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Investigation of phytochemical components and bioautography of Garcinia mangostana L. methanol leaf extract

The aim of this work to identify chemical components of Garcinia mangostana leaves and test the antibacterial effect on Pseudomonas syringe pv. tomato and Xanthomonas oryzae pv. oryzae using bioautographic procedure. Phytochemical screening of methanolic extract showed that the leaf extract of G. ma...

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Main Authors: Alsultan, Qamar Mohammed Naji, Sijam, Kamaruzaman, Rashid, Tavga Sulaiman, Ahmad, Khairulmazmi, Awla, Hayman Kakakhan
Format: Article
Language:English
Published: SCIENCEDOMAIN International 2017
Online Access:http://psasir.upm.edu.my/id/eprint/65352/1/Investigation%20of%20phytochemical%20components%20and%20bioautography%20of%20Garcinia%20mangostana%20L.%20methanol%20leaf%20extract.pdf
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spelling oai:psasir.upm.edu.my:65352 http://psasir.upm.edu.my/id/eprint/65352/ Investigation of phytochemical components and bioautography of Garcinia mangostana L. methanol leaf extract Alsultan, Qamar Mohammed Naji Sijam, Kamaruzaman Rashid, Tavga Sulaiman Ahmad, Khairulmazmi Awla, Hayman Kakakhan The aim of this work to identify chemical components of Garcinia mangostana leaves and test the antibacterial effect on Pseudomonas syringe pv. tomato and Xanthomonas oryzae pv. oryzae using bioautographic procedure. Phytochemical screening of methanolic extract showed that the leaf extract of G. mangostana rich in alkaloids, Flavonoids, Saponins, Tannins, Phenol, Terpenoids, Anthraquinone and Cardiac glycosides. A simple bioautographic procedure, involving spraying suspensions of the bacteria on thin layer chromatography (TLC) plates developed in solvents of varying polarities was used to detect the number of antibacterial compounds present in the extract. This activity was indicated by white spots against a red background on the TLC plates after spraying with 5% TTC aqueous solution. P. syringae and X. oryzae were inhibited by the most compounds separated on the TLC plates from the extract. SCIENCEDOMAIN International 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/65352/1/Investigation%20of%20phytochemical%20components%20and%20bioautography%20of%20Garcinia%20mangostana%20L.%20methanol%20leaf%20extract.pdf Alsultan, Qamar Mohammed Naji and Sijam, Kamaruzaman and Rashid, Tavga Sulaiman and Ahmad, Khairulmazmi and Awla, Hayman Kakakhan (2017) Investigation of phytochemical components and bioautography of Garcinia mangostana L. methanol leaf extract. Journal of Experimental Agriculture International, 15 (3). art. no. JEAI.31398. pp. 1-7. ISSN 2457-0591 http://www.sciencedomain.org/abstract/17732 10.9734/JEAI/2017/31398
institution UPM IR
collection UPM IR
language English
description The aim of this work to identify chemical components of Garcinia mangostana leaves and test the antibacterial effect on Pseudomonas syringe pv. tomato and Xanthomonas oryzae pv. oryzae using bioautographic procedure. Phytochemical screening of methanolic extract showed that the leaf extract of G. mangostana rich in alkaloids, Flavonoids, Saponins, Tannins, Phenol, Terpenoids, Anthraquinone and Cardiac glycosides. A simple bioautographic procedure, involving spraying suspensions of the bacteria on thin layer chromatography (TLC) plates developed in solvents of varying polarities was used to detect the number of antibacterial compounds present in the extract. This activity was indicated by white spots against a red background on the TLC plates after spraying with 5% TTC aqueous solution. P. syringae and X. oryzae were inhibited by the most compounds separated on the TLC plates from the extract.
format Article
author Alsultan, Qamar Mohammed Naji
Sijam, Kamaruzaman
Rashid, Tavga Sulaiman
Ahmad, Khairulmazmi
Awla, Hayman Kakakhan
spellingShingle Alsultan, Qamar Mohammed Naji
Sijam, Kamaruzaman
Rashid, Tavga Sulaiman
Ahmad, Khairulmazmi
Awla, Hayman Kakakhan
Investigation of phytochemical components and bioautography of Garcinia mangostana L. methanol leaf extract
author_facet Alsultan, Qamar Mohammed Naji
Sijam, Kamaruzaman
Rashid, Tavga Sulaiman
Ahmad, Khairulmazmi
Awla, Hayman Kakakhan
author_sort Alsultan, Qamar Mohammed Naji
title Investigation of phytochemical components and bioautography of Garcinia mangostana L. methanol leaf extract
title_short Investigation of phytochemical components and bioautography of Garcinia mangostana L. methanol leaf extract
title_full Investigation of phytochemical components and bioautography of Garcinia mangostana L. methanol leaf extract
title_fullStr Investigation of phytochemical components and bioautography of Garcinia mangostana L. methanol leaf extract
title_full_unstemmed Investigation of phytochemical components and bioautography of Garcinia mangostana L. methanol leaf extract
title_sort investigation of phytochemical components and bioautography of garcinia mangostana l. methanol leaf extract
publisher SCIENCEDOMAIN International
publishDate 2017
url http://psasir.upm.edu.my/id/eprint/65352/1/Investigation%20of%20phytochemical%20components%20and%20bioautography%20of%20Garcinia%20mangostana%20L.%20methanol%20leaf%20extract.pdf
_version_ 1819298397586194432
score 13.4562235

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