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Bioactive constituents of Zanthoxylum rhetsa bark and its cytotoxic potential against B16-F10 melanoma cancer and normal human dermal fibroblast (HDF) cell lines

Zanthoxylum rhetsa is an aromatic tree, known vernacularly as "Indian Prickly Ash". It has been predominantly used by Indian tribes for the treatment of many infirmities like diabetes, inflammation, rheumatism, toothache and diarrhea. In this study, we identified major volatile constituent...

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Main Authors: Santhanam, Ramesh Kumar, Ahmad, Syahida, Abas, Faridah, Ismail, Intan Safinar, Rukayadi, Yaya, Akhtar, Muhammad Tayyab, Shaari, Khozirah
Format: Article
Language:English
Published: MDPI 2016
Online Access:http://psasir.upm.edu.my/id/eprint/55201/1/Bioactive%20constituents%20of%20Zanthoxylum%20rhetsa%20bark%20and%20its%20cytotoxic%20potential%20against%20B16-F10%20melanoma%20cancer%20and%20normal%20human%20dermal%20fibroblast%20%28HDF%29%20cell%20lines.pdf
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spelling oai:psasir.upm.edu.my:55201 http://psasir.upm.edu.my/id/eprint/55201/ Bioactive constituents of Zanthoxylum rhetsa bark and its cytotoxic potential against B16-F10 melanoma cancer and normal human dermal fibroblast (HDF) cell lines Santhanam, Ramesh Kumar Ahmad, Syahida Abas, Faridah Ismail, Intan Safinar Rukayadi, Yaya Akhtar, Muhammad Tayyab Shaari, Khozirah Zanthoxylum rhetsa is an aromatic tree, known vernacularly as "Indian Prickly Ash". It has been predominantly used by Indian tribes for the treatment of many infirmities like diabetes, inflammation, rheumatism, toothache and diarrhea. In this study, we identified major volatile constituents present in different solvent fractions of Z. rhetsa bark using GC-MS analysis and isolated two tetrahydrofuran lignans (yangambin and kobusin), a berberine alkaloid (columbamine) and a triterpenoid (lupeol) from the bioactive chloroform fraction. The solvent fractions and purified compounds were tested for their cytotoxic potential against human dermal fibroblasts (HDF) and mouse melanoma (B16-F10) cells, using the MTT assay. All the solvent fractions and purified compounds were found to be non-cytotoxic to HDF cells. However, the chloroform fraction and kobusin exhibited cytotoxic effect against B16-F10 melanoma cells. The presence of bioactive lignans and alkaloids were suggested to be responsible for the cytotoxic property of Z. rhetsa bark against B16-F10 cells. MDPI 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/55201/1/Bioactive%20constituents%20of%20Zanthoxylum%20rhetsa%20bark%20and%20its%20cytotoxic%20potential%20against%20B16-F10%20melanoma%20cancer%20and%20normal%20human%20dermal%20fibroblast%20%28HDF%29%20cell%20lines.pdf Santhanam, Ramesh Kumar and Ahmad, Syahida and Abas, Faridah and Ismail, Intan Safinar and Rukayadi, Yaya and Akhtar, Muhammad Tayyab and Shaari, Khozirah (2016) Bioactive constituents of Zanthoxylum rhetsa bark and its cytotoxic potential against B16-F10 melanoma cancer and normal human dermal fibroblast (HDF) cell lines. Molecules, 21 (652). pp. 1-13. ISSN 1431-5157; ESSN: 1420-3049 http://www.mdpi.com/journal/molecules 10.3390/molecules21060652
institution UPM IR
collection UPM IR
language English
description Zanthoxylum rhetsa is an aromatic tree, known vernacularly as "Indian Prickly Ash". It has been predominantly used by Indian tribes for the treatment of many infirmities like diabetes, inflammation, rheumatism, toothache and diarrhea. In this study, we identified major volatile constituents present in different solvent fractions of Z. rhetsa bark using GC-MS analysis and isolated two tetrahydrofuran lignans (yangambin and kobusin), a berberine alkaloid (columbamine) and a triterpenoid (lupeol) from the bioactive chloroform fraction. The solvent fractions and purified compounds were tested for their cytotoxic potential against human dermal fibroblasts (HDF) and mouse melanoma (B16-F10) cells, using the MTT assay. All the solvent fractions and purified compounds were found to be non-cytotoxic to HDF cells. However, the chloroform fraction and kobusin exhibited cytotoxic effect against B16-F10 melanoma cells. The presence of bioactive lignans and alkaloids were suggested to be responsible for the cytotoxic property of Z. rhetsa bark against B16-F10 cells.
format Article
author Santhanam, Ramesh Kumar
Ahmad, Syahida
Abas, Faridah
Ismail, Intan Safinar
Rukayadi, Yaya
Akhtar, Muhammad Tayyab
Shaari, Khozirah
spellingShingle Santhanam, Ramesh Kumar
Ahmad, Syahida
Abas, Faridah
Ismail, Intan Safinar
Rukayadi, Yaya
Akhtar, Muhammad Tayyab
Shaari, Khozirah
Bioactive constituents of Zanthoxylum rhetsa bark and its cytotoxic potential against B16-F10 melanoma cancer and normal human dermal fibroblast (HDF) cell lines
author_facet Santhanam, Ramesh Kumar
Ahmad, Syahida
Abas, Faridah
Ismail, Intan Safinar
Rukayadi, Yaya
Akhtar, Muhammad Tayyab
Shaari, Khozirah
author_sort Santhanam, Ramesh Kumar
title Bioactive constituents of Zanthoxylum rhetsa bark and its cytotoxic potential against B16-F10 melanoma cancer and normal human dermal fibroblast (HDF) cell lines
title_short Bioactive constituents of Zanthoxylum rhetsa bark and its cytotoxic potential against B16-F10 melanoma cancer and normal human dermal fibroblast (HDF) cell lines
title_full Bioactive constituents of Zanthoxylum rhetsa bark and its cytotoxic potential against B16-F10 melanoma cancer and normal human dermal fibroblast (HDF) cell lines
title_fullStr Bioactive constituents of Zanthoxylum rhetsa bark and its cytotoxic potential against B16-F10 melanoma cancer and normal human dermal fibroblast (HDF) cell lines
title_full_unstemmed Bioactive constituents of Zanthoxylum rhetsa bark and its cytotoxic potential against B16-F10 melanoma cancer and normal human dermal fibroblast (HDF) cell lines
title_sort bioactive constituents of zanthoxylum rhetsa bark and its cytotoxic potential against b16-f10 melanoma cancer and normal human dermal fibroblast (hdf) cell lines
publisher MDPI
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/55201/1/Bioactive%20constituents%20of%20Zanthoxylum%20rhetsa%20bark%20and%20its%20cytotoxic%20potential%20against%20B16-F10%20melanoma%20cancer%20and%20normal%20human%20dermal%20fibroblast%20%28HDF%29%20cell%20lines.pdf
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score 13.4562235

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