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Effect of encapsulant and cryoprotectant on the viability of probiotic Pediococcus acidilactici ATCC 8042 during freeze-drying and exposure to high acidity, bile salts and heat

Encapsulation and freeze drying are among the most preferable preservation methods due to their considerable effects on probiotics survival. The effects of different cryoprotectants on the viability of free cells during freeze drying and coating materials for encapsulation strategy have been investi...

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Asıl Yazarlar: Halim, Murni, Mohd Mustafa, Nur Anizawanie, Othman, Majdiah, Wasoh, Helmi, Kapri, Mohd Rizal, Ariff, Arbakariya
Materyal Türü: Makale
Dil:English
Baskı/Yayın Bilgisi: Elsevier 2017
Online Erişim:http://psasir.upm.edu.my/id/eprint/61565/1/Effect%20of%20encapsulant%20and%20cryoprotectant%20on%20the%20viability%20of%20probiotic%20Pediococcus%20acidilactici%20ATCC%208042%20during%20freeze-drying%20and%20exposure%20to%20high%20acidity%2C%20bile%20salts%20and%20heat.pdf
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spelling oai:psasir.upm.edu.my:61565 http://psasir.upm.edu.my/id/eprint/61565/ Effect of encapsulant and cryoprotectant on the viability of probiotic Pediococcus acidilactici ATCC 8042 during freeze-drying and exposure to high acidity, bile salts and heat Halim, Murni Mohd Mustafa, Nur Anizawanie Othman, Majdiah Wasoh, Helmi Kapri, Mohd Rizal Ariff, Arbakariya Encapsulation and freeze drying are among the most preferable preservation methods due to their considerable effects on probiotics survival. The effects of different cryoprotectants on the viability of free cells during freeze drying and coating materials for encapsulation strategy have been investigated by various researchers. However, information on the effect of cryoprotectants on the viability of the encapsulated probiotics during freeze-drying seems scarce. In this study Pediococcus acidilactici ATCC 8042 was encapsulated using calcium-alginate (ALGsm-FD) and calcium-alginate co-coating with chitosan (CHsm-FD) via extrusion technique. They were subsequently freeze dried using 10% (w/w) skim milk as cryoprotectant. Immediately after the freeze drying, ALGsm-FD showed the highest survival (15.77%) compared to the CHsm-FD (0.36%), free cells protected with skim milk (FCsm-FD) (11.02%) and both encapsulated and free cell samples without the addition of skim milk (0%). Co-coating the entrapped cells in alginate bead with chitosan was only accommodating the cells survival during bile solution (pH ¼ 8.25) treatment but not for freeze drying and gastric juice treatment (pH ¼ 1.55). In comparison, ALGsm-FD and CHsm-FD were found to be more heat tolerance than FCsm-FD. The findings indicated that the supplementation of cryoprotectant conferred an important protective effect to either encapsulated or free probiotic cells during freeze drying. Elsevier 2017-08 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/61565/1/Effect%20of%20encapsulant%20and%20cryoprotectant%20on%20the%20viability%20of%20probiotic%20Pediococcus%20acidilactici%20ATCC%208042%20during%20freeze-drying%20and%20exposure%20to%20high%20acidity%2C%20bile%20salts%20and%20heat.pdf Halim, Murni and Mohd Mustafa, Nur Anizawanie and Othman, Majdiah and Wasoh, Helmi and Kapri, Mohd Rizal and Ariff, Arbakariya (2017) Effect of encapsulant and cryoprotectant on the viability of probiotic Pediococcus acidilactici ATCC 8042 during freeze-drying and exposure to high acidity, bile salts and heat. LWT- Food Science and Technology, 81. 210 - 216. ISSN 0023-6438 https://www.sciencedirect.com/science/article/pii/S0023643817302232?via%3Dihub 10.1016/j.lwt.2017.04.009
institution UPM IR
collection UPM IR
language English
description Encapsulation and freeze drying are among the most preferable preservation methods due to their considerable effects on probiotics survival. The effects of different cryoprotectants on the viability of free cells during freeze drying and coating materials for encapsulation strategy have been investigated by various researchers. However, information on the effect of cryoprotectants on the viability of the encapsulated probiotics during freeze-drying seems scarce. In this study Pediococcus acidilactici ATCC 8042 was encapsulated using calcium-alginate (ALGsm-FD) and calcium-alginate co-coating with chitosan (CHsm-FD) via extrusion technique. They were subsequently freeze dried using 10% (w/w) skim milk as cryoprotectant. Immediately after the freeze drying, ALGsm-FD showed the highest survival (15.77%) compared to the CHsm-FD (0.36%), free cells protected with skim milk (FCsm-FD) (11.02%) and both encapsulated and free cell samples without the addition of skim milk (0%). Co-coating the entrapped cells in alginate bead with chitosan was only accommodating the cells survival during bile solution (pH ¼ 8.25) treatment but not for freeze drying and gastric juice treatment (pH ¼ 1.55). In comparison, ALGsm-FD and CHsm-FD were found to be more heat tolerance than FCsm-FD. The findings indicated that the supplementation of cryoprotectant conferred an important protective effect to either encapsulated or free probiotic cells during freeze drying.
format Article
author Halim, Murni
Mohd Mustafa, Nur Anizawanie
Othman, Majdiah
Wasoh, Helmi
Kapri, Mohd Rizal
Ariff, Arbakariya
spellingShingle Halim, Murni
Mohd Mustafa, Nur Anizawanie
Othman, Majdiah
Wasoh, Helmi
Kapri, Mohd Rizal
Ariff, Arbakariya
Effect of encapsulant and cryoprotectant on the viability of probiotic Pediococcus acidilactici ATCC 8042 during freeze-drying and exposure to high acidity, bile salts and heat
author_facet Halim, Murni
Mohd Mustafa, Nur Anizawanie
Othman, Majdiah
Wasoh, Helmi
Kapri, Mohd Rizal
Ariff, Arbakariya
author_sort Halim, Murni
title Effect of encapsulant and cryoprotectant on the viability of probiotic Pediococcus acidilactici ATCC 8042 during freeze-drying and exposure to high acidity, bile salts and heat
title_short Effect of encapsulant and cryoprotectant on the viability of probiotic Pediococcus acidilactici ATCC 8042 during freeze-drying and exposure to high acidity, bile salts and heat
title_full Effect of encapsulant and cryoprotectant on the viability of probiotic Pediococcus acidilactici ATCC 8042 during freeze-drying and exposure to high acidity, bile salts and heat
title_fullStr Effect of encapsulant and cryoprotectant on the viability of probiotic Pediococcus acidilactici ATCC 8042 during freeze-drying and exposure to high acidity, bile salts and heat
title_full_unstemmed Effect of encapsulant and cryoprotectant on the viability of probiotic Pediococcus acidilactici ATCC 8042 during freeze-drying and exposure to high acidity, bile salts and heat
title_sort effect of encapsulant and cryoprotectant on the viability of probiotic pediococcus acidilactici atcc 8042 during freeze-drying and exposure to high acidity, bile salts and heat
publisher Elsevier
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/61565/1/Effect%20of%20encapsulant%20and%20cryoprotectant%20on%20the%20viability%20of%20probiotic%20Pediococcus%20acidilactici%20ATCC%208042%20during%20freeze-drying%20and%20exposure%20to%20high%20acidity%2C%20bile%20salts%20and%20heat.pdf
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score 13.4562235

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