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Isolation and metagenomic identification of Avian leukosis virus associated with mortality in broiler chicken

Avian leukosis virus (ALV) belongs to the family Retroviridae and causes considerable economic losses to the poultry industry. Following an outbreak associated with high mortality in a broiler flock in northern part of Malaysia, kidney tissues from affected chickens were submitted for virus isolatio...

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Egile Nagusiak: Bande, Faruku, Arshad, Siti Suri, Omar, Abdul Rahman
Formatua: Artikulua
Hizkuntza:English
Argitaratua: Hindawi Publishing Corporation 2016
Sarrera elektronikoa:http://psasir.upm.edu.my/id/eprint/54637/1/Isolation%20and%20metagenomic%20identification%20of%20Avian%20leukosis%20virus%20associated%20with%20mortality%20in%20broiler%20chicken.pdf
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spelling oai:psasir.upm.edu.my:54637 http://psasir.upm.edu.my/id/eprint/54637/ Isolation and metagenomic identification of Avian leukosis virus associated with mortality in broiler chicken Bande, Faruku Arshad, Siti Suri Omar, Abdul Rahman Avian leukosis virus (ALV) belongs to the family Retroviridae and causes considerable economic losses to the poultry industry. Following an outbreak associated with high mortality in a broiler flock in northern part of Malaysia, kidney tissues from affected chickens were submitted for virus isolation and identification in chicken embryonated egg and MDCK cells. Evidence of virus growth was indicated by haemorrhage and embryo mortality in egg culture. While viral growth in cell culture was evidenced by the development of cytopathic effects. The isolated virus was purified by sucrose gradient and identified using negative staining transmission electron microscopy. Further confirmation was achieved through next-generation sequencing and nucleotide sequence homology search. Analysis of the viral sequences using the NCBI BLAST tool revealed 99-100% sequence homology with exogenous ALV viral envelope protein. Phylogenetic analysis based on partial envelope sequences showed the Malaysian isolate clustered with Taiwanese and Japanese ALV strains, which were closer to ALV subgroup J, ALV subgroup E, and recombinant A/E isolates. Based on these findings, ALV was concluded to be associated with the present outbreak. It was recommended that further studies should be conducted on the molecular epidemiology and pathogenicity of the identified virus isolate. Hindawi Publishing Corporation 2016 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/54637/1/Isolation%20and%20metagenomic%20identification%20of%20Avian%20leukosis%20virus%20associated%20with%20mortality%20in%20broiler%20chicken.pdf Bande, Faruku and Arshad, Siti Suri and Omar, Abdul Rahman (2016) Isolation and metagenomic identification of Avian leukosis virus associated with mortality in broiler chicken. Advances in Virology, 2016. art. no. 9058403. pp. 1-4. ISSN 1687-8639; ESSN: 1687-8647 https://www.hindawi.com/journals/av/2016/9058403/abs/ 10.1155/2016/9058403
institution UPM IR
collection UPM IR
language English
description Avian leukosis virus (ALV) belongs to the family Retroviridae and causes considerable economic losses to the poultry industry. Following an outbreak associated with high mortality in a broiler flock in northern part of Malaysia, kidney tissues from affected chickens were submitted for virus isolation and identification in chicken embryonated egg and MDCK cells. Evidence of virus growth was indicated by haemorrhage and embryo mortality in egg culture. While viral growth in cell culture was evidenced by the development of cytopathic effects. The isolated virus was purified by sucrose gradient and identified using negative staining transmission electron microscopy. Further confirmation was achieved through next-generation sequencing and nucleotide sequence homology search. Analysis of the viral sequences using the NCBI BLAST tool revealed 99-100% sequence homology with exogenous ALV viral envelope protein. Phylogenetic analysis based on partial envelope sequences showed the Malaysian isolate clustered with Taiwanese and Japanese ALV strains, which were closer to ALV subgroup J, ALV subgroup E, and recombinant A/E isolates. Based on these findings, ALV was concluded to be associated with the present outbreak. It was recommended that further studies should be conducted on the molecular epidemiology and pathogenicity of the identified virus isolate.
format Article
author Bande, Faruku
Arshad, Siti Suri
Omar, Abdul Rahman
spellingShingle Bande, Faruku
Arshad, Siti Suri
Omar, Abdul Rahman
Isolation and metagenomic identification of Avian leukosis virus associated with mortality in broiler chicken
author_facet Bande, Faruku
Arshad, Siti Suri
Omar, Abdul Rahman
author_sort Bande, Faruku
title Isolation and metagenomic identification of Avian leukosis virus associated with mortality in broiler chicken
title_short Isolation and metagenomic identification of Avian leukosis virus associated with mortality in broiler chicken
title_full Isolation and metagenomic identification of Avian leukosis virus associated with mortality in broiler chicken
title_fullStr Isolation and metagenomic identification of Avian leukosis virus associated with mortality in broiler chicken
title_full_unstemmed Isolation and metagenomic identification of Avian leukosis virus associated with mortality in broiler chicken
title_sort isolation and metagenomic identification of avian leukosis virus associated with mortality in broiler chicken
publisher Hindawi Publishing Corporation
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/54637/1/Isolation%20and%20metagenomic%20identification%20of%20Avian%20leukosis%20virus%20associated%20with%20mortality%20in%20broiler%20chicken.pdf
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score 13.4562235

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