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Effect of different concentrations of soybean lecithin and virgin coconut oil in Tris-based extender on the quality of chilled and frozen-thawed bull semen

Aim: The objective of this study was to evaluate the effects of different concentrations of soybean lecithin (SL) and virgin coconut oil (VCO) in Tris-based extender on chilled and frozen-thawed bull semen quality parameters. Materials and Methods: A total of 24 ejaculates were collected from four b...

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Main Authors: Abdelbagi, Tarig Abdelrouf Ahmedeltayeb, Yusoff, Rosnina, Haron, Wahid, Yimer, N., Goh, Y. M., Baiee, F. H., Mada, Khumran Armiya'u, Salman, H., Assi, M. A., Ebrahimi, M.
Format: Article
Language:English
Published: Veterinary World 2017
Online Access:http://psasir.upm.edu.my/id/eprint/61557/1/Effect%20of%20different%20concentrations%20of%20soybean%20lecithin%20and%20virgin%20coconut%20oil%20in%20Tris-based%20extender%20on%20the%20quality%20of%20chilled%20and%20frozen-thawed%20bull%20semen.pdf
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spelling oai:psasir.upm.edu.my:61557 http://psasir.upm.edu.my/id/eprint/61557/ Effect of different concentrations of soybean lecithin and virgin coconut oil in Tris-based extender on the quality of chilled and frozen-thawed bull semen Abdelbagi, Tarig Abdelrouf Ahmedeltayeb Yusoff, Rosnina Haron, Wahid Yimer, N. Goh, Y. M. Baiee, F. H. Mada, Khumran Armiya'u Salman, H. Assi, M. A. Ebrahimi, M. Aim: The objective of this study was to evaluate the effects of different concentrations of soybean lecithin (SL) and virgin coconut oil (VCO) in Tris-based extender on chilled and frozen-thawed bull semen quality parameters. Materials and Methods: A total of 24 ejaculates were collected from four bulls via an electroejaculator. Semen samples were diluted with 2% VCO in Tris-based extender which consists of various concentrations of SL (1, 1.25, 1.5, and 1.75%). A 20% egg yolk in Tris used as a positive control (C+). The diluted semen samples were divided into two fractions; one for chilling which were stored at 4°C for 24, 72, and 144 h before evaluated for semen quality parameters. The second fraction used for freezing was chilled for 3 h at 4°C, packed into 0.25 mL straws and then cryopreserved in liquid nitrogen. The samples were then evaluated after 7 and 14 days. Chilled and frozen semen samples were thawed at 37°C and assessed for general motility using computer-assisted semen analysis, viability, acrosome integrity and morphology (eosin-nigrosin stain), membrane integrity, and lipid peroxidation using thiobarbituric acid reaction test. Results: The results showed that all the quality parameters assessed were significantly (p<0.05) improved at 1.5% SL concentration in chilled semen. Treatment groups of 1, 1.25, 1.5, and 1.75% SL were higher in quality parameters than the control group (C+) in chilled semen. However, all the quality parameters in frozen-thawed semen were significantly higher in the C+ than the treated groups. Conclusion: In conclusion, supplementation of 1.5% SL in 2% VCO Tris-based extender enhanced the chilled bull semen. However, there was no marked improvement in the frozen-thawed quality parameters after treatment. Veterinary World 2017-06 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/61557/1/Effect%20of%20different%20concentrations%20of%20soybean%20lecithin%20and%20virgin%20coconut%20oil%20in%20Tris-based%20extender%20on%20the%20quality%20of%20chilled%20and%20frozen-thawed%20bull%20semen.pdf Abdelbagi, Tarig Abdelrouf Ahmedeltayeb and Yusoff, Rosnina and Haron, Wahid and Yimer, N. and Goh, Y. M. and Baiee, F. H. and Mada, Khumran Armiya'u and Salman, H. and Assi, M. A. and Ebrahimi, M. (2017) Effect of different concentrations of soybean lecithin and virgin coconut oil in Tris-based extender on the quality of chilled and frozen-thawed bull semen. Veterinary World, 10 (6). 672 - 678. ISSN 0972-8988; ESSN: 2231-0916 http://www.veterinaryworld.org/Vol.10/June-2017/17.html 10.14202/vetworld.2017.672-678
