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Reproductive hormonal variations and adenohypophyseal lesions in pre-pubertal buffalo heifers inoculated with Pasteurella multocida type B: 2 and its immunogens

BACKGROUND: Hemorrhagic septicemia is a fatal disease of cattle and buffaloes caused by P. multocida. Although the pathogenesis of the bacteria has been well established in literature, there is a paucity of information on the possible role of the bacteria and its immunogens; lipopolysaccharide (LPS...

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Prif Awduron: Abdullah, Faez Firdaus Jesse, Ibrahim, Hayder Hamzah, Abba, Yusuf, Lim, Eric Teik Chung, Marza, Ali Dhiaa, Mazlan, Mazlina, Saad, Mohd Zamri, Omar, Abdul Rahman, Abu Bakar @ Zakaria, Md Zuki, Saharee, Abdul Aziz, Haron, Abd Wahid, Mohd Lila, Mohd Azmi
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Iaith:English
Cyhoeddwyd: BioMed Central 2017
Mynediad Ar-lein:http://psasir.upm.edu.my/id/eprint/62960/1/Reproductive%20hormonal%20variations%20and%20adenohypophyseal%20lesions%20in%20pre-pubertal%20buffalo.pdf
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spelling oai:psasir.upm.edu.my:62960 http://psasir.upm.edu.my/id/eprint/62960/ Reproductive hormonal variations and adenohypophyseal lesions in pre-pubertal buffalo heifers inoculated with Pasteurella multocida type B: 2 and its immunogens Abdullah, Faez Firdaus Jesse Ibrahim, Hayder Hamzah Abba, Yusuf Lim, Eric Teik Chung Marza, Ali Dhiaa Mazlan, Mazlina Saad, Mohd Zamri Omar, Abdul Rahman Abu Bakar @ Zakaria, Md Zuki Saharee, Abdul Aziz Haron, Abd Wahid Mohd Lila, Mohd Azmi BACKGROUND: Hemorrhagic septicemia is a fatal disease of cattle and buffaloes caused by P. multocida. Although the pathogenesis of the bacteria has been well established in literature, there is a paucity of information on the possible role of the bacteria and its immunogens; lipopolysaccharide (LPS) and outer membrane proteins (OMPs) on the reproductive capacity of buffalo heifers. METHODS: In this study, twenty one healthy prepubertal female buffaloes aged 8 months were divided into seven groups of 3 buffaloes each (G1-G7). Group 1 (G1) served as the negative control group and were inoculated orally with 10 mL sterile Phosphate Buffer Saline (PBS), groups 2 (G2) and 3 (G3) were inoculated orally and subcutaneously with 10 mL of 1012 colony forming unit (cfu) of P.multocida type B: 2, while groups 4 (G4) and 5 (G5) received 10 mL of bacterial LPS orally and intravenously, respectively. Lastly, groups 6 (G6) and 7 (G7) were orally and subcutaneously inoculated with 10 mL of bacterial OMPs. Whole blood was collected in EDTA vials at stipulated time points (0, 2, 4, 6, 8, 10, 12, 24, 36, 48, 72, 120, 168, 216, 264, 312, 360, 408, 456 and 504 h), while tissue sections of the pituitary glands were collected and transported to the histopathology laboratory in 10% buffered formalin for processing and Hematoxylin and eosin staining. Plasma levels of luteinizing hormone (LH), follicle stimulating hormone (FSH), progesterone (PG), estradiol (EST) and gonadotrophin releasing hormone (GnRH) were determined. RESULTS: The histopathological lesions observed in the pituitary gland included hemorrhage, congestion, inflammatory cell infiltration, hydropic degeneration, necrosis and edema. These changes were higher (p < 0.05) in distribution and severity in G3, G6 and G7. Hormonal concentrations of LH, FSH, PG, EST and GnRH declined in all inoculation groups as time elapsed and were lower (p < 0.05) than that of the control group. CONCLUSION: Based on these findings, P.multocida B: 2 and its immunogens can be said to negatively affect the hypothalamic-pituitary-gonadal axis, resulting in decreased levels of reproductive hormones which may predispose to infertility in buffalo heifers. BioMed Central 2017-04-05 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/62960/1/Reproductive%20hormonal%20variations%20and%20adenohypophyseal%20lesions%20in%20pre-pubertal%20buffalo.pdf Abdullah, Faez Firdaus Jesse and Ibrahim, Hayder Hamzah and Abba, Yusuf and Lim, Eric Teik Chung and Marza, Ali Dhiaa and Mazlan, Mazlina and Saad, Mohd Zamri and Omar, Abdul Rahman and Abu Bakar @ Zakaria, Md Zuki and Saharee, Abdul Aziz and Haron, Abd Wahid and Mohd Lila, Mohd Azmi (2017) Reproductive hormonal variations and adenohypophyseal lesions in pre-pubertal buffalo heifers inoculated with Pasteurella multocida type B: 2 and its immunogens. BMC Veterinary Research, 13. pp. 1-10. ISSN 1746-6148 https://bmcvetres.biomedcentral.com/track/pdf/10.1186/s12917-017-1010-y 10.1186/s12917-017-1010-y
