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Detection of tuberculosis (TB) using gold standard method, direct sputum smears microscopy, PCR, qPCR and electrochemical DNA sensor: a mini review

Despite the continued effort globally made to control the growing case of Tuberculosis (TB), it continues to be regarded as the second deadliest disease after the HIV. There are various methods developed to diagnose TB, most of which having the criteria of sensitive, selective, cheap and portable to...

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Главные авторы: Che-Engku-Chik, Che Engku Noramalina, Yusof, Nor Azah, Abdullah, Jaafar, Othman, Siti Sarah, Mat Said, Mohd Hazani, Wasoh, Helmi
Формат: Статья
Язык:English
Опубликовано: Hibiscus Publisher 2016
Online-ссылка:http://psasir.upm.edu.my/id/eprint/55462/1/Detection%20of%20tuberculosis%20%28TB%29%20using%20gold%20standard%20method%2C%20direct%20sputum%20smears%20.pdf
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spelling oai:psasir.upm.edu.my:55462 http://psasir.upm.edu.my/id/eprint/55462/ Detection of tuberculosis (TB) using gold standard method, direct sputum smears microscopy, PCR, qPCR and electrochemical DNA sensor: a mini review Che-Engku-Chik, Che Engku Noramalina Yusof, Nor Azah Abdullah, Jaafar Othman, Siti Sarah Mat Said, Mohd Hazani Wasoh, Helmi Despite the continued effort globally made to control the growing case of Tuberculosis (TB), it continues to be regarded as the second deadliest disease after the HIV. There are various methods developed to diagnose TB, most of which having the criteria of sensitive, selective, cheap and portable to be used in robust applications. Even with the advancement in medication, the important keys including early stage diagnosis is yet to be considered. In diagnosing TB, the only technique remained as the gold standard method is the culturing method, which is the Acid Fast Bacilli (AFB) staining. On the other hand, molecular technique utilising Polymerase Chain Reaction (PCR) assay is preferred as a non-culturing method. Additionally, as molecular techniques become advanced, real-time PCR or quantitative PCR (qPCR) using multiple probes in one shot has raised interest among researchers, because it can skip the process of gel electrophoresis. Recently, researchers have been working on electrochemical DNA sensors which are sensitive, selective, rapid, cheap and can meet with point of care (POC) testing requirements to diagnose TB. Hibiscus Publisher 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/55462/1/Detection%20of%20tuberculosis%20%28TB%29%20using%20gold%20standard%20method%2C%20direct%20sputum%20smears%20.pdf Che-Engku-Chik, Che Engku Noramalina and Yusof, Nor Azah and Abdullah, Jaafar and Othman, Siti Sarah and Mat Said, Mohd Hazani and Wasoh, Helmi (2016) Detection of tuberculosis (TB) using gold standard method, direct sputum smears microscopy, PCR, qPCR and electrochemical DNA sensor: a mini review. Journal of Biochemistry, Microbiology and Biotechnology, 4 (2). pp. 16-21. ISSN 2289-5779 http://journal.hibiscuspublisher.com/index.php/JOBIMB/index
institution UPM IR
collection UPM IR
language English
description Despite the continued effort globally made to control the growing case of Tuberculosis (TB), it continues to be regarded as the second deadliest disease after the HIV. There are various methods developed to diagnose TB, most of which having the criteria of sensitive, selective, cheap and portable to be used in robust applications. Even with the advancement in medication, the important keys including early stage diagnosis is yet to be considered. In diagnosing TB, the only technique remained as the gold standard method is the culturing method, which is the Acid Fast Bacilli (AFB) staining. On the other hand, molecular technique utilising Polymerase Chain Reaction (PCR) assay is preferred as a non-culturing method. Additionally, as molecular techniques become advanced, real-time PCR or quantitative PCR (qPCR) using multiple probes in one shot has raised interest among researchers, because it can skip the process of gel electrophoresis. Recently, researchers have been working on electrochemical DNA sensors which are sensitive, selective, rapid, cheap and can meet with point of care (POC) testing requirements to diagnose TB.
format Article
author Che-Engku-Chik, Che Engku Noramalina
Yusof, Nor Azah
Abdullah, Jaafar
Othman, Siti Sarah
Mat Said, Mohd Hazani
Wasoh, Helmi
spellingShingle Che-Engku-Chik, Che Engku Noramalina
Yusof, Nor Azah
Abdullah, Jaafar
Othman, Siti Sarah
Mat Said, Mohd Hazani
Wasoh, Helmi
Detection of tuberculosis (TB) using gold standard method, direct sputum smears microscopy, PCR, qPCR and electrochemical DNA sensor: a mini review
author_facet Che-Engku-Chik, Che Engku Noramalina
Yusof, Nor Azah
Abdullah, Jaafar
Othman, Siti Sarah
Mat Said, Mohd Hazani
Wasoh, Helmi
author_sort Che-Engku-Chik, Che Engku Noramalina
title Detection of tuberculosis (TB) using gold standard method, direct sputum smears microscopy, PCR, qPCR and electrochemical DNA sensor: a mini review
title_short Detection of tuberculosis (TB) using gold standard method, direct sputum smears microscopy, PCR, qPCR and electrochemical DNA sensor: a mini review
title_full Detection of tuberculosis (TB) using gold standard method, direct sputum smears microscopy, PCR, qPCR and electrochemical DNA sensor: a mini review
title_fullStr Detection of tuberculosis (TB) using gold standard method, direct sputum smears microscopy, PCR, qPCR and electrochemical DNA sensor: a mini review
title_full_unstemmed Detection of tuberculosis (TB) using gold standard method, direct sputum smears microscopy, PCR, qPCR and electrochemical DNA sensor: a mini review
title_sort detection of tuberculosis (tb) using gold standard method, direct sputum smears microscopy, pcr, qpcr and electrochemical dna sensor: a mini review
publisher Hibiscus Publisher
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/55462/1/Detection%20of%20tuberculosis%20%28TB%29%20using%20gold%20standard%20method%2C%20direct%20sputum%20smears%20.pdf
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score 13.4562235

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