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Antibiotic profiling of methicillin resistant Staphylococcus aureus (MRSA) isolates in stray canines and felines

Background: Methicillin resistant Staphylococcus aureus (MRSA) is an important Gram positive pathogen that has raised concerns due to its increasing prevalence despite pharmaceutical and technological advances. It does not only cause infections in humans but it can also be zoonotic in nature. The ai...

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Main Authors: Kanagarajah, Rajwin Raja, Lee, David Charles Weerasingam, Zhi, Daniel Fung Lee, Yusoff, Khatijah, Paramasivam, Sharmini Julita, Wai, Yee Low, Jeevaratnam, Kamalan, Swee, Hua Erin Lim
Format: Article
Language:English
Published: Cogent Biology 2017
Online Access:http://psasir.upm.edu.my/id/eprint/60880/1/Antibiotic%20profiling%20of%20methicillin%20resistant%20Staphylococcus%20aureus%20%28MRSA%29%20isolates%20in%20stray%20canines%20and%20felines.pdf
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spelling oai:psasir.upm.edu.my:60880 http://psasir.upm.edu.my/id/eprint/60880/ Antibiotic profiling of methicillin resistant Staphylococcus aureus (MRSA) isolates in stray canines and felines Kanagarajah, Rajwin Raja Lee, David Charles Weerasingam Zhi, Daniel Fung Lee Yusoff, Khatijah Paramasivam, Sharmini Julita Wai, Yee Low Jeevaratnam, Kamalan Swee, Hua Erin Lim Background: Methicillin resistant Staphylococcus aureus (MRSA) is an important Gram positive pathogen that has raised concerns due to its increasing prevalence despite pharmaceutical and technological advances. It does not only cause infections in humans but it can also be zoonotic in nature. The aim of this study is to investigate the prevalence of MRSA from presumably healthy shelter animals, in particular, canines and felines. Methods: Fifty-two faecal samples from canines and felines were collected from an animal shelter for the isolation of MRSA. This was carried out using the ChromMRSA (Oxoid, United Kingdom) media, followed by antibiotic susceptibility testing using the Kirby–Bauer disk diffusion test in accordance to the Clinical and Laboratory Standards Institute (CLSI) for ceftazidime, enrofloxacin, methicillin, oxacillin and vancomycin. Principal component analysis (PCA) was then employed to identify the variables in antibiotic sensitivity and emphasise any patterns between isolates and the antibiotic profile from both animal samples types. This was then summarised using descriptive statistics. Results: 283 and 169 S.aureus isolates were obtained respectively from the canine and feline samples on selective media. Of this, 33/283 (11.66%) and 13/169 (7.70%) were MRSA when grown on ChromMRSA. Canine MRSA isolates exhibited resistance in decreasing order of methicillin (100%), ceftazidime (81.82%), enrofloxacin (78.79%), oxacillin (60.61%) and vancomycin (0%). On the same note feline MRSA isolates indicated resistance to methicillin (100%), ceftazidime (100%), enrofloxacin (92.31%), oxacillin (84.62%) and vancomycin (0%). 51.51% of the canine and 84.62% of feline MRSA isolates indicated resistance to four out of five antibiotics tested. PCA of antibiotic resistance profiles revealed that canine and feline formed distinct groups. However, one of the feline MRSA isolates resembled more of the canine group; although likelihood of cross-transmission between the animals may be low due to separate enclosures for the canines and felines: cross-transmission may have occurred when animals are brought into the main building for vaccination, neutering and consulting procedures. Conclusion: The majority of MRSA isolates obtained were not only resistant to methicillin alone but to other antibiotics too. Vancomycin proved to be the only effective antibiotic. This will pose a greater risk of resistance developing in empirical antibiotics in the future if proper antibiotic stewardship practices are not in place. Cogent Biology 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/60880/1/Antibiotic%20profiling%20of%20methicillin%20resistant%20Staphylococcus%20aureus%20%28MRSA%29%20isolates%20in%20stray%20canines%20and%20felines.pdf Kanagarajah, Rajwin Raja and Lee, David Charles Weerasingam and Zhi, Daniel Fung Lee and Yusoff, Khatijah and Paramasivam, Sharmini Julita and Wai, Yee Low and Jeevaratnam, Kamalan and Swee, Hua Erin Lim (2017) Antibiotic profiling of methicillin resistant Staphylococcus aureus (MRSA) isolates in stray canines and felines. Microbiology, Parasitology & Virology, 3. art. no. 1412280. pp. 1-10. ISSN 2331-2025 https://www.cogentoa.com/article/10.1080/23312025.2017.1412280.pdf 10.1080/23312025.2017.1412280
