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A transmission electron microscopy study of anticoagulant-induced platelet vesiculation

Platelet microparticles (PMPs) are small membrane fragments released from activated platelets in response to various stimuli. PMPs serve as biomarkers for several diseases and conditions and are useful tools for prognostic, diagnostic, and therapeutic purposes. The objective of our study was to comp...

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主要な著者: Almhanawai, Bahaa Hadi Jaber, Khalid, Bahariah, Tengku Ibrahim, Tengku Azmi, Mohd Tohit, Eusni Rahayu
フォーマット: 論文
言語:English
出版事項: PBJ-Associaco Porto Biomedical 2017
オンライン･アクセス:http://psasir.upm.edu.my/id/eprint/60752/1/A%20transmission%20electron%20microscopy%20study%20of%20anticoagulant-induced%20platelet%20vesiculation.pdf
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spelling oai:psasir.upm.edu.my:60752 http://psasir.upm.edu.my/id/eprint/60752/ A transmission electron microscopy study of anticoagulant-induced platelet vesiculation Almhanawai, Bahaa Hadi Jaber Khalid, Bahariah Tengku Ibrahim, Tengku Azmi Mohd Tohit, Eusni Rahayu Platelet microparticles (PMPs) are small membrane fragments released from activated platelets in response to various stimuli. PMPs serve as biomarkers for several diseases and conditions and are useful tools for prognostic, diagnostic, and therapeutic purposes. The objective of our study was to compare the direct effects of ethylenediaminetetraacetic acid (EDTA) and sodium citrate anticoagulants on platelet structure and PMP vesiculation using transmission electron microscopy to visualize the morphologic changes in platelets. Micrographs revealed that platelets in the EDTA-anticoagulated tube manifested with significant morphologic changes and induced PMP vesiculation. On the other hand, the sodium citrate-anticoagulated tube showed a normal platelet structure and minor modifications in some cases, with poor indication of PMP vesiculation. In conclusion, EDTA induced platelet activation and PMP vesiculation and represents a major source of artifacts during the pre-analysis steps of PMP vesiculation. PBJ-Associaco Porto Biomedical 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/60752/1/A%20transmission%20electron%20microscopy%20study%20of%20anticoagulant-induced%20platelet%20vesiculation.pdf Almhanawai, Bahaa Hadi Jaber and Khalid, Bahariah and Tengku Ibrahim, Tengku Azmi and Mohd Tohit, Eusni Rahayu (2017) A transmission electron microscopy study of anticoagulant-induced platelet vesiculation. Porto Biomedical Journal, 2 (1). 23 - 27. ISSN 2444-8664 https://reader.elsevier.com/reader/sd/pii/S2444866416300964?token=66A46483B82856C546F3C428841960FFF0B96099E7746896F6944458704B7A04F5010626F9684494DCAA4530E9602E61 10.1016/j.pbj.2016.11.002
institution UPM IR
collection UPM IR
language English
description Platelet microparticles (PMPs) are small membrane fragments released from activated platelets in response to various stimuli. PMPs serve as biomarkers for several diseases and conditions and are useful tools for prognostic, diagnostic, and therapeutic purposes. The objective of our study was to compare the direct effects of ethylenediaminetetraacetic acid (EDTA) and sodium citrate anticoagulants on platelet structure and PMP vesiculation using transmission electron microscopy to visualize the morphologic changes in platelets. Micrographs revealed that platelets in the EDTA-anticoagulated tube manifested with significant morphologic changes and induced PMP vesiculation. On the other hand, the sodium citrate-anticoagulated tube showed a normal platelet structure and minor modifications in some cases, with poor indication of PMP vesiculation. In conclusion, EDTA induced platelet activation and PMP vesiculation and represents a major source of artifacts during the pre-analysis steps of PMP vesiculation.
format Article
author Almhanawai, Bahaa Hadi Jaber
Khalid, Bahariah
Tengku Ibrahim, Tengku Azmi
Mohd Tohit, Eusni Rahayu
spellingShingle Almhanawai, Bahaa Hadi Jaber
Khalid, Bahariah
Tengku Ibrahim, Tengku Azmi
Mohd Tohit, Eusni Rahayu
A transmission electron microscopy study of anticoagulant-induced platelet vesiculation
author_facet Almhanawai, Bahaa Hadi Jaber
Khalid, Bahariah
Tengku Ibrahim, Tengku Azmi
Mohd Tohit, Eusni Rahayu
author_sort Almhanawai, Bahaa Hadi Jaber
title A transmission electron microscopy study of anticoagulant-induced platelet vesiculation
title_short A transmission electron microscopy study of anticoagulant-induced platelet vesiculation
title_full A transmission electron microscopy study of anticoagulant-induced platelet vesiculation
title_fullStr A transmission electron microscopy study of anticoagulant-induced platelet vesiculation
title_full_unstemmed A transmission electron microscopy study of anticoagulant-induced platelet vesiculation
title_sort transmission electron microscopy study of anticoagulant-induced platelet vesiculation
publisher PBJ-Associaco Porto Biomedical
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/60752/1/A%20transmission%20electron%20microscopy%20study%20of%20anticoagulant-induced%20platelet%20vesiculation.pdf
_version_ 1819298059707744256
score 13.4562235

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