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Eco-friendly formulated zinc oxide nanoparticles: induction of cell cycle arrest and apoptosis in the MCF-7 cancer cell line

Green products have strong potential in the discovery and development of unique drugs. Zinc oxide nanoparticles (ZnO NPs) have been observed to have powerful cytotoxicity against cells that cause breast cancer. The present study aims to examine the cell cycle profile, status of cell death, and pathw...

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Main Authors: Moghaddam, Amin Boroumand, Moniri, Mona, Azizi, Susan, Abdul Rahim, Raha, Ariff, Arbakariya, Navaderi, Mohammad, Mohamad, Rosfarizan
Format: Article
Language:English
Published: MDPI AG 2017
Online Access:http://psasir.upm.edu.my/id/eprint/61533/1/Eco-friendly%20formulated%20zinc%20oxide%20nanoparticles%2C%20induction%20of%20cell%20cycle%20arrest%20and%20apoptosis%20in%20the%20MCF-7%20cancer%20cell%20line.pdf
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spelling oai:psasir.upm.edu.my:61533 http://psasir.upm.edu.my/id/eprint/61533/ Eco-friendly formulated zinc oxide nanoparticles: induction of cell cycle arrest and apoptosis in the MCF-7 cancer cell line Moghaddam, Amin Boroumand Moniri, Mona Azizi, Susan Abdul Rahim, Raha Ariff, Arbakariya Navaderi, Mohammad Mohamad, Rosfarizan Green products have strong potential in the discovery and development of unique drugs. Zinc oxide nanoparticles (ZnO NPs) have been observed to have powerful cytotoxicity against cells that cause breast cancer. The present study aims to examine the cell cycle profile, status of cell death, and pathways of apoptosis in breast cancer cells (MCF-7) treated with biosynthesized ZnO NPs. The anti-proliferative activity of ZnO NPs was determined using MTT assay. Cell cycle analysis and the mode of cell death were evaluated using a flow cytometry instrument. Quantitative real-time-PCR (qRT-PCR) was employed to investigate the expression of apoptosis in MCF-7 cells. ZnO NPs were cytotoxic to the MCF-7 cells in a dose-dependent manner. The 50% growth inhibition concentration (IC50) of ZnO NPs at 24 h was 121 µg/mL. Cell cycle analysis revealed that ZnO NPs induced sub-G1 phase (apoptosis), with values of 1.87% at 0 μg/mL (control), 71.49% at IC25, 98.91% at IC50, and 99.44% at IC75. Annexin V/propidium iodide (PI) flow cytometry analysis confirmed that ZnO NPs induce apoptosis in MCF-7 cells. The pro-apoptotic genes p53, p21, Bax, and JNK were upregulated, whereas anti-apoptotic genes Bcl-2, AKT1, and ERK1/2 were downregulated in a dose-dependent manner. The arrest and apoptosis of MCF-7 cells were induced by ZnO NPs through several signalling pathways. MDPI AG 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/61533/1/Eco-friendly%20formulated%20zinc%20oxide%20nanoparticles%2C%20induction%20of%20cell%20cycle%20arrest%20and%20apoptosis%20in%20the%20MCF-7%20cancer%20cell%20line.pdf Moghaddam, Amin Boroumand and Moniri, Mona and Azizi, Susan and Abdul Rahim, Raha and Ariff, Arbakariya and Navaderi, Mohammad and Mohamad, Rosfarizan (2017) Eco-friendly formulated zinc oxide nanoparticles: induction of cell cycle arrest and apoptosis in the MCF-7 cancer cell line. Genes, 8 (10). art. no. 281. pp. 1-15. ISSN 2073-4425 https://www.mdpi.com/2073-4425/8/10/281/htm 10.3390/genes8100281
institution UPM IR
collection UPM IR
language English
description Green products have strong potential in the discovery and development of unique drugs. Zinc oxide nanoparticles (ZnO NPs) have been observed to have powerful cytotoxicity against cells that cause breast cancer. The present study aims to examine the cell cycle profile, status of cell death, and pathways of apoptosis in breast cancer cells (MCF-7) treated with biosynthesized ZnO NPs. The anti-proliferative activity of ZnO NPs was determined using MTT assay. Cell cycle analysis and the mode of cell death were evaluated using a flow cytometry instrument. Quantitative real-time-PCR (qRT-PCR) was employed to investigate the expression of apoptosis in MCF-7 cells. ZnO NPs were cytotoxic to the MCF-7 cells in a dose-dependent manner. The 50% growth inhibition concentration (IC50) of ZnO NPs at 24 h was 121 µg/mL. Cell cycle analysis revealed that ZnO NPs induced sub-G1 phase (apoptosis), with values of 1.87% at 0 μg/mL (control), 71.49% at IC25, 98.91% at IC50, and 99.44% at IC75. Annexin V/propidium iodide (PI) flow cytometry analysis confirmed that ZnO NPs induce apoptosis in MCF-7 cells. The pro-apoptotic genes p53, p21, Bax, and JNK were upregulated, whereas anti-apoptotic genes Bcl-2, AKT1, and ERK1/2 were downregulated in a dose-dependent manner. The arrest and apoptosis of MCF-7 cells were induced by ZnO NPs through several signalling pathways.
format Article
author Moghaddam, Amin Boroumand
Moniri, Mona
Azizi, Susan
Abdul Rahim, Raha
Ariff, Arbakariya
Navaderi, Mohammad
Mohamad, Rosfarizan
spellingShingle Moghaddam, Amin Boroumand
Moniri, Mona
Azizi, Susan
Abdul Rahim, Raha
Ariff, Arbakariya
Navaderi, Mohammad
Mohamad, Rosfarizan
Eco-friendly formulated zinc oxide nanoparticles: induction of cell cycle arrest and apoptosis in the MCF-7 cancer cell line
author_facet Moghaddam, Amin Boroumand
Moniri, Mona
Azizi, Susan
Abdul Rahim, Raha
Ariff, Arbakariya
Navaderi, Mohammad
Mohamad, Rosfarizan
author_sort Moghaddam, Amin Boroumand
title Eco-friendly formulated zinc oxide nanoparticles: induction of cell cycle arrest and apoptosis in the MCF-7 cancer cell line
title_short Eco-friendly formulated zinc oxide nanoparticles: induction of cell cycle arrest and apoptosis in the MCF-7 cancer cell line
title_full Eco-friendly formulated zinc oxide nanoparticles: induction of cell cycle arrest and apoptosis in the MCF-7 cancer cell line
title_fullStr Eco-friendly formulated zinc oxide nanoparticles: induction of cell cycle arrest and apoptosis in the MCF-7 cancer cell line
title_full_unstemmed Eco-friendly formulated zinc oxide nanoparticles: induction of cell cycle arrest and apoptosis in the MCF-7 cancer cell line
title_sort eco-friendly formulated zinc oxide nanoparticles: induction of cell cycle arrest and apoptosis in the mcf-7 cancer cell line
publisher MDPI AG
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/61533/1/Eco-friendly%20formulated%20zinc%20oxide%20nanoparticles%2C%20induction%20of%20cell%20cycle%20arrest%20and%20apoptosis%20in%20the%20MCF-7%20cancer%20cell%20line.pdf
_version_ 1819298115978526720
score 13.4562235

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