institution UPM IR
collection UPM IR
language English
description BACKGROUND: Hemorrhagic septicemia is a fatal disease of cattle and buffaloes caused by P. multocida. Although the pathogenesis of the bacteria has been well established in literature, there is a paucity of information on the possible role of the bacteria and its immunogens; lipopolysaccharide (LPS) and outer membrane proteins (OMPs) on the reproductive capacity of buffalo heifers. METHODS: In this study, twenty one healthy prepubertal female buffaloes aged 8 months were divided into seven groups of 3 buffaloes each (G1-G7). Group 1 (G1) served as the negative control group and were inoculated orally with 10 mL sterile Phosphate Buffer Saline (PBS), groups 2 (G2) and 3 (G3) were inoculated orally and subcutaneously with 10 mL of 1012 colony forming unit (cfu) of P.multocida type B: 2, while groups 4 (G4) and 5 (G5) received 10 mL of bacterial LPS orally and intravenously, respectively. Lastly, groups 6 (G6) and 7 (G7) were orally and subcutaneously inoculated with 10 mL of bacterial OMPs. Whole blood was collected in EDTA vials at stipulated time points (0, 2, 4, 6, 8, 10, 12, 24, 36, 48, 72, 120, 168, 216, 264, 312, 360, 408, 456 and 504 h), while tissue sections of the pituitary glands were collected and transported to the histopathology laboratory in 10% buffered formalin for processing and Hematoxylin and eosin staining. Plasma levels of luteinizing hormone (LH), follicle stimulating hormone (FSH), progesterone (PG), estradiol (EST) and gonadotrophin releasing hormone (GnRH) were determined. RESULTS: The histopathological lesions observed in the pituitary gland included hemorrhage, congestion, inflammatory cell infiltration, hydropic degeneration, necrosis and edema. These changes were higher (p < 0.05) in distribution and severity in G3, G6 and G7. Hormonal concentrations of LH, FSH, PG, EST and GnRH declined in all inoculation groups as time elapsed and were lower (p < 0.05) than that of the control group. CONCLUSION: Based on these findings, P.multocida B: 2 and its immunogens can be said to negatively affect the hypothalamic-pituitary-gonadal axis, resulting in decreased levels of reproductive hormones which may predispose to infertility in buffalo heifers.
format Article
author Abdullah, Faez Firdaus Jesse
Ibrahim, Hayder Hamzah
Abba, Yusuf
Lim, Eric Teik Chung
Marza, Ali Dhiaa
Mazlan, Mazlina
Saad, Mohd Zamri
Omar, Abdul Rahman
Abu Bakar @ Zakaria, Md Zuki
Saharee, Abdul Aziz
Haron, Abd Wahid
Mohd Lila, Mohd Azmi
spellingShingle Abdullah, Faez Firdaus Jesse
Ibrahim, Hayder Hamzah
Abba, Yusuf
Lim, Eric Teik Chung
Marza, Ali Dhiaa
Mazlan, Mazlina
Saad, Mohd Zamri
Omar, Abdul Rahman
Abu Bakar @ Zakaria, Md Zuki
Saharee, Abdul Aziz
Haron, Abd Wahid
Mohd Lila, Mohd Azmi
Reproductive hormonal variations and adenohypophyseal lesions in pre-pubertal buffalo heifers inoculated with Pasteurella multocida type B: 2 and its immunogens
author_facet Abdullah, Faez Firdaus Jesse
Ibrahim, Hayder Hamzah
Abba, Yusuf
Lim, Eric Teik Chung
Marza, Ali Dhiaa
Mazlan, Mazlina
Saad, Mohd Zamri
Omar, Abdul Rahman
Abu Bakar @ Zakaria, Md Zuki
Saharee, Abdul Aziz
Haron, Abd Wahid
Mohd Lila, Mohd Azmi
author_sort Abdullah, Faez Firdaus Jesse
title Reproductive hormonal variations and adenohypophyseal lesions in pre-pubertal buffalo heifers inoculated with Pasteurella multocida type B: 2 and its immunogens
title_short Reproductive hormonal variations and adenohypophyseal lesions in pre-pubertal buffalo heifers inoculated with Pasteurella multocida type B: 2 and its immunogens
title_full Reproductive hormonal variations and adenohypophyseal lesions in pre-pubertal buffalo heifers inoculated with Pasteurella multocida type B: 2 and its immunogens
title_fullStr Reproductive hormonal variations and adenohypophyseal lesions in pre-pubertal buffalo heifers inoculated with Pasteurella multocida type B: 2 and its immunogens
title_full_unstemmed Reproductive hormonal variations and adenohypophyseal lesions in pre-pubertal buffalo heifers inoculated with Pasteurella multocida type B: 2 and its immunogens
title_sort reproductive hormonal variations and adenohypophyseal lesions in pre-pubertal buffalo heifers inoculated with pasteurella multocida type b: 2 and its immunogens
publisher BioMed Central
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/62960/1/Reproductive%20hormonal%20variations%20and%20adenohypophyseal%20lesions%20in%20pre-pubertal%20buffalo.pdf
_version_ 1819298208364363776
score 13.4562235

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