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Combination of VP3 and CD147-knockdown enhance apoptosis and tumor growth delay index in colorectal tumor allograft

Background: Cancer therapies that kill cancer cells without affecting normal cells is the ultimate mode of treating cancers. The VP3, an avian virus-derived protein, can specifically initiate cell death through several signal transduction pathways leading to apoptosis. In cancer, chemoresistance and...

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Main Authors: Ismail, Ruzila, Allaudin, Zeenathul Nazariah, Abdullah, Rasedee, Mohd Lila, Mohd Azmi, Nik Abd Rahman, Nik Mohd Afizan, Abdul Rahman, Sheikh Omar
Format: Article
Language:English
Published: BioMed Central 2016
Online Access:http://psasir.upm.edu.my/id/eprint/55310/1/Combination%20of%20VP3%20and%20CD147-knockdown.pdf
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spelling oai:psasir.upm.edu.my:55310 http://psasir.upm.edu.my/id/eprint/55310/ Combination of VP3 and CD147-knockdown enhance apoptosis and tumor growth delay index in colorectal tumor allograft Ismail, Ruzila Allaudin, Zeenathul Nazariah Abdullah, Rasedee Mohd Lila, Mohd Azmi Nik Abd Rahman, Nik Mohd Afizan Abdul Rahman, Sheikh Omar Background: Cancer therapies that kill cancer cells without affecting normal cells is the ultimate mode of treating cancers. The VP3, an avian virus-derived protein, can specifically initiate cell death through several signal transduction pathways leading to apoptosis. In cancer, chemoresistance and cell survivability implicate the cell surface protein, CD147. Methods: In this study, transfection of VP3 and silencing of CD147 genes was achieved through the treatment of tumors with pVIVO1-GFP/VP3 (VP3), psiRNA-CD147/2 (shCD147/2), and their combination of CT26 colon cancer cell-induced in mice. The effectiveness of tumor-treatment was ascertained by electrophoresis, TUNEL assay, and flow cytometry analysis. While histopathological and biochemical analysis were used as toxic side effect identification. Results: The tumor growth delay index (TGDI) after treatment with VP3, shCD147/2, and their combination treatments increased by 1.3-, 1.2-, 2.0- and 2.3-fold respectively, over untreated control. The VP3-shCD147/2 combination treatment was more efficacious then either VP3 or shCD147/2 alone in the retardation of mouse CT26 colorectal cell tumor allograft. Conclusion: The antitumor effect of the combination treatment is the result of synergistic effects of VP3 and shCD147/2 on the tumor cells resulting in apoptosis. Thus, the study shows that combination of VP3 and shCD147/2 treatment can be developed into a potential approach for anticolorectal cancer treatment regimen. BioMed Central 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/55310/1/Combination%20of%20VP3%20and%20CD147-knockdown.pdf Ismail, Ruzila and Allaudin, Zeenathul Nazariah and Abdullah, Rasedee and Mohd Lila, Mohd Azmi and Nik Abd Rahman, Nik Mohd Afizan and Abdul Rahman, Sheikh Omar (2016) Combination of VP3 and CD147-knockdown enhance apoptosis and tumor growth delay index in colorectal tumor allograft. BMC Cancer, 16. art. no. 461. pp. 1-13. ISSN 1471-2407 https://bmccancer.biomedcentral.com/track/pdf/10.1186/s12885-016-2530-8?site=bmccancer.biomedcentral.com 10.1186/s12885-016-2530-8
institution UPM IR
collection UPM IR
language English
description Background: Cancer therapies that kill cancer cells without affecting normal cells is the ultimate mode of treating cancers. The VP3, an avian virus-derived protein, can specifically initiate cell death through several signal transduction pathways leading to apoptosis. In cancer, chemoresistance and cell survivability implicate the cell surface protein, CD147. Methods: In this study, transfection of VP3 and silencing of CD147 genes was achieved through the treatment of tumors with pVIVO1-GFP/VP3 (VP3), psiRNA-CD147/2 (shCD147/2), and their combination of CT26 colon cancer cell-induced in mice. The effectiveness of tumor-treatment was ascertained by electrophoresis, TUNEL assay, and flow cytometry analysis. While histopathological and biochemical analysis were used as toxic side effect identification. Results: The tumor growth delay index (TGDI) after treatment with VP3, shCD147/2, and their combination treatments increased by 1.3-, 1.2-, 2.0- and 2.3-fold respectively, over untreated control. The VP3-shCD147/2 combination treatment was more efficacious then either VP3 or shCD147/2 alone in the retardation of mouse CT26 colorectal cell tumor allograft. Conclusion: The antitumor effect of the combination treatment is the result of synergistic effects of VP3 and shCD147/2 on the tumor cells resulting in apoptosis. Thus, the study shows that combination of VP3 and shCD147/2 treatment can be developed into a potential approach for anticolorectal cancer treatment regimen.
format Article
author Ismail, Ruzila
Allaudin, Zeenathul Nazariah
Abdullah, Rasedee
Mohd Lila, Mohd Azmi
Nik Abd Rahman, Nik Mohd Afizan
Abdul Rahman, Sheikh Omar
spellingShingle Ismail, Ruzila
Allaudin, Zeenathul Nazariah
Abdullah, Rasedee
Mohd Lila, Mohd Azmi
Nik Abd Rahman, Nik Mohd Afizan
Abdul Rahman, Sheikh Omar
Combination of VP3 and CD147-knockdown enhance apoptosis and tumor growth delay index in colorectal tumor allograft
author_facet Ismail, Ruzila
Allaudin, Zeenathul Nazariah
Abdullah, Rasedee
Mohd Lila, Mohd Azmi
Nik Abd Rahman, Nik Mohd Afizan
Abdul Rahman, Sheikh Omar
author_sort Ismail, Ruzila
title Combination of VP3 and CD147-knockdown enhance apoptosis and tumor growth delay index in colorectal tumor allograft
title_short Combination of VP3 and CD147-knockdown enhance apoptosis and tumor growth delay index in colorectal tumor allograft
title_full Combination of VP3 and CD147-knockdown enhance apoptosis and tumor growth delay index in colorectal tumor allograft
title_fullStr Combination of VP3 and CD147-knockdown enhance apoptosis and tumor growth delay index in colorectal tumor allograft
title_full_unstemmed Combination of VP3 and CD147-knockdown enhance apoptosis and tumor growth delay index in colorectal tumor allograft
title_sort combination of vp3 and cd147-knockdown enhance apoptosis and tumor growth delay index in colorectal tumor allograft
publisher BioMed Central
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/55310/1/Combination%20of%20VP3%20and%20CD147-knockdown.pdf
_version_ 1782723421586587648
score 12.935284

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