zuki
Format: Article
Language:English
Published: Faculty of Veterinary Medicine, Usmanu Danfodiyo University 2016
Subjects:
Buffalo calves; Gastrointestinal lesions; Immunoperoxidase; Oral infection; Pasteurella multocida B:2
Online Access:http://psasir.upm.edu.my/id/eprint/54497/1/Immunohistochemical%20evaluation%20of%20lesions%20in%20the%20gastrointestinal%20tract%20of%20buffalo%20.pdf
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spelling oai:psasir.upm.edu.my:54497 http://psasir.upm.edu.my/id/eprint/54497/ Immunohistochemical evaluation of lesions in the gastrointestinal tract of buffalo (Bubalus bubalis) calves orally exposed to Pasteurella multocida B:2 Abubakar, Muhammad Salisu Saad, Mohd Zamri Sabri, Jasni Abu Bakar @ Zakaria, Md Zuki The gastrointestinal lesions and bacterial distribution of buffalo calves were evaluated histologically using immunoperoxidase, following oral exposure to wild-type Pasteurella multocida B:2 at 109cfu/mL in phosphate buffered saline. The lesions were basically of mild to severe mucohaemorrhagic abomasitis and enteritis.The lesions were confirmed to be associated with the inoculated P. multocida B:2, using the immunoperoxidase technique. P. multocida B:2 antigen was detected not only in the bacterial clusters in the gastric pits, intestinal epithelia and capillaries, Brünner’s glands and Crypt of Lieberkühn but was also seen interacting with infiltrating neutrophils and macrophages intracellularly and on the surface of erythrocyte in congested vessels and haemorrhages. We observed higher localization and distribution of the immunoperoxidase reaction with increased severity of lesions along the gastrointestinal tract. This suggest intensity increases with increased amount of P. multocida B:2 or antigen in the tissue, which possibly leads to increase tissue damage. Faculty of Veterinary Medicine, Usmanu Danfodiyo University 2016 Article NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/54497/1/Immunohistochemical%20evaluation%20of%20lesions%20in%20the%20gastrointestinal%20tract%20of%20buffalo%20.pdf Abubakar, Muhammad Salisu and Saad, Mohd Zamri and Sabri, Jasni and Abu Bakar @ Zakaria, Md Zuki (2016) Immunohistochemical evaluation of lesions in the gastrointestinal tract of buffalo (Bubalus bubalis) calves orally exposed to Pasteurella multocida B:2. Sokoto Journal of Veterinary Sciences, 14 (1). pp. 21-27. ISSN 1595-093X; ESSN: 2315-6201 https://www.ajol.info/index.php/sokjvs/article/view/135209 Buffalo calves; Gastrointestinal lesions; Immunoperoxidase; Oral infection; Pasteurella multocida B:2 10.4314/sokjvs.v14i1.4
institution UPM IR
collection UPM IR
language English
topic Buffalo calves; Gastrointestinal lesions; Immunoperoxidase; Oral infection; Pasteurella multocida B:2
spellingShingle Buffalo calves; Gastrointestinal lesions; Immunoperoxidase; Oral infection; Pasteurella multocida B:2
Abubakar, Muhammad Salisu
Saad, Mohd Zamri
Sabri, Jasni
Abu Bakar @ Zakaria, Md Zuki
Immunohistochemical evaluation of lesions in the gastrointestinal tract of buffalo (Bubalus bubalis) calves orally exposed to Pasteurella multocida B:2
description The gastrointestinal lesions and bacterial distribution of buffalo calves were evaluated histologically using immunoperoxidase, following oral exposure to wild-type Pasteurella multocida B:2 at 109cfu/mL in phosphate buffered saline. The lesions were basically of mild to severe mucohaemorrhagic abomasitis and enteritis.The lesions were confirmed to be associated with the inoculated P. multocida B:2, using the immunoperoxidase technique. P. multocida B:2 antigen was detected not only in the bacterial clusters in the gastric pits, intestinal epithelia and capillaries, Brünner’s glands and Crypt of Lieberkühn but was also seen interacting with infiltrating neutrophils and macrophages intracellularly and on the surface of erythrocyte in congested vessels and haemorrhages. We observed higher localization and distribution of the immunoperoxidase reaction with increased severity of lesions along the gastrointestinal tract. This suggest intensity increases with increased amount of P. multocida B:2 or antigen in the tissue, which possibly leads to increase tissue damage.
format Article
author Abubakar, Muhammad Salisu
Saad, Mohd Zamri
Sabri, Jasni
Abu Bakar @ Zakaria, Md Zuki
author_facet Abubakar, Muhammad Salisu
Saad, Mohd Zamri
Sabri, Jasni
Abu Bakar @ Zakaria, Md Zuki
author_sort Abubakar, Muhammad Salisu
title Immunohistochemical evaluation of lesions in the gastrointestinal tract of buffalo (Bubalus bubalis) calves orally exposed to Pasteurella multocida B:2
title_short Immunohistochemical evaluation of lesions in the gastrointestinal tract of buffalo (Bubalus bubalis) calves orally exposed to Pasteurella multocida B:2
title_full Immunohistochemical evaluation of lesions in the gastrointestinal tract of buffalo (Bubalus bubalis) calves orally exposed to Pasteurella multocida B:2
title_fullStr Immunohistochemical evaluation of lesions in the gastrointestinal tract of buffalo (Bubalus bubalis) calves orally exposed to Pasteurella multocida B:2
title_full_unstemmed Immunohistochemical evaluation of lesions in the gastrointestinal tract of buffalo (Bubalus bubalis) calves orally exposed to Pasteurella multocida B:2
title_sort immunohistochemical evaluation of lesions in the gastrointestinal tract of buffalo (bubalus bubalis) calves orally exposed to pasteurella multocida b:2
publisher Faculty of Veterinary Medicine, Usmanu Danfodiyo University
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/54497/1/Immunohistochemical%20evaluation%20of%20lesions%20in%20the%20gastrointestinal%20tract%20of%20buffalo%20.pdf
_version_ 1819297454792638464
score 13.4562235

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