Staff View: Assessment of therapeutic effects of Nigella sativa against chronic lead acetate-induced reproductive dysfunction in male Sprague-Dawley rats

Assessment of therapeutic effects of Nigella sativa against chronic lead acetate-induced reproductive dysfunction in male Sprague-Dawley rats

Lead acetate (LA) is a toxic compound that has detrimental effects on the male reproductive system, such as decreased testicular size and function, low androgen hormone concentration, and altered testicular histology. While Nigella sativa (NS) has been shown to possess a handful of therapeutic benef...

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Main Authors:	Assi, Mohammed Abdulrazzaq, Mohd Noor, Mohd Hezmee, Abba, Yusuf, Rajion, Mohamed Ali, Haron, Abd. Wahid, Mohd Yusoff, Md Sabri
Format:	Article
Language:	English
Published:	Springer 2017
Online Access:	http://psasir.upm.edu.my/id/eprint/60950/1/Assessment%20of%20therapeutic%20effects%20of%20Nigella%20sativa%20against%20chronic%20lead%20acetate-induced%20reproductive%20dysfunction%20in%20male%20Sprague-Dawley%20rats.pdf
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spelling	oai:psasir.upm.edu.my:60950 http://psasir.upm.edu.my/id/eprint/60950/ Assessment of therapeutic effects of Nigella sativa against chronic lead acetate-induced reproductive dysfunction in male Sprague-Dawley rats Assi, Mohammed Abdulrazzaq Mohd Noor, Mohd Hezmee Abba, Yusuf Rajion, Mohamed Ali Haron, Abd. Wahid Mohd Yusoff, Md Sabri Lead acetate (LA) is a toxic compound that has detrimental effects on the male reproductive system, such as decreased testicular size and function, low androgen hormone concentration, and altered testicular histology. While Nigella sativa (NS) has been shown to possess a handful of therapeutic benefits, few studies have shown its effect on damage caused by lead acetate toxicity in the male reproductive system. In this study, 75 Sprague-Dawley rats were divided into 3 groups of 25 rats, which were further subdivided into 5 subgroups each. Group 1 (negative control) was given distilled water, group 2 (positive control (PC)) administered 10 mg/kg of lead acetate (LA) orally/daily, while groups 3 (T1), 4 (T2), and 5 (T3) were given LA 10 mg/kg and graded concentrations (100, 150, and 200 mg/kg) each of NS. One group each, comprising of 25 rats, was euthanized at days 30, 60, and 90 for collection of blood plasma, epididymis, and organ tissues fixed in 10 % buffered formalin at each time interval. The right caudal epididymis was homogenized and used for the determination of spermiogram. Plasma was used for the determination of testosterone (TS), follicle stimulating hormone (FSH), and luteinizing hormone (LH) and estradiol (EST) using radioimmunoassay kits. There was reduced number of spermatogenic cells and epididymal sperm reserves in the PC group in comparison to the treatment groups. The level of TS was lower (p < 0.05) in the PC group at 90 days, while FSH was lower (p < 0.05) in T3 at 30 days and LH was higher (p < 0.05) in T1 at 90 days. The concentration of EST was lower (p < 0.05) in the PC, T1, and T2 at all time points, while the T3 group had the higher EST concentration that was similar to the control group. There was a decreased level of superoxide dismutase (SOD) and total glutathione (GSH) in the PC group and an increased GSH level in the T3 group. Sperm concentration, viability, and motility were adversely affected by LA, while concurrent treatment with NS significantly (p < 0.05) improved these parameters. This study showed the detrimental effects of LA on spermatogenesis, TS levels, and antioxidant defenses; however, these adverse effects were alleviated by oral NS administration. Springer 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/60950/1/Assessment%20of%20therapeutic%20effects%20of%20Nigella%20sativa%20against%20chronic%20lead%20acetate-induced%20reproductive%20dysfunction%20in%20male%20Sprague-Dawley%20rats.pdf Assi, Mohammed Abdulrazzaq and Mohd Noor, Mohd Hezmee and Abba, Yusuf and Rajion, Mohamed Ali and Haron, Abd. Wahid and Mohd Yusoff, Md Sabri (2017) Assessment of therapeutic effects of Nigella sativa against chronic lead acetate-induced reproductive dysfunction in male Sprague-Dawley rats. Comparative Clinical Pathology, 26 (1). 87 - 97. ISSN 1618-5641; ESSN: 1618-565X https://link.springer.com/content/pdf/10.1007%2Fs00580-016-2349-3.pdf 10.1007/s00580-016-2349-3