institution UPM IR
collection UPM IR
language English
description Aim: The objective of this study was to evaluate the effects of different concentrations of soybean lecithin (SL) and virgin coconut oil (VCO) in Tris-based extender on chilled and frozen-thawed bull semen quality parameters. Materials and Methods: A total of 24 ejaculates were collected from four bulls via an electroejaculator. Semen samples were diluted with 2% VCO in Tris-based extender which consists of various concentrations of SL (1, 1.25, 1.5, and 1.75%). A 20% egg yolk in Tris used as a positive control (C+). The diluted semen samples were divided into two fractions; one for chilling which were stored at 4°C for 24, 72, and 144 h before evaluated for semen quality parameters. The second fraction used for freezing was chilled for 3 h at 4°C, packed into 0.25 mL straws and then cryopreserved in liquid nitrogen. The samples were then evaluated after 7 and 14 days. Chilled and frozen semen samples were thawed at 37°C and assessed for general motility using computer-assisted semen analysis, viability, acrosome integrity and morphology (eosin-nigrosin stain), membrane integrity, and lipid peroxidation using thiobarbituric acid reaction test. Results: The results showed that all the quality parameters assessed were significantly (p<0.05) improved at 1.5% SL concentration in chilled semen. Treatment groups of 1, 1.25, 1.5, and 1.75% SL were higher in quality parameters than the control group (C+) in chilled semen. However, all the quality parameters in frozen-thawed semen were significantly higher in the C+ than the treated groups. Conclusion: In conclusion, supplementation of 1.5% SL in 2% VCO Tris-based extender enhanced the chilled bull semen. However, there was no marked improvement in the frozen-thawed quality parameters after treatment.
format Article
author Abdelbagi, Tarig Abdelrouf Ahmedeltayeb
Yusoff, Rosnina
Haron, Wahid
Yimer, N.
Goh, Y. M.
Baiee, F. H.
Mada, Khumran Armiya'u
Salman, H.
Assi, M. A.
Ebrahimi, M.
spellingShingle Abdelbagi, Tarig Abdelrouf Ahmedeltayeb
Yusoff, Rosnina
Haron, Wahid
Yimer, N.
Goh, Y. M.
Baiee, F. H.
Mada, Khumran Armiya'u
Salman, H.
Assi, M. A.
Ebrahimi, M.
Effect of different concentrations of soybean lecithin and virgin coconut oil in Tris-based extender on the quality of chilled and frozen-thawed bull semen
author_facet Abdelbagi, Tarig Abdelrouf Ahmedeltayeb
Yusoff, Rosnina
Haron, Wahid
Yimer, N.
Goh, Y. M.
Baiee, F. H.
Mada, Khumran Armiya'u
Salman, H.
Assi, M. A.
Ebrahimi, M.
author_sort Abdelbagi, Tarig Abdelrouf Ahmedeltayeb
title Effect of different concentrations of soybean lecithin and virgin coconut oil in Tris-based extender on the quality of chilled and frozen-thawed bull semen
title_short Effect of different concentrations of soybean lecithin and virgin coconut oil in Tris-based extender on the quality of chilled and frozen-thawed bull semen
title_full Effect of different concentrations of soybean lecithin and virgin coconut oil in Tris-based extender on the quality of chilled and frozen-thawed bull semen
title_fullStr Effect of different concentrations of soybean lecithin and virgin coconut oil in Tris-based extender on the quality of chilled and frozen-thawed bull semen
title_full_unstemmed Effect of different concentrations of soybean lecithin and virgin coconut oil in Tris-based extender on the quality of chilled and frozen-thawed bull semen
title_sort effect of different concentrations of soybean lecithin and virgin coconut oil in tris-based extender on the quality of chilled and frozen-thawed bull semen
publisher Veterinary World
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/61557/1/Effect%20of%20different%20concentrations%20of%20soybean%20lecithin%20and%20virgin%20coconut%20oil%20in%20Tris-based%20extender%20on%20the%20quality%20of%20chilled%20and%20frozen-thawed%20bull%20semen.pdf
_version_ 1819298117843943424
score 13.4562235

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