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Efficacy of an outer membrane protein of Pasteurella haemolytica A2, A7 or A9-enriched vaccine against intratracheal challenge exposure in sheep

The outer membrane proteins (OMP) were extracted from the P. haemolytica A2, A7 and A9 to determine their potential as immunogens and their capability for cross-protection. Sixty lambs of approximately 9 months old were divided into four main groups. Animals in Group 1 were vaccinated with 2 ml vacc...

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Main Authors: Mohd Yusoff, Md Sabri, Saad, Mohd Zamri, Mutalib, Abdul Rahim, Israf Ali, Daud Ahmad, N., Muniandy
Format: Article
Language:English
English
Published: 2000 Elsevier Science B.V. 2000
Online Access:http://psasir.upm.edu.my/id/eprint/6281/1/Efficacy%20of%20an%20outer%20membrane%20protein%20of%20Pasteurella%20haemolytica%20A2.pdf
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spelling oai:psasir.upm.edu.my:6281 http://psasir.upm.edu.my/id/eprint/6281/ Efficacy of an outer membrane protein of Pasteurella haemolytica A2, A7 or A9-enriched vaccine against intratracheal challenge exposure in sheep Mohd Yusoff, Md Sabri Saad, Mohd Zamri Mutalib, Abdul Rahim Israf Ali, Daud Ahmad N., Muniandy The outer membrane proteins (OMP) were extracted from the P. haemolytica A2, A7 and A9 to determine their potential as immunogens and their capability for cross-protection. Sixty lambs of approximately 9 months old were divided into four main groups. Animals in Group 1 were vaccinated with 2 ml vaccine containing 100 mg/ml of the outer membrane proteins of P. haemolytica A2. Animals in Group 2 were similarly vaccinated with the OMPs of P. haemolytica A7 while Group 3 with OMPs of P. haemolytica A9. Animals in Group 4 were unvaccinated control. During the course of the study, serum was collected to evaluate the antibody levels toward each OMP. There appeared to be good immune responses. However, high antibody levels did not necessarily result in good protection of the animals, particularly against cross-infection with P. haemolytica A9 in animals vaccinated with the OMPs of P. haemolytica A2. It seemed that the antibody responses were more speci®c toward the homologous challenge but generally did not cross-protect against heterologous serotype challenge. However, the OMPs of P. haemolytica A7 produced good in vivo cross-protection and excellent correlations when good antibody responses against all serotypes led to successful reductions of the extent of lung lesions following homologous and heterologous challenge exposures. Thus, the OMPs of P. haemolytica A7 was effective in protecting animals against homologous and heterologous infection by live P. haemolytica A2, A7 and A9. 2000 Elsevier Science B.V. 2000-04-04 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/6281/1/Efficacy%20of%20an%20outer%20membrane%20protein%20of%20Pasteurella%20haemolytica%20A2.pdf Mohd Yusoff, Md Sabri and Saad, Mohd Zamri and Mutalib, Abdul Rahim and Israf Ali, Daud Ahmad and N., Muniandy (2000) Efficacy of an outer membrane protein of Pasteurella haemolytica A2, A7 or A9-enriched vaccine against intratracheal challenge exposure in sheep. Veterinary Microbiology, 73. pp. 13-23. ISSN 0378-1135 http://dx.doi.org/10.1016/S0378-1135(99)00205-9 English
institution UPM IR
collection UPM IR
language English
English
description The outer membrane proteins (OMP) were extracted from the P. haemolytica A2, A7 and A9 to determine their potential as immunogens and their capability for cross-protection. Sixty lambs of approximately 9 months old were divided into four main groups. Animals in Group 1 were vaccinated with 2 ml vaccine containing 100 mg/ml of the outer membrane proteins of P. haemolytica A2. Animals in Group 2 were similarly vaccinated with the OMPs of P. haemolytica A7 while Group 3 with OMPs of P. haemolytica A9. Animals in Group 4 were unvaccinated control. During the course of the study, serum was collected to evaluate the antibody levels toward each OMP. There appeared to be good immune responses. However, high antibody levels did not necessarily result in good protection of the animals, particularly against cross-infection with P. haemolytica A9 in animals vaccinated with the OMPs of P. haemolytica A2. It seemed that the antibody responses were more speci®c toward the homologous challenge but generally did not cross-protect against heterologous serotype challenge. However, the OMPs of P. haemolytica A7 produced good in vivo cross-protection and excellent correlations when good antibody responses against all serotypes led to successful reductions of the extent of lung lesions following homologous and heterologous challenge exposures. Thus, the OMPs of P. haemolytica A7 was effective in protecting animals against homologous and heterologous infection by live P. haemolytica A2, A7 and A9.
format Article
author Mohd Yusoff, Md Sabri
Saad, Mohd Zamri
Mutalib, Abdul Rahim
Israf Ali, Daud Ahmad
N., Muniandy
spellingShingle Mohd Yusoff, Md Sabri
Saad, Mohd Zamri
Mutalib, Abdul Rahim
Israf Ali, Daud Ahmad
N., Muniandy
Efficacy of an outer membrane protein of Pasteurella haemolytica A2, A7 or A9-enriched vaccine against intratracheal challenge exposure in sheep
author_facet Mohd Yusoff, Md Sabri
Saad, Mohd Zamri
Mutalib, Abdul Rahim
Israf Ali, Daud Ahmad
N., Muniandy
author_sort Mohd Yusoff, Md Sabri
title Efficacy of an outer membrane protein of Pasteurella haemolytica A2, A7 or A9-enriched vaccine against intratracheal challenge exposure in sheep
title_short Efficacy of an outer membrane protein of Pasteurella haemolytica A2, A7 or A9-enriched vaccine against intratracheal challenge exposure in sheep
title_full Efficacy of an outer membrane protein of Pasteurella haemolytica A2, A7 or A9-enriched vaccine against intratracheal challenge exposure in sheep
title_fullStr Efficacy of an outer membrane protein of Pasteurella haemolytica A2, A7 or A9-enriched vaccine against intratracheal challenge exposure in sheep
title_full_unstemmed Efficacy of an outer membrane protein of Pasteurella haemolytica A2, A7 or A9-enriched vaccine against intratracheal challenge exposure in sheep
title_sort efficacy of an outer membrane protein of pasteurella haemolytica a2, a7 or a9-enriched vaccine against intratracheal challenge exposure in sheep
publisher 2000 Elsevier Science B.V.
publishDate 2000
url http://psasir.upm.edu.my/id/eprint/6281/1/Efficacy%20of%20an%20outer%20membrane%20protein%20of%20Pasteurella%20haemolytica%20A2.pdf
_version_ 1819293084517662720
score 13.4562235

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