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GC-MS based metabolite profiling, antioxidant and antimicrobial properties of different solvent extracts of Malaysian Plectranthus amboinicus leaves

This study evaluates the phytochemistry, antioxidant, and antimicrobial effects of Plectranthus amboinicus leaves extracted in different solvents. The methanol extract contained the highest total phenolic ( mg GAE/g) and flavonoid contents ( mg RE/g) and exhibited the highest DPPH scavenging activit...

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Main Authors: Swamy, Mallappa Kumara, Arumugam, Greetha, Kaur, Ravinder, Ghasemzadeh, Ali, Mohd. Yusoff, Mazina, Sinniah, Uma Rani
Format: Article
Language:English
Published: Hindawi Publishing Corporation 2017
Online Access:http://psasir.upm.edu.my/id/eprint/61938/1/GC-MS%20based%20metabolite%20profiling.pdf
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spelling oai:psasir.upm.edu.my:61938 http://psasir.upm.edu.my/id/eprint/61938/ GC-MS based metabolite profiling, antioxidant and antimicrobial properties of different solvent extracts of Malaysian Plectranthus amboinicus leaves Swamy, Mallappa Kumara Arumugam, Greetha Kaur, Ravinder Ghasemzadeh, Ali Mohd. Yusoff, Mazina Sinniah, Uma Rani This study evaluates the phytochemistry, antioxidant, and antimicrobial effects of Plectranthus amboinicus leaves extracted in different solvents. The methanol extract contained the highest total phenolic ( mg GAE/g) and flavonoid contents ( mg RE/g) and exhibited the highest DPPH scavenging activity (90.13 ± 3.32%) followed by the acetone extract (80.23 ± 3.26%) at 500 μg/mL concentration. Similarly, the highest ferric ion reduction potential ( μM of Fe (II)/g dry weight) was exhibited by the methanol extract followed by the acetone extract ( μM of Fe (II)/g dry weight). The methanol extract showed greater antimicrobial activity against all the tested pathogens (Bacillus subtilis, Methicillin-resistant Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Candida albicans). However, both hexane and acetone extracts failed to inhibit E. coli. S. aureus and C. albicans were more susceptible to all the extracts. Further, GC-MS analysis confirmed the occurrence of a total 46 phytocompounds in different solvent extracts. Some of the major compounds included carvacrol (37.7%), tetracontane (16.6%), squalene (15.6%), tetrapentacontane (13.7%), and Phytol (12.9%). In conclusion, extraction solvents influenced the recovery of phytocompounds and the highest pharmacological activities of the methanol extract could be correlated to the presence of additional bioactive compounds. Hindawi Publishing Corporation 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/61938/1/GC-MS%20based%20metabolite%20profiling.pdf Swamy, Mallappa Kumara and Arumugam, Greetha and Kaur, Ravinder and Ghasemzadeh, Ali and Mohd. Yusoff, Mazina and Sinniah, Uma Rani (2017) GC-MS based metabolite profiling, antioxidant and antimicrobial properties of different solvent extracts of Malaysian Plectranthus amboinicus leaves. Evidence-Based Complementary and Alternative Medicine, 2017. art. no. 1517683. pp. 1-10. ISSN 1741-427X; ESSN: 1741-4288 https://www.hindawi.com/journals/ecam/2017/1517683/ 10.1155/2017/1517683
institution UPM IR
collection UPM IR
language English
description This study evaluates the phytochemistry, antioxidant, and antimicrobial effects of Plectranthus amboinicus leaves extracted in different solvents. The methanol extract contained the highest total phenolic ( mg GAE/g) and flavonoid contents ( mg RE/g) and exhibited the highest DPPH scavenging activity (90.13 ± 3.32%) followed by the acetone extract (80.23 ± 3.26%) at 500 μg/mL concentration. Similarly, the highest ferric ion reduction potential ( μM of Fe (II)/g dry weight) was exhibited by the methanol extract followed by the acetone extract ( μM of Fe (II)/g dry weight). The methanol extract showed greater antimicrobial activity against all the tested pathogens (Bacillus subtilis, Methicillin-resistant Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Candida albicans). However, both hexane and acetone extracts failed to inhibit E. coli. S. aureus and C. albicans were more susceptible to all the extracts. Further, GC-MS analysis confirmed the occurrence of a total 46 phytocompounds in different solvent extracts. Some of the major compounds included carvacrol (37.7%), tetracontane (16.6%), squalene (15.6%), tetrapentacontane (13.7%), and Phytol (12.9%). In conclusion, extraction solvents influenced the recovery of phytocompounds and the highest pharmacological activities of the methanol extract could be correlated to the presence of additional bioactive compounds.
format Article
author Swamy, Mallappa Kumara
Arumugam, Greetha
Kaur, Ravinder
Ghasemzadeh, Ali
Mohd. Yusoff, Mazina
Sinniah, Uma Rani
spellingShingle Swamy, Mallappa Kumara
Arumugam, Greetha
Kaur, Ravinder
Ghasemzadeh, Ali
Mohd. Yusoff, Mazina
Sinniah, Uma Rani
GC-MS based metabolite profiling, antioxidant and antimicrobial properties of different solvent extracts of Malaysian Plectranthus amboinicus leaves
author_facet Swamy, Mallappa Kumara
Arumugam, Greetha
Kaur, Ravinder
Ghasemzadeh, Ali
Mohd. Yusoff, Mazina
Sinniah, Uma Rani
author_sort Swamy, Mallappa Kumara
title GC-MS based metabolite profiling, antioxidant and antimicrobial properties of different solvent extracts of Malaysian Plectranthus amboinicus leaves
title_short GC-MS based metabolite profiling, antioxidant and antimicrobial properties of different solvent extracts of Malaysian Plectranthus amboinicus leaves
title_full GC-MS based metabolite profiling, antioxidant and antimicrobial properties of different solvent extracts of Malaysian Plectranthus amboinicus leaves
title_fullStr GC-MS based metabolite profiling, antioxidant and antimicrobial properties of different solvent extracts of Malaysian Plectranthus amboinicus leaves
title_full_unstemmed GC-MS based metabolite profiling, antioxidant and antimicrobial properties of different solvent extracts of Malaysian Plectranthus amboinicus leaves
title_sort gc-ms based metabolite profiling, antioxidant and antimicrobial properties of different solvent extracts of malaysian plectranthus amboinicus leaves
publisher Hindawi Publishing Corporation
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/61938/1/GC-MS%20based%20metabolite%20profiling.pdf
_version_ 1819298146546614272
score 13.4562235

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