institution	UPM IR
collection	UPM IR
language	English
description	Lead acetate (LA) is a toxic compound that has detrimental effects on the male reproductive system, such as decreased testicular size and function, low androgen hormone concentration, and altered testicular histology. While Nigella sativa (NS) has been shown to possess a handful of therapeutic benefits, few studies have shown its effect on damage caused by lead acetate toxicity in the male reproductive system. In this study, 75 Sprague-Dawley rats were divided into 3 groups of 25 rats, which were further subdivided into 5 subgroups each. Group 1 (negative control) was given distilled water, group 2 (positive control (PC)) administered 10 mg/kg of lead acetate (LA) orally/daily, while groups 3 (T1), 4 (T2), and 5 (T3) were given LA 10 mg/kg and graded concentrations (100, 150, and 200 mg/kg) each of NS. One group each, comprising of 25 rats, was euthanized at days 30, 60, and 90 for collection of blood plasma, epididymis, and organ tissues fixed in 10 % buffered formalin at each time interval. The right caudal epididymis was homogenized and used for the determination of spermiogram. Plasma was used for the determination of testosterone (TS), follicle stimulating hormone (FSH), and luteinizing hormone (LH) and estradiol (EST) using radioimmunoassay kits. There was reduced number of spermatogenic cells and epididymal sperm reserves in the PC group in comparison to the treatment groups. The level of TS was lower (p < 0.05) in the PC group at 90 days, while FSH was lower (p < 0.05) in T3 at 30 days and LH was higher (p < 0.05) in T1 at 90 days. The concentration of EST was lower (p < 0.05) in the PC, T1, and T2 at all time points, while the T3 group had the higher EST concentration that was similar to the control group. There was a decreased level of superoxide dismutase (SOD) and total glutathione (GSH) in the PC group and an increased GSH level in the T3 group. Sperm concentration, viability, and motility were adversely affected by LA, while concurrent treatment with NS significantly (p < 0.05) improved these parameters. This study showed the detrimental effects of LA on spermatogenesis, TS levels, and antioxidant defenses; however, these adverse effects were alleviated by oral NS administration.
format	Article
author	Assi, Mohammed Abdulrazzaq Mohd Noor, Mohd Hezmee Abba, Yusuf Rajion, Mohamed Ali Haron, Abd. Wahid Mohd Yusoff, Md Sabri
spellingShingle	Assi, Mohammed Abdulrazzaq Mohd Noor, Mohd Hezmee Abba, Yusuf Rajion, Mohamed Ali Haron, Abd. Wahid Mohd Yusoff, Md Sabri Assessment of therapeutic effects of Nigella sativa against chronic lead acetate-induced reproductive dysfunction in male Sprague-Dawley rats
author_facet	Assi, Mohammed Abdulrazzaq Mohd Noor, Mohd Hezmee Abba, Yusuf Rajion, Mohamed Ali Haron, Abd. Wahid Mohd Yusoff, Md Sabri
author_sort	Assi, Mohammed Abdulrazzaq
title	Assessment of therapeutic effects of Nigella sativa against chronic lead acetate-induced reproductive dysfunction in male Sprague-Dawley rats
title_short	Assessment of therapeutic effects of Nigella sativa against chronic lead acetate-induced reproductive dysfunction in male Sprague-Dawley rats
title_full	Assessment of therapeutic effects of Nigella sativa against chronic lead acetate-induced reproductive dysfunction in male Sprague-Dawley rats
title_fullStr	Assessment of therapeutic effects of Nigella sativa against chronic lead acetate-induced reproductive dysfunction in male Sprague-Dawley rats
title_full_unstemmed	Assessment of therapeutic effects of Nigella sativa against chronic lead acetate-induced reproductive dysfunction in male Sprague-Dawley rats
title_sort	assessment of therapeutic effects of nigella sativa against chronic lead acetate-induced reproductive dysfunction in male sprague-dawley rats
publisher	Springer
publishDate	2017
url	http://psasir.upm.edu.my/id/eprint/60950/1/Assessment%20of%20therapeutic%20effects%20of%20Nigella%20sativa%20against%20chronic%20lead%20acetate-induced%20reproductive%20dysfunction%20in%20male%20Sprague-Dawley%20rats.pdf
_version_	1782756213283356672
score	12.935284

Assessment of therapeutic effects of Nigella sativa against chronic lead acetate-induced reproductive dysfunction in male Sprague-Dawley rats

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