Antibiotic resistance profiles of Staphylococcus pseudintermedius isolated from dogs and cats

Aims: Antibiotic resistance in Staphylococcus pseudintermedius is increasing gradually towards those antibiotics that are frequently used leading to limited therapeutic options due to multidrug resistance. The objectives of the study were to investigate the antibiotic resistance profiles of S. pseud...

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Hoofdauteurs: Mohamed, Mohamed Abdelrahman, Abdul Aziz, Saleha, Dhaliwal, Gurmeet Kaur, Bejo, Siti Khairani, Goni, Mohammed Dauda, Bitrus, Asinamai Athliamai, Muhammad, Ibrahim Jalo
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Taal:English
Gepubliceerd in: Malaysian Society for Microbiology 2017
Online toegang:http://psasir.upm.edu.my/id/eprint/60604/1/Antibiotic%20resistance%20profiles%20of%20Staphylococcus%20pseudintermedius%20isolated%20from%20dogs%20and%20cats.pdf
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spelling oai:psasir.upm.edu.my:60604 http://psasir.upm.edu.my/id/eprint/60604/ Antibiotic resistance profiles of Staphylococcus pseudintermedius isolated from dogs and cats Mohamed, Mohamed Abdelrahman Abdul Aziz, Saleha Dhaliwal, Gurmeet Kaur Bejo, Siti Khairani Goni, Mohammed Dauda Bitrus, Asinamai Athliamai Muhammad, Ibrahim Jalo Aims: Antibiotic resistance in Staphylococcus pseudintermedius is increasing gradually towards those antibiotics that are frequently used leading to limited therapeutic options due to multidrug resistance. The objectives of the study were to investigate the antibiotic resistance profiles of S. pseudintermedius isolates from pet and stray dogs and cats in Selangor, Malaysia and to detect the resistance genes (mecA and BlaZ) within the isolates. Methodology and results: A total of 200 stray and pet dogs and cats were sampled. The samples were cultured onto Mannitol Salt agar and all the presumptive colonies were subcultured, then identified using biochemical tests and confirmed by PCR assay targeting the nuc gene. The isolates were subjected to antibiotic susceptibility test against 12 antibiotics. Twenty three isolates (11.5%) were positive to S. pseudintermedius (stray cats, 11/50; stray dogs, 9/50; pet dogs, 3/50 and pet cats, 0/50). One hundred percent (100%) of the S. pseudintermedius isolates were found to be resistant to penicillin, erythromycin and tetracycline while they showed 100% susceptible to oxacillin, amoxicillinclavulanic acid, gentamicin, chloramphenicol, vancomycin, ciprofloxacin, enrofloxacin, cephalexin and rifampicin. The blaZ gene which codes for β-lactamases production was found in all of the isolates that were resistant to penicillin but not to methicillin. Conclusion, significance and impact of study: A high number of S. pseudintermedius from dogs and cats developed antibiotic resistance which is a public health concern. Malaysian Society for Microbiology 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/60604/1/Antibiotic%20resistance%20profiles%20of%20Staphylococcus%20pseudintermedius%20isolated%20from%20dogs%20and%20cats.pdf Mohamed, Mohamed Abdelrahman and Abdul Aziz, Saleha and Dhaliwal, Gurmeet Kaur and Bejo, Siti Khairani and Goni, Mohammed Dauda and Bitrus, Asinamai Athliamai and Muhammad, Ibrahim Jalo (2017) Antibiotic resistance profiles of Staphylococcus pseudintermedius isolated from dogs and cats. Malaysian Journal of Microbiology, 13 (3). pp. 180-186. ISSN 1823-8262; ESSN: 2231-7538 http://mjm.usm.my/index.php?r=cms/entry/view&id=51
institution UPM IR
collection UPM IR
language English
description Aims: Antibiotic resistance in Staphylococcus pseudintermedius is increasing gradually towards those antibiotics that are frequently used leading to limited therapeutic options due to multidrug resistance. The objectives of the study were to investigate the antibiotic resistance profiles of S. pseudintermedius isolates from pet and stray dogs and cats in Selangor, Malaysia and to detect the resistance genes (mecA and BlaZ) within the isolates. Methodology and results: A total of 200 stray and pet dogs and cats were sampled. The samples were cultured onto Mannitol Salt agar and all the presumptive colonies were subcultured, then identified using biochemical tests and confirmed by PCR assay targeting the nuc gene. The isolates were subjected to antibiotic susceptibility test against 12 antibiotics. Twenty three isolates (11.5%) were positive to S. pseudintermedius (stray cats, 11/50; stray dogs, 9/50; pet dogs, 3/50 and pet cats, 0/50). One hundred percent (100%) of the S. pseudintermedius isolates were found to be resistant to penicillin, erythromycin and tetracycline while they showed 100% susceptible to oxacillin, amoxicillinclavulanic acid, gentamicin, chloramphenicol, vancomycin, ciprofloxacin, enrofloxacin, cephalexin and rifampicin. The blaZ gene which codes for β-lactamases production was found in all of the isolates that were resistant to penicillin but not to methicillin. Conclusion, significance and impact of study: A high number of S. pseudintermedius from dogs and cats developed antibiotic resistance which is a public health concern.
format Article
author Mohamed, Mohamed Abdelrahman
Abdul Aziz, Saleha
Dhaliwal, Gurmeet Kaur
Bejo, Siti Khairani
Goni, Mohammed Dauda
Bitrus, Asinamai Athliamai
Muhammad, Ibrahim Jalo
spellingShingle Mohamed, Mohamed Abdelrahman
Abdul Aziz, Saleha
Dhaliwal, Gurmeet Kaur
Bejo, Siti Khairani
Goni, Mohammed Dauda
Bitrus, Asinamai Athliamai
Muhammad, Ibrahim Jalo
Antibiotic resistance profiles of Staphylococcus pseudintermedius isolated from dogs and cats
author_facet Mohamed, Mohamed Abdelrahman
Abdul Aziz, Saleha
Dhaliwal, Gurmeet Kaur
Bejo, Siti Khairani
Goni, Mohammed Dauda
Bitrus, Asinamai Athliamai
Muhammad, Ibrahim Jalo
author_sort Mohamed, Mohamed Abdelrahman
title Antibiotic resistance profiles of Staphylococcus pseudintermedius isolated from dogs and cats
title_short Antibiotic resistance profiles of Staphylococcus pseudintermedius isolated from dogs and cats
title_full Antibiotic resistance profiles of Staphylococcus pseudintermedius isolated from dogs and cats
title_fullStr Antibiotic resistance profiles of Staphylococcus pseudintermedius isolated from dogs and cats
title_full_unstemmed Antibiotic resistance profiles of Staphylococcus pseudintermedius isolated from dogs and cats
title_sort antibiotic resistance profiles of staphylococcus pseudintermedius isolated from dogs and cats
publisher Malaysian Society for Microbiology
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/60604/1/Antibiotic%20resistance%20profiles%20of%20Staphylococcus%20pseudintermedius%20isolated%20from%20dogs%20and%20cats.pdf
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score 13.4562235