Okładka

Determination of velogenic Newcastle disease virus strain tissue tropism and disease pathogenesis by application of immunoperoxidase staining andin situ PCR

Twenty four, 3-week-old specific pathogen free (SPF) chickens were infected with 10⁵ EID50/0.1 mL of velogenic Newcastle disease virus (vNDV) AF2240 isolate intra-nasally, and 15 SPF chickens were remained uninfected and acted as the control group. The chickens were sacrificed at various intervals t...

Szczegółowa specyfikacja

Zapisane w:
Opis bibliograficzny
Główni autorzy: Mahmoud, Hussein Abdallah Elawad, Bejo, Mohd Hair, Omar, Abdul Rahman, Arshad, Siti Suri, Ideris, Aini
Format: Conference or Workshop Item
Język:English
Wydane: Faculty of Veterinary Medicine, Universiti Putra Malaysia 2015
Dostęp online:http://psasir.upm.edu.my/id/eprint/65035/1/PA-36.pdf
Etykiety: Dodaj etykietę
Nie ma etykietki, Dołącz pierwszą etykiete!
id oai:psasir.upm.edu.my:65035
record_format eprints
spelling oai:psasir.upm.edu.my:65035 http://psasir.upm.edu.my/id/eprint/65035/ Determination of velogenic Newcastle disease virus strain tissue tropism and disease pathogenesis by application of immunoperoxidase staining andin situ PCR Mahmoud, Hussein Abdallah Elawad Bejo, Mohd Hair Omar, Abdul Rahman Arshad, Siti Suri Ideris, Aini Twenty four, 3-week-old specific pathogen free (SPF) chickens were infected with 10⁵ EID50/0.1 mL of velogenic Newcastle disease virus (vNDV) AF2240 isolate intra-nasally, and 15 SPF chickens were remained uninfected and acted as the control group. The chickens were sacrificed at various intervals through the trial. Samples of brain, trachea, lung, caecal tonsils, liver, kidney, spleen, heart, proventriculus, intestine, bursa of Fabricius and thymus were fixed in 10% buffered formalin, processed, and embedded in paraffin for immunoperoxidase staining (IPS) and in situ PCR examination. The study showed that following intranasal inoculation of vNDV in SPF chickens, the virus enter the trachea; respiratory tract. At the same time virus was also enter the caecal tonsils, intestine and bursa of Fabricus either through the direct contact from the intestinal lumen or primary viraemia. During the primary viraemia, the virus enter most of the organs examined, including the brain at 6 hours pi. It appears that secondary viraemia occurred as early as 12 hours pi when all samples of the organs examined were positive for the virus by in situ PCR. The main primary tissue tropism for vNDV is the trachea; tracheal or bronchial associated lymphoid tissues (BALT) followed by caecal tonsils; gastrointestinal tract associated lymphoid tissues (GALT). The virus also caused infection into the brain, the central nervous system. It was concluded that the in situ PCR and IPS techniques applied in the present study could determine the pathogenesis and tissue tropism of vNDV following intranasal inoculation of the virus in SPF chickens. Faculty of Veterinary Medicine, Universiti Putra Malaysia 2015 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/65035/1/PA-36.pdf Mahmoud, Hussein Abdallah Elawad and Bejo, Mohd Hair and Omar, Abdul Rahman and Arshad, Siti Suri and Ideris, Aini (2015) Determination of velogenic Newcastle disease virus strain tissue tropism and disease pathogenesis by application of immunoperoxidase staining andin situ PCR. In: World Veterinary Poultry Association (Malaysia Branch) and World's Poultry Science Association (Malaysia Branch) Scientific Conference 2015, 21-22 Sept. 2015, Kuala Lumpur Convention Centre, Kuala Lumpur, Malaysia. (pp. 182-184).
institution UPM IR
collection UPM IR
language English
description Twenty four, 3-week-old specific pathogen free (SPF) chickens were infected with 10⁵ EID50/0.1 mL of velogenic Newcastle disease virus (vNDV) AF2240 isolate intra-nasally, and 15 SPF chickens were remained uninfected and acted as the control group. The chickens were sacrificed at various intervals through the trial. Samples of brain, trachea, lung, caecal tonsils, liver, kidney, spleen, heart, proventriculus, intestine, bursa of Fabricius and thymus were fixed in 10% buffered formalin, processed, and embedded in paraffin for immunoperoxidase staining (IPS) and in situ PCR examination. The study showed that following intranasal inoculation of vNDV in SPF chickens, the virus enter the trachea; respiratory tract. At the same time virus was also enter the caecal tonsils, intestine and bursa of Fabricus either through the direct contact from the intestinal lumen or primary viraemia. During the primary viraemia, the virus enter most of the organs examined, including the brain at 6 hours pi. It appears that secondary viraemia occurred as early as 12 hours pi when all samples of the organs examined were positive for the virus by in situ PCR. The main primary tissue tropism for vNDV is the trachea; tracheal or bronchial associated lymphoid tissues (BALT) followed by caecal tonsils; gastrointestinal tract associated lymphoid tissues (GALT). The virus also caused infection into the brain, the central nervous system. It was concluded that the in situ PCR and IPS techniques applied in the present study could determine the pathogenesis and tissue tropism of vNDV following intranasal inoculation of the virus in SPF chickens.
format Conference or Workshop Item
author Mahmoud, Hussein Abdallah Elawad
Bejo, Mohd Hair
Omar, Abdul Rahman
Arshad, Siti Suri
Ideris, Aini
spellingShingle Mahmoud, Hussein Abdallah Elawad
Bejo, Mohd Hair
Omar, Abdul Rahman
Arshad, Siti Suri
Ideris, Aini
Determination of velogenic Newcastle disease virus strain tissue tropism and disease pathogenesis by application of immunoperoxidase staining andin situ PCR
author_facet Mahmoud, Hussein Abdallah Elawad
Bejo, Mohd Hair
Omar, Abdul Rahman
Arshad, Siti Suri
Ideris, Aini
author_sort Mahmoud, Hussein Abdallah Elawad
title Determination of velogenic Newcastle disease virus strain tissue tropism and disease pathogenesis by application of immunoperoxidase staining andin situ PCR
title_short Determination of velogenic Newcastle disease virus strain tissue tropism and disease pathogenesis by application of immunoperoxidase staining andin situ PCR
title_full Determination of velogenic Newcastle disease virus strain tissue tropism and disease pathogenesis by application of immunoperoxidase staining andin situ PCR
title_fullStr Determination of velogenic Newcastle disease virus strain tissue tropism and disease pathogenesis by application of immunoperoxidase staining andin situ PCR
title_full_unstemmed Determination of velogenic Newcastle disease virus strain tissue tropism and disease pathogenesis by application of immunoperoxidase staining andin situ PCR
title_sort determination of velogenic newcastle disease virus strain tissue tropism and disease pathogenesis by application of immunoperoxidase staining andin situ pcr
publisher Faculty of Veterinary Medicine, Universiti Putra Malaysia
publishDate 2015
url http://psasir.upm.edu.my/id/eprint/65035/1/PA-36.pdf
_version_ 1819298368796491776
score 13.4562235

Podobne zapisy