In vitro activity of Piper sarmentosum ethanol leaf extract against Toxoplasma gondii tachyzoites
Purpose: To evaluate the activity of the ethanol leaf extract of Piper sarmentosum against toxoplasmosis. Methods: An in vitro anti-Toxoplasma study was conducted using Vero cells as a host for T. gondii. Clindamycin used as the reference drug. Light microscopy technique was used to study the in sit...
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Pharmacotherapy Group, Faculty of Pharmacy, University of Benin
2017
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| Accés en línia: | http://psasir.upm.edu.my/id/eprint/62133/1/In%20vitro%20activity%20of%20Piper%20sarmentosum%20ethanol%20leaf%20extract%20against%20Toxoplasma%20gondii%20tachyzoites.pdf |
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oai:psasir.upm.edu.my:62133 http://psasir.upm.edu.my/id/eprint/62133/ In vitro activity of Piper sarmentosum ethanol leaf extract against Toxoplasma gondii tachyzoites Devanthran, Kumareswaran Unyah, Ngah Zasmy Abdul Majid, Roslaini Abdullah, Wan Omar Purpose: To evaluate the activity of the ethanol leaf extract of Piper sarmentosum against toxoplasmosis. Methods: An in vitro anti-Toxoplasma study was conducted using Vero cells as a host for T. gondii. Clindamycin used as the reference drug. Light microscopy technique was used to study the in situ antiparasitic activity of T. gondii. Non-toxic concentrations of the ethanol extract for Vero cells were determined by methyl thiazolyl tetrazolium (MTT) cell proliferation. The presence of Toxoplasma gondii was observed by Giemsa staining. Results: The results showed that significant (p < 0.05) anti-toxoplasma activity of the ethanol extract, though lower than that of clindamycin (control drug), was achieved, with half-maximal inhibitory concentration (IC50) of 12.4 and 7.2 μg/mL for the extract and reference drug, respectively. After 24 hours of exposure to the extract, the inoculated Vero cells showed lower parasitemia and no remarkable morphological changes. Conclusion: The findings demonstrate that the ethanol extract of P. sarmentosum leaves are active against toxoplasmosis in vitro. However, further studies are required to determine the therapeutic significance of these findings in vivo Pharmacotherapy Group, Faculty of Pharmacy, University of Benin 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/62133/1/In%20vitro%20activity%20of%20Piper%20sarmentosum%20ethanol%20leaf%20extract%20against%20Toxoplasma%20gondii%20tachyzoites.pdf Devanthran, Kumareswaran and Unyah, Ngah Zasmy and Abdul Majid, Roslaini and Abdullah, Wan Omar (2017) In vitro activity of Piper sarmentosum ethanol leaf extract against Toxoplasma gondii tachyzoites. Tropical Journal of Pharmaceutical Research, 16 (11). 2667 - 2673. ISSN 1596-5996; ESSN: 1596-9827 https://www.tjpr.org/home/abstract.php?id=1980&aTitle=In%20vitro%20activity%20of%20Piper%20sarmentosum%20ethanol%20leaf%20extract%20against%20Toxoplasma%20gondii%20tachyzoites 10.4314/tjpr.v16i11.14 |
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English |
| description |
Purpose: To evaluate the activity of the ethanol leaf extract of Piper sarmentosum against toxoplasmosis. Methods: An in vitro anti-Toxoplasma study was conducted using Vero cells as a host for T. gondii. Clindamycin used as the reference drug. Light microscopy technique was used to study the in situ antiparasitic activity of T. gondii. Non-toxic concentrations of the ethanol extract for Vero cells were determined by methyl thiazolyl tetrazolium (MTT) cell proliferation. The presence of Toxoplasma gondii was observed by Giemsa staining. Results: The results showed that significant (p < 0.05) anti-toxoplasma activity of the ethanol extract, though lower than that of clindamycin (control drug), was achieved, with half-maximal inhibitory concentration (IC50) of 12.4 and 7.2 μg/mL for the extract and reference drug, respectively. After 24 hours of exposure to the extract, the inoculated Vero cells showed lower parasitemia and no remarkable morphological changes. Conclusion: The findings demonstrate that the ethanol extract of P. sarmentosum leaves are active against toxoplasmosis in vitro. However, further studies are required to determine the therapeutic significance of these findings in vivo |
| format |
Article |
| author |
Devanthran, Kumareswaran Unyah, Ngah Zasmy Abdul Majid, Roslaini Abdullah, Wan Omar |
| spellingShingle |
Devanthran, Kumareswaran Unyah, Ngah Zasmy Abdul Majid, Roslaini Abdullah, Wan Omar In vitro activity of Piper sarmentosum ethanol leaf extract against Toxoplasma gondii tachyzoites |
| author_facet |
Devanthran, Kumareswaran Unyah, Ngah Zasmy Abdul Majid, Roslaini Abdullah, Wan Omar |
| author_sort |
Devanthran, Kumareswaran |
| title |
In vitro activity of Piper sarmentosum ethanol leaf extract against Toxoplasma gondii tachyzoites |
| title_short |
In vitro activity of Piper sarmentosum ethanol leaf extract against Toxoplasma gondii tachyzoites |
| title_full |
In vitro activity of Piper sarmentosum ethanol leaf extract against Toxoplasma gondii tachyzoites |
| title_fullStr |
In vitro activity of Piper sarmentosum ethanol leaf extract against Toxoplasma gondii tachyzoites |
| title_full_unstemmed |
In vitro activity of Piper sarmentosum ethanol leaf extract against Toxoplasma gondii tachyzoites |
| title_sort |
in vitro activity of piper sarmentosum ethanol leaf extract against toxoplasma gondii tachyzoites |
| publisher |
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin |
| publishDate |
2017 |
| url |
http://psasir.upm.edu.my/id/eprint/62133/1/In%20vitro%20activity%20of%20Piper%20sarmentosum%20ethanol%20leaf%20extract%20against%20Toxoplasma%20gondii%20tachyzoites.pdf |
| _version_ |
1819298162639110144 |
| score |
13.4562235 |