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Statistical optimization of hexavalent molybdenum reduction by Serratia sp. strain MIE2 using Central Composite Design (CCD)

The conversion of hexavalent molybdenum (Mo (VI)) to Mo-blue is a bioremediation techniquewhich reduces the toxicity of molybdenum to a less toxic form by bacteria. The aim of this studyis to determine the optimum conditions of significant parameters or variables that affect thereduction of Mo (VI)...

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Main Authors: Aziz, Nor Farahim, Halmi, Mohd Izuan Effendi, Wan Johari, Wan Lutfi
Format: Article
Language:English
Published: Hibiscus Publisher 2017
Online Access:http://psasir.upm.edu.my/id/eprint/63285/1/Statistical%20optimization%20of%20hexavalent%20molybdenum%20reduction%20by%20Serratia%20sp.%20strain%20MIE2%20using%20Central%20Composite%20Design%20%28CCD%29%20.pdf
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spelling oai:psasir.upm.edu.my:63285 http://psasir.upm.edu.my/id/eprint/63285/ Statistical optimization of hexavalent molybdenum reduction by Serratia sp. strain MIE2 using Central Composite Design (CCD) Aziz, Nor Farahim Halmi, Mohd Izuan Effendi Wan Johari, Wan Lutfi The conversion of hexavalent molybdenum (Mo (VI)) to Mo-blue is a bioremediation techniquewhich reduces the toxicity of molybdenum to a less toxic form by bacteria. The aim of this studyis to determine the optimum conditions of significant parameters or variables that affect thereduction of Mo (VI) to Mo-blue by the local isolate identified as Serratia sp. strain MIE2.Response Surface Methodology (RSM) was used in this study to optimize the reduction processusing Central Composite Design (CCD) as an optimization matrix. The optimum conditionspredicted by RSM using the desirability function for the reduction process were 20 mMmolybdate concentration, 3.95 mM phosphate, 6.25 pH and 25 g/L glucose and Mo-blueproduction occurred at the absorbance value of 20.5 at 865 nm. The validation of the predictedoptimum points showed the Mo-blue production occurred at the absorbance value of 21.85 witha deviation around 6.6 % from the RSM predicted value. Hibiscus Publisher 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/63285/1/Statistical%20optimization%20of%20hexavalent%20molybdenum%20reduction%20by%20Serratia%20sp.%20strain%20MIE2%20using%20Central%20Composite%20Design%20%28CCD%29%20.pdf Aziz, Nor Farahim and Halmi, Mohd Izuan Effendi and Wan Johari, Wan Lutfi (2017) Statistical optimization of hexavalent molybdenum reduction by Serratia sp. strain MIE2 using Central Composite Design (CCD). Journal of Biochemistry, Microbiology and Biotechnology, 5 (2). pp. 8-11. ISSN 2289-5779 https://journal.hibiscuspublisher.com/index.php/JOBIMB/article/view/341/349
institution UPM IR
collection UPM IR
language English
description The conversion of hexavalent molybdenum (Mo (VI)) to Mo-blue is a bioremediation techniquewhich reduces the toxicity of molybdenum to a less toxic form by bacteria. The aim of this studyis to determine the optimum conditions of significant parameters or variables that affect thereduction of Mo (VI) to Mo-blue by the local isolate identified as Serratia sp. strain MIE2.Response Surface Methodology (RSM) was used in this study to optimize the reduction processusing Central Composite Design (CCD) as an optimization matrix. The optimum conditionspredicted by RSM using the desirability function for the reduction process were 20 mMmolybdate concentration, 3.95 mM phosphate, 6.25 pH and 25 g/L glucose and Mo-blueproduction occurred at the absorbance value of 20.5 at 865 nm. The validation of the predictedoptimum points showed the Mo-blue production occurred at the absorbance value of 21.85 witha deviation around 6.6 % from the RSM predicted value.
format Article
author Aziz, Nor Farahim
Halmi, Mohd Izuan Effendi
Wan Johari, Wan Lutfi
spellingShingle Aziz, Nor Farahim
Halmi, Mohd Izuan Effendi
Wan Johari, Wan Lutfi
Statistical optimization of hexavalent molybdenum reduction by Serratia sp. strain MIE2 using Central Composite Design (CCD)
author_facet Aziz, Nor Farahim
Halmi, Mohd Izuan Effendi
Wan Johari, Wan Lutfi
author_sort Aziz, Nor Farahim
title Statistical optimization of hexavalent molybdenum reduction by Serratia sp. strain MIE2 using Central Composite Design (CCD)
title_short Statistical optimization of hexavalent molybdenum reduction by Serratia sp. strain MIE2 using Central Composite Design (CCD)
title_full Statistical optimization of hexavalent molybdenum reduction by Serratia sp. strain MIE2 using Central Composite Design (CCD)
title_fullStr Statistical optimization of hexavalent molybdenum reduction by Serratia sp. strain MIE2 using Central Composite Design (CCD)
title_full_unstemmed Statistical optimization of hexavalent molybdenum reduction by Serratia sp. strain MIE2 using Central Composite Design (CCD)
title_sort statistical optimization of hexavalent molybdenum reduction by serratia sp. strain mie2 using central composite design (ccd)
publisher Hibiscus Publisher
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/63285/1/Statistical%20optimization%20of%20hexavalent%20molybdenum%20reduction%20by%20Serratia%20sp.%20strain%20MIE2%20using%20Central%20Composite%20Design%20%28CCD%29%20.pdf
_version_ 1819298227772456960
score 13.4562235

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