institution UPM IR
collection UPM IR
language English
description Background: Methicillin resistant Staphylococcus aureus (MRSA) is an important Gram positive pathogen that has raised concerns due to its increasing prevalence despite pharmaceutical and technological advances. It does not only cause infections in humans but it can also be zoonotic in nature. The aim of this study is to investigate the prevalence of MRSA from presumably healthy shelter animals, in particular, canines and felines. Methods: Fifty-two faecal samples from canines and felines were collected from an animal shelter for the isolation of MRSA. This was carried out using the ChromMRSA (Oxoid, United Kingdom) media, followed by antibiotic susceptibility testing using the Kirby–Bauer disk diffusion test in accordance to the Clinical and Laboratory Standards Institute (CLSI) for ceftazidime, enrofloxacin, methicillin, oxacillin and vancomycin. Principal component analysis (PCA) was then employed to identify the variables in antibiotic sensitivity and emphasise any patterns between isolates and the antibiotic profile from both animal samples types. This was then summarised using descriptive statistics. Results: 283 and 169 S.aureus isolates were obtained respectively from the canine and feline samples on selective media. Of this, 33/283 (11.66%) and 13/169 (7.70%) were MRSA when grown on ChromMRSA. Canine MRSA isolates exhibited resistance in decreasing order of methicillin (100%), ceftazidime (81.82%), enrofloxacin (78.79%), oxacillin (60.61%) and vancomycin (0%). On the same note feline MRSA isolates indicated resistance to methicillin (100%), ceftazidime (100%), enrofloxacin (92.31%), oxacillin (84.62%) and vancomycin (0%). 51.51% of the canine and 84.62% of feline MRSA isolates indicated resistance to four out of five antibiotics tested. PCA of antibiotic resistance profiles revealed that canine and feline formed distinct groups. However, one of the feline MRSA isolates resembled more of the canine group; although likelihood of cross-transmission between the animals may be low due to separate enclosures for the canines and felines: cross-transmission may have occurred when animals are brought into the main building for vaccination, neutering and consulting procedures. Conclusion: The majority of MRSA isolates obtained were not only resistant to methicillin alone but to other antibiotics too. Vancomycin proved to be the only effective antibiotic. This will pose a greater risk of resistance developing in empirical antibiotics in the future if proper antibiotic stewardship practices are not in place.
format Article
author Kanagarajah, Rajwin Raja
Lee, David Charles Weerasingam
Zhi, Daniel Fung Lee
Yusoff, Khatijah
Paramasivam, Sharmini Julita
Wai, Yee Low
Jeevaratnam, Kamalan
Swee, Hua Erin Lim
spellingShingle Kanagarajah, Rajwin Raja
Lee, David Charles Weerasingam
Zhi, Daniel Fung Lee
Yusoff, Khatijah
Paramasivam, Sharmini Julita
Wai, Yee Low
Jeevaratnam, Kamalan
Swee, Hua Erin Lim
Antibiotic profiling of methicillin resistant Staphylococcus aureus (MRSA) isolates in stray canines and felines
author_facet Kanagarajah, Rajwin Raja
Lee, David Charles Weerasingam
Zhi, Daniel Fung Lee
Yusoff, Khatijah
Paramasivam, Sharmini Julita
Wai, Yee Low
Jeevaratnam, Kamalan
Swee, Hua Erin Lim
author_sort Kanagarajah, Rajwin Raja
title Antibiotic profiling of methicillin resistant Staphylococcus aureus (MRSA) isolates in stray canines and felines
title_short Antibiotic profiling of methicillin resistant Staphylococcus aureus (MRSA) isolates in stray canines and felines
title_full Antibiotic profiling of methicillin resistant Staphylococcus aureus (MRSA) isolates in stray canines and felines
title_fullStr Antibiotic profiling of methicillin resistant Staphylococcus aureus (MRSA) isolates in stray canines and felines
title_full_unstemmed Antibiotic profiling of methicillin resistant Staphylococcus aureus (MRSA) isolates in stray canines and felines
title_sort antibiotic profiling of methicillin resistant staphylococcus aureus (mrsa) isolates in stray canines and felines
publisher Cogent Biology
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/60880/1/Antibiotic%20profiling%20of%20methicillin%20resistant%20Staphylococcus%20aureus%20%28MRSA%29%20isolates%20in%20stray%20canines%20and%20felines.pdf
_version_ 1819298067877199872
score 13.4562235

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