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Evaluation of the cytotoxic effect of ellagic acid nanocomposite in lung cancer A549 cell line and RAW 264.9 cells

Ellagic acid nanocomposite (EAN) was prepared by direct reaction zinc oxide (ZnO) with ellagic acid (EA). The cytotoxicity of nanocomposite was examined on the A549 and RAW 264.9 cells using total protein content and MTT assays, respectively. It was found that IC50 values for A549 and RAW 264.9 cell...

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Main Authors: Hussein Al-Ali, Samer Hasan Ahmad, Al-Qubaisi, Mothanna Sadiq Obaid, Abdullah, Rasedee @ Mat, Hussein, Mohd Zobir
Format: Article
Language:English
Published: American Sientific Publishers 2017
Online Access:http://psasir.upm.edu.my/id/eprint/61795/1/Evaluation%20of%20the%20cytotoxic%20effect%20of%20ellagic%20acid%20nanocomposite%20in%20lung%20cancer%20A549%20cell%20line%20and%20RAW%20264.9%20cells.pdf
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spelling oai:psasir.upm.edu.my:61795 http://psasir.upm.edu.my/id/eprint/61795/ Evaluation of the cytotoxic effect of ellagic acid nanocomposite in lung cancer A549 cell line and RAW 264.9 cells Hussein Al-Ali, Samer Hasan Ahmad Al-Qubaisi, Mothanna Sadiq Obaid Abdullah, Rasedee @ Mat Hussein, Mohd Zobir Ellagic acid nanocomposite (EAN) was prepared by direct reaction zinc oxide (ZnO) with ellagic acid (EA). The cytotoxicity of nanocomposite was examined on the A549 and RAW 264.9 cells using total protein content and MTT assays, respectively. It was found that IC50 values for A549 and RAW 264.9 cells treated with EAN was 72.3 and 24.8 μg/ml, respectively. The amount of Bcl-2, Bax and p53 proteins as well as cytochrome C in the lung cancer A549 cells was determined to clarify the mechanism of action of nanocomposite in the killing of cells. The results show EAN can increase the Bax and p53 and cytochrome C for 42.2 and 11.5% at 12.5 μg/ml concentration, respectively. At same time, the nanocomposite can decrease the Bcl-2 protein 15.4% at above concentration. A Caspase-3 and caspase-9 result shows the apoptosis mechanism for killing cancer cells. American Sientific Publishers 2017-12 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/61795/1/Evaluation%20of%20the%20cytotoxic%20effect%20of%20ellagic%20acid%20nanocomposite%20in%20lung%20cancer%20A549%20cell%20line%20and%20RAW%20264.9%20cells.pdf Hussein Al-Ali, Samer Hasan Ahmad and Al-Qubaisi, Mothanna Sadiq Obaid and Abdullah, Rasedee @ Mat and Hussein, Mohd Zobir (2017) Evaluation of the cytotoxic effect of ellagic acid nanocomposite in lung cancer A549 cell line and RAW 264.9 cells. Journal of Bionanoscience, 11 (6). 578 - 583. ISSN 1557-7910; ESSN: 1557-7929 https://www.ingentaconnect.com/contentone/asp/jobn/2017/00000011/00000006/art00015 10.1166/jbns.2017.1473
institution UPM IR
collection UPM IR
language English
description Ellagic acid nanocomposite (EAN) was prepared by direct reaction zinc oxide (ZnO) with ellagic acid (EA). The cytotoxicity of nanocomposite was examined on the A549 and RAW 264.9 cells using total protein content and MTT assays, respectively. It was found that IC50 values for A549 and RAW 264.9 cells treated with EAN was 72.3 and 24.8 μg/ml, respectively. The amount of Bcl-2, Bax and p53 proteins as well as cytochrome C in the lung cancer A549 cells was determined to clarify the mechanism of action of nanocomposite in the killing of cells. The results show EAN can increase the Bax and p53 and cytochrome C for 42.2 and 11.5% at 12.5 μg/ml concentration, respectively. At same time, the nanocomposite can decrease the Bcl-2 protein 15.4% at above concentration. A Caspase-3 and caspase-9 result shows the apoptosis mechanism for killing cancer cells.
format Article
author Hussein Al-Ali, Samer Hasan Ahmad
Al-Qubaisi, Mothanna Sadiq Obaid
Abdullah, Rasedee @ Mat
Hussein, Mohd Zobir
spellingShingle Hussein Al-Ali, Samer Hasan Ahmad
Al-Qubaisi, Mothanna Sadiq Obaid
Abdullah, Rasedee @ Mat
Hussein, Mohd Zobir
Evaluation of the cytotoxic effect of ellagic acid nanocomposite in lung cancer A549 cell line and RAW 264.9 cells
author_facet Hussein Al-Ali, Samer Hasan Ahmad
Al-Qubaisi, Mothanna Sadiq Obaid
Abdullah, Rasedee @ Mat
Hussein, Mohd Zobir
author_sort Hussein Al-Ali, Samer Hasan Ahmad
title Evaluation of the cytotoxic effect of ellagic acid nanocomposite in lung cancer A549 cell line and RAW 264.9 cells
title_short Evaluation of the cytotoxic effect of ellagic acid nanocomposite in lung cancer A549 cell line and RAW 264.9 cells
title_full Evaluation of the cytotoxic effect of ellagic acid nanocomposite in lung cancer A549 cell line and RAW 264.9 cells
title_fullStr Evaluation of the cytotoxic effect of ellagic acid nanocomposite in lung cancer A549 cell line and RAW 264.9 cells
title_full_unstemmed Evaluation of the cytotoxic effect of ellagic acid nanocomposite in lung cancer A549 cell line and RAW 264.9 cells
title_sort evaluation of the cytotoxic effect of ellagic acid nanocomposite in lung cancer a549 cell line and raw 264.9 cells
publisher American Sientific Publishers
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/61795/1/Evaluation%20of%20the%20cytotoxic%20effect%20of%20ellagic%20acid%20nanocomposite%20in%20lung%20cancer%20A549%20cell%20line%20and%20RAW%20264.9%20cells.pdf
_version_ 1819298135858479104
score 13.4562235

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