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Determination of predominate Leptospira serovars circulating among rat populations in Kuala Lumpur

Leptospirosis is re-emerging in Malaysia and many other tropical countries. Its infection of human and animal’s worldwide. The study was carried out to identify predominate Leptospira serovars within rat populations in Kuala Lumpur (KL) by microscopic agglutination test (MAT). As well to investiga...

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Główni autorzy: Al Kattan, Ghada, Bahaman, Abdul Rani, Bejo, Siti Khairani, Mutalib, Abdul Rahim, Zakaria, Zunita
Format: Artykuł
Język:English
Wydane: Veterinary Association Malaysia 2017
Dostęp online:http://psasir.upm.edu.my/id/eprint/58227/1/JVM-2017-Issue-2_Kattan-and-Rani.pdf
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spelling oai:psasir.upm.edu.my:58227 http://psasir.upm.edu.my/id/eprint/58227/ Determination of predominate Leptospira serovars circulating among rat populations in Kuala Lumpur Al Kattan, Ghada Bahaman, Abdul Rani Bejo, Siti Khairani Mutalib, Abdul Rahim Zakaria, Zunita Leptospirosis is re-emerging in Malaysia and many other tropical countries. Its infection of human and animal’s worldwide. The study was carried out to identify predominate Leptospira serovars within rat populations in Kuala Lumpur (KL) by microscopic agglutination test (MAT). As well to investigate the frequency of infection by polymerase chain reaction assays (PCR). The isolated cultures that obtained from culturing kidney tissue of 112 trapped rats from four sites in KL, were identified by MAT using panel of 16 standard hyper immune anti-sera. Besides, identification of Leptospira strains by PCR amplification with G1/G2 and ompL1 Borgpetersenii genes. Results from typing by MAT revealed that 8/57 (7.1%) of cultured isolates reacted against; hyper-immune antisera of serogroup Javanica and 13/57 (22.8%) against serogroup Bataviae. Whereas, the rest of isolates recorded across reactivity 1/20 against serogroups; Icterohaemorrhagiae 2/57, Canicola 1/57, Australis 2/57. From PCR-G1/G2; 50/112 (50.9%) cultures were positive included (40/57 cultures positive to isolation and 10/55 cultures negative to isolation). While, from PCR-ompL1 Borgpetersenii gene; 21/112 (18.7%) cultures positive included (17/57 cultures positive to isolation and 4/55 cultures were negative to isolation). It is obvious from this study that serogroups Javanica and Bataviae were the predominant among rat populations in KL. Also it revealed the high frequency of pathogenic strains among rat populations and their potential risk of humans and animals contracting infection. Veterinary Association Malaysia 2017 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/58227/1/JVM-2017-Issue-2_Kattan-and-Rani.pdf Al Kattan, Ghada and Bahaman, Abdul Rani and Bejo, Siti Khairani and Mutalib, Abdul Rahim and Zakaria, Zunita (2017) Determination of predominate Leptospira serovars circulating among rat populations in Kuala Lumpur. Jurnal Veterinar Malaysia, 29 (2). pp. 1-4. ISSN 9128-2506 http://jvm.vam.org.my/wp-content/uploads/2017/12/JVM-2017-Issue-2_Kattan-and-Rani.pdf
institution UPM IR
collection UPM IR
language English
description Leptospirosis is re-emerging in Malaysia and many other tropical countries. Its infection of human and animal’s worldwide. The study was carried out to identify predominate Leptospira serovars within rat populations in Kuala Lumpur (KL) by microscopic agglutination test (MAT). As well to investigate the frequency of infection by polymerase chain reaction assays (PCR). The isolated cultures that obtained from culturing kidney tissue of 112 trapped rats from four sites in KL, were identified by MAT using panel of 16 standard hyper immune anti-sera. Besides, identification of Leptospira strains by PCR amplification with G1/G2 and ompL1 Borgpetersenii genes. Results from typing by MAT revealed that 8/57 (7.1%) of cultured isolates reacted against; hyper-immune antisera of serogroup Javanica and 13/57 (22.8%) against serogroup Bataviae. Whereas, the rest of isolates recorded across reactivity 1/20 against serogroups; Icterohaemorrhagiae 2/57, Canicola 1/57, Australis 2/57. From PCR-G1/G2; 50/112 (50.9%) cultures were positive included (40/57 cultures positive to isolation and 10/55 cultures negative to isolation). While, from PCR-ompL1 Borgpetersenii gene; 21/112 (18.7%) cultures positive included (17/57 cultures positive to isolation and 4/55 cultures were negative to isolation). It is obvious from this study that serogroups Javanica and Bataviae were the predominant among rat populations in KL. Also it revealed the high frequency of pathogenic strains among rat populations and their potential risk of humans and animals contracting infection.
format Article
author Al Kattan, Ghada
Bahaman, Abdul Rani
Bejo, Siti Khairani
Mutalib, Abdul Rahim
Zakaria, Zunita
spellingShingle Al Kattan, Ghada
Bahaman, Abdul Rani
Bejo, Siti Khairani
Mutalib, Abdul Rahim
Zakaria, Zunita
Determination of predominate Leptospira serovars circulating among rat populations in Kuala Lumpur
author_facet Al Kattan, Ghada
Bahaman, Abdul Rani
Bejo, Siti Khairani
Mutalib, Abdul Rahim
Zakaria, Zunita
author_sort Al Kattan, Ghada
title Determination of predominate Leptospira serovars circulating among rat populations in Kuala Lumpur
title_short Determination of predominate Leptospira serovars circulating among rat populations in Kuala Lumpur
title_full Determination of predominate Leptospira serovars circulating among rat populations in Kuala Lumpur
title_fullStr Determination of predominate Leptospira serovars circulating among rat populations in Kuala Lumpur
title_full_unstemmed Determination of predominate Leptospira serovars circulating among rat populations in Kuala Lumpur
title_sort determination of predominate leptospira serovars circulating among rat populations in kuala lumpur
publisher Veterinary Association Malaysia
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/58227/1/JVM-2017-Issue-2_Kattan-and-Rani.pdf
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score 13.4562235

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