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Human mesenchymal stem cells promote CD34þhematopoietic stemcell proliferation with preserved red blood cell differentiation capacity

Studies showed that co-transplantation of mesenchymal stem cells (MSCs) and cord blood-derived CD34+ hematopoietic stem cells (HSCs) offered greater therapeutic effects but little is known regarding the effects of human Wharton's jelly derived MSCs on HSC expansion and red blood cell (RBC) gene...

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Main Authors: Lau, Show Xuan, Leong, Yin Yee, Ng, Wai Hoe, Ng, Albert Wee Po, Ismail, Ida Shazrina, Mohd Yusoff, Narazah, Ramasamy, Rajesh, Tan, Jun Jie
Format: Article
Language:English
Published: International Federation for Cell Biology 2017
Online Access:http://psasir.upm.edu.my/id/eprint/62053/1/Human%20mesenchymal%20stem%20cells%20promote.pdf
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spelling oai:psasir.upm.edu.my:62053 http://psasir.upm.edu.my/id/eprint/62053/ Human mesenchymal stem cells promote CD34þhematopoietic stemcell proliferation with preserved red blood cell differentiation capacity Lau, Show Xuan Leong, Yin Yee Ng, Wai Hoe Ng, Albert Wee Po Ismail, Ida Shazrina Mohd Yusoff, Narazah Ramasamy, Rajesh Tan, Jun Jie Studies showed that co-transplantation of mesenchymal stem cells (MSCs) and cord blood-derived CD34+ hematopoietic stem cells (HSCs) offered greater therapeutic effects but little is known regarding the effects of human Wharton's jelly derived MSCs on HSC expansion and red blood cell (RBC) generation in vitro. This study aimed to investigate the effects of MSCs on HSC expansion and differentiation. HSCs were co-cultured with MSCs or with 10% MSCs-derived conditioned medium, with HSCs cultured under standard medium served as a control. Cell expansion rates, number of mononuclear cell post-expansion and number of enucleated cells post-differentiation were evaluated. HSCs showed superior proliferation in the presence of MSC with mean expansion rate of 3.5 × 108  ± 1.8 × 107 after day 7 compared to the conditioned medium and the control group (8.9 × 107  ± 1.1 × 108 and 7.0 × 107  ± 3.3 × 106 respectively, P < 0.001). Although no significant differences in RBC differentiation were observed between groups at passage IV, the number of enucleated cell was greater compared to earlier passages, indicating successful RBC differentiation. Cord blood-derived CD34+ HSCs can be greatly expanded by co-culturing with MSCs without affecting the RBC differentiation capability, suggesting the importance of direct MSC-HSCs contact in HSC expansion and RBC differentiation. International Federation for Cell Biology 2017-06 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/62053/1/Human%20mesenchymal%20stem%20cells%20promote.pdf Lau, Show Xuan and Leong, Yin Yee and Ng, Wai Hoe and Ng, Albert Wee Po and Ismail, Ida Shazrina and Mohd Yusoff, Narazah and Ramasamy, Rajesh and Tan, Jun Jie (2017) Human mesenchymal stem cells promote CD34þhematopoietic stemcell proliferation with preserved red blood cell differentiation capacity. Cell Biology International, 41 (6). 697 - 704. ISSN 1065-6995; ESSN: 1095-8355 https://onlinelibrary.wiley.com/doi/full/10.1002/cbin.10774 10.1002/cbin.10774
institution UPM IR
collection UPM IR
language English
description Studies showed that co-transplantation of mesenchymal stem cells (MSCs) and cord blood-derived CD34+ hematopoietic stem cells (HSCs) offered greater therapeutic effects but little is known regarding the effects of human Wharton's jelly derived MSCs on HSC expansion and red blood cell (RBC) generation in vitro. This study aimed to investigate the effects of MSCs on HSC expansion and differentiation. HSCs were co-cultured with MSCs or with 10% MSCs-derived conditioned medium, with HSCs cultured under standard medium served as a control. Cell expansion rates, number of mononuclear cell post-expansion and number of enucleated cells post-differentiation were evaluated. HSCs showed superior proliferation in the presence of MSC with mean expansion rate of 3.5 × 108  ± 1.8 × 107 after day 7 compared to the conditioned medium and the control group (8.9 × 107  ± 1.1 × 108 and 7.0 × 107  ± 3.3 × 106 respectively, P < 0.001). Although no significant differences in RBC differentiation were observed between groups at passage IV, the number of enucleated cell was greater compared to earlier passages, indicating successful RBC differentiation. Cord blood-derived CD34+ HSCs can be greatly expanded by co-culturing with MSCs without affecting the RBC differentiation capability, suggesting the importance of direct MSC-HSCs contact in HSC expansion and RBC differentiation.
format Article
author Lau, Show Xuan
Leong, Yin Yee
Ng, Wai Hoe
Ng, Albert Wee Po
Ismail, Ida Shazrina
Mohd Yusoff, Narazah
Ramasamy, Rajesh
Tan, Jun Jie
spellingShingle Lau, Show Xuan
Leong, Yin Yee
Ng, Wai Hoe
Ng, Albert Wee Po
Ismail, Ida Shazrina
Mohd Yusoff, Narazah
Ramasamy, Rajesh
Tan, Jun Jie
Human mesenchymal stem cells promote CD34þhematopoietic stemcell proliferation with preserved red blood cell differentiation capacity
author_facet Lau, Show Xuan
Leong, Yin Yee
Ng, Wai Hoe
Ng, Albert Wee Po
Ismail, Ida Shazrina
Mohd Yusoff, Narazah
Ramasamy, Rajesh
Tan, Jun Jie
author_sort Lau, Show Xuan
title Human mesenchymal stem cells promote CD34þhematopoietic stemcell proliferation with preserved red blood cell differentiation capacity
title_short Human mesenchymal stem cells promote CD34þhematopoietic stemcell proliferation with preserved red blood cell differentiation capacity
title_full Human mesenchymal stem cells promote CD34þhematopoietic stemcell proliferation with preserved red blood cell differentiation capacity
title_fullStr Human mesenchymal stem cells promote CD34þhematopoietic stemcell proliferation with preserved red blood cell differentiation capacity
title_full_unstemmed Human mesenchymal stem cells promote CD34þhematopoietic stemcell proliferation with preserved red blood cell differentiation capacity
title_sort human mesenchymal stem cells promote cd34þhematopoietic stemcell proliferation with preserved red blood cell differentiation capacity
publisher International Federation for Cell Biology
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/62053/1/Human%20mesenchymal%20stem%20cells%20promote.pdf
_version_ 1819298156131647488
score 13.4562235

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