Isolation of Neospora caninum from a calf in Malaysia
In order to attempt isolate the protozoan parasite Neospora caninum, an N. caninum seropositive pregnant Sahiwal Friesian cross heifer from a large-scale dairy farm in Malaysia was kept for observation until parturition at the Veterinary Research Institute, Ipoh. The heifer gave birth to a female ca...
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Elsevier
2004
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oai:psasir.upm.edu.my:6326 http://psasir.upm.edu.my/id/eprint/6326/ Isolation of Neospora caninum from a calf in Malaysia Cheah, Tong Soon Mattsson, Jens G. Mohd Zain, Zaini Abdullah Sani, Rehana Jakubek, Eva Britt Uggla, Arvid Panchadcharam, Chandrawathani In order to attempt isolate the protozoan parasite Neospora caninum, an N. caninum seropositive pregnant Sahiwal Friesian cross heifer from a large-scale dairy farm in Malaysia was kept for observation until parturition at the Veterinary Research Institute, Ipoh. The heifer gave birth to a female calf that was weak, underweight and unable to rise. Precolostral serum from the calf had an N. caninum indirect fluorescent antibody test titre of 1:3200. It died 12 h after birth and necropsy was performed. Brain homogenate from the calf was inoculated into 10 BALB/c mice that were kept for 3 months after which brain tissue from the mice was inoculated onto 24 h fresh monolayer Vero cell lines. The cell cultures were examined daily until growth of intracellular protozoa was observed. DNA of the organisms from the cell cultures was analyzed by PCR and DNA sequencing. DNA fragments of the expected size were amplified from the isolate using N. caninum-specific primers, and sequence analysis of ITS1 clearly identified the isolate as N. caninum. This is the first successful isolation of N. caninum from a bovine in Malaysia, and the isolate is designated Nc-MalB1. Elsevier 2004-12-15 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/6326/1/Isolation%20of%20Neospora%20caninum%20from%20a%20calf%20in%20Malaysia.pdf Cheah, Tong Soon and Mattsson, Jens G. and Mohd Zain, Zaini and Abdullah Sani, Rehana and Jakubek, Eva Britt and Uggla, Arvid and Panchadcharam, Chandrawathani (2004) Isolation of Neospora caninum from a calf in Malaysia. Veterinary Parasitology, 126 (3). pp. 263-269. ISSN 0304-4017 http://dx.doi.org/10.1016/j.vetpar.2004.08.012 10.1016/j.vetpar.2004.08.012 English |
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In order to attempt isolate the protozoan parasite Neospora caninum, an N. caninum seropositive pregnant Sahiwal Friesian cross heifer from a large-scale dairy farm in Malaysia was kept for observation until parturition at the Veterinary Research Institute, Ipoh. The heifer gave birth to a female calf that was weak, underweight and unable to rise. Precolostral serum from the calf had an N. caninum indirect fluorescent antibody test titre of 1:3200. It died 12 h after birth and necropsy was performed. Brain homogenate from the calf was inoculated into 10 BALB/c mice that were kept for 3 months after which brain tissue from the mice was inoculated onto 24 h fresh monolayer Vero cell lines. The cell cultures were examined daily until growth of intracellular protozoa was observed. DNA of the organisms from the cell cultures was analyzed by PCR and DNA sequencing. DNA fragments of the expected size were amplified from the isolate using N. caninum-specific primers, and sequence analysis of ITS1 clearly identified the isolate as N. caninum. This is the first successful isolation of N. caninum from a bovine in Malaysia, and the isolate is designated Nc-MalB1.
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| format |
Article |
| author |
Cheah, Tong Soon Mattsson, Jens G. Mohd Zain, Zaini Abdullah Sani, Rehana Jakubek, Eva Britt Uggla, Arvid Panchadcharam, Chandrawathani |
| spellingShingle |
Cheah, Tong Soon Mattsson, Jens G. Mohd Zain, Zaini Abdullah Sani, Rehana Jakubek, Eva Britt Uggla, Arvid Panchadcharam, Chandrawathani Isolation of Neospora caninum from a calf in Malaysia |
| author_facet |
Cheah, Tong Soon Mattsson, Jens G. Mohd Zain, Zaini Abdullah Sani, Rehana Jakubek, Eva Britt Uggla, Arvid Panchadcharam, Chandrawathani |
| author_sort |
Cheah, Tong Soon |
| title |
Isolation of Neospora caninum from a calf in Malaysia |
| title_short |
Isolation of Neospora caninum from a calf in Malaysia |
| title_full |
Isolation of Neospora caninum from a calf in Malaysia |
| title_fullStr |
Isolation of Neospora caninum from a calf in Malaysia |
| title_full_unstemmed |
Isolation of Neospora caninum from a calf in Malaysia |
| title_sort |
isolation of neospora caninum from a calf in malaysia |
| publisher |
Elsevier |
| publishDate |
2004 |
| url |
http://psasir.upm.edu.my/id/eprint/6326/1/Isolation%20of%20Neospora%20caninum%20from%20a%20calf%20in%20Malaysia.pdf |
| _version_ |
1819293089015005184 |
| score |
13.4562235 |