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Characterization of potential probionts from blue swimming crab Portunus pelagicus and its antagonistic activity against Vibrio harveyi

The aim of this study was to isolate local bacteria as potential probiont in controlling the growth of pathogenic Vibrio harveyi. Eleven potential bacteria were successfully isolated from the haemolymph of four healthy Portunus pelagicus. All of the isolates were identified as Bacillus amyloliquefac...

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Main Authors: Abdul Rahman, Noor Azrin, Esa, Yuzine, Md Yasin, Ina Salwany, R., Suzana, Masduki, Nur Fathiah, Abd Karim, Murni Marlina
Format: Article
Language:English
Published: International Network for Natural Sciences 2017
Online Access:http://psasir.upm.edu.my/id/eprint/61099/1/Characterization%20of%20potential%20probionts%20from%20blue%20swimming%20crab%20Portunus%20pelagicus%20and%20its%20antagonistic%20activity%20against%20Vibrio%20harveyi.pdf
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spelling oai:psasir.upm.edu.my:61099 http://psasir.upm.edu.my/id/eprint/61099/ Characterization of potential probionts from blue swimming crab Portunus pelagicus and its antagonistic activity against Vibrio harveyi Abdul Rahman, Noor Azrin Esa, Yuzine Md Yasin, Ina Salwany R., Suzana Masduki, Nur Fathiah Abd Karim, Murni Marlina The aim of this study was to isolate local bacteria as potential probiont in controlling the growth of pathogenic Vibrio harveyi. Eleven potential bacteria were successfully isolated from the haemolymph of four healthy Portunus pelagicus. All of the isolates were identified as Bacillus amyloliquefaciens by series of biochemical test using triple sugar ion test, oxidase and catalase test, followed by Internal Transcribe Spacer (ITS) gene sequence analysis. This isolate was able to inhibit the growth of V. harveyi in in-vitro screening assay by using well diffusion assay with the strong antagonistic activity from 7 to 16 mm. The potential strains at 108 CFU/ml showed highest inhibition response towards V. harveyi after co-cultured for 48 hr. The isolates produced four major extracellular enzymes which were amylase, protease, gelatinase and lipase and able to form biofilm after 24 hr of cultured. Thus, B. amyloliquefaciens showed important characteristics as probiotic which worth to be carried out for in vivo challenge assay. International Network for Natural Sciences 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/61099/1/Characterization%20of%20potential%20probionts%20from%20blue%20swimming%20crab%20Portunus%20pelagicus%20and%20its%20antagonistic%20activity%20against%20Vibrio%20harveyi.pdf Abdul Rahman, Noor Azrin and Esa, Yuzine and Md Yasin, Ina Salwany and R., Suzana and Masduki, Nur Fathiah and Abd Karim, Murni Marlina (2017) Characterization of potential probionts from blue swimming crab Portunus pelagicus and its antagonistic activity against Vibrio harveyi. International Journal of Biosciences, 11 (4). 292 - 303. ISSN 2220-6655; ESSN: 2222-5234 http://www.innspub.net/wp-content/uploads/2017/11/IJB-Vol-11-No-4-p-292-303.pdf 10.12692/ijb/11.4.292-303
institution UPM IR
collection UPM IR
language English
description The aim of this study was to isolate local bacteria as potential probiont in controlling the growth of pathogenic Vibrio harveyi. Eleven potential bacteria were successfully isolated from the haemolymph of four healthy Portunus pelagicus. All of the isolates were identified as Bacillus amyloliquefaciens by series of biochemical test using triple sugar ion test, oxidase and catalase test, followed by Internal Transcribe Spacer (ITS) gene sequence analysis. This isolate was able to inhibit the growth of V. harveyi in in-vitro screening assay by using well diffusion assay with the strong antagonistic activity from 7 to 16 mm. The potential strains at 108 CFU/ml showed highest inhibition response towards V. harveyi after co-cultured for 48 hr. The isolates produced four major extracellular enzymes which were amylase, protease, gelatinase and lipase and able to form biofilm after 24 hr of cultured. Thus, B. amyloliquefaciens showed important characteristics as probiotic which worth to be carried out for in vivo challenge assay.
format Article
author Abdul Rahman, Noor Azrin
Esa, Yuzine
Md Yasin, Ina Salwany
R., Suzana
Masduki, Nur Fathiah
Abd Karim, Murni Marlina
spellingShingle Abdul Rahman, Noor Azrin
Esa, Yuzine
Md Yasin, Ina Salwany
R., Suzana
Masduki, Nur Fathiah
Abd Karim, Murni Marlina
Characterization of potential probionts from blue swimming crab Portunus pelagicus and its antagonistic activity against Vibrio harveyi
author_facet Abdul Rahman, Noor Azrin
Esa, Yuzine
Md Yasin, Ina Salwany
R., Suzana
Masduki, Nur Fathiah
Abd Karim, Murni Marlina
author_sort Abdul Rahman, Noor Azrin
title Characterization of potential probionts from blue swimming crab Portunus pelagicus and its antagonistic activity against Vibrio harveyi
title_short Characterization of potential probionts from blue swimming crab Portunus pelagicus and its antagonistic activity against Vibrio harveyi
title_full Characterization of potential probionts from blue swimming crab Portunus pelagicus and its antagonistic activity against Vibrio harveyi
title_fullStr Characterization of potential probionts from blue swimming crab Portunus pelagicus and its antagonistic activity against Vibrio harveyi
title_full_unstemmed Characterization of potential probionts from blue swimming crab Portunus pelagicus and its antagonistic activity against Vibrio harveyi
title_sort characterization of potential probionts from blue swimming crab portunus pelagicus and its antagonistic activity against vibrio harveyi
publisher International Network for Natural Sciences
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/61099/1/Characterization%20of%20potential%20probionts%20from%20blue%20swimming%20crab%20Portunus%20pelagicus%20and%20its%20antagonistic%20activity%20against%20Vibrio%20harveyi.pdf
_version_ 1819298084445749248
score 13.4562235

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