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Characterisation of the probiotic qualities exhibited by lactobacilli strains isolated from the anogenital tract

Introduction: Lactobacilli are well-documented probiotics that exert health benefits on their host. They exhibit characteristics that make them potential alternative treatments to address the antimicrobial resistance conundrum and diseases. Their mechanism of action varies with strain and species. F...

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Main Authors: Choo, Sulin, Chew, Boon How, Mohd Jamil, Amilia Afzan, Chew, Shu Yih, Meleh, Hadiza Umar, Than, Leslie Thian Lung
Format: Article
Language:English
Published: Faculty of Medicine and Health Sciences, Universiti Putra Malaysia 2019
Online Access:http://psasir.upm.edu.my/id/eprint/67250/1/2019010811373906_MJMHS_Jan_2019.pdf
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spelling oai:psasir.upm.edu.my:67250 http://psasir.upm.edu.my/id/eprint/67250/ Characterisation of the probiotic qualities exhibited by lactobacilli strains isolated from the anogenital tract Choo, Sulin Chew, Boon How Mohd Jamil, Amilia Afzan Chew, Shu Yih Meleh, Hadiza Umar Than, Leslie Thian Lung Introduction: Lactobacilli are well-documented probiotics that exert health benefits on their host. They exhibit characteristics that make them potential alternative treatments to address the antimicrobial resistance conundrum and diseases. Their mechanism of action varies with strain and species. Five lactobacilli strains previously isolated from the anogenital region were subjected to several assessments highlighted in the FAO/WHO document, ‘Guidelines for the Evaluation of Probiotics in Food’ to determine its suitability as potential probiotics. Methods: The five lactobacilli strains were subcultured onto Man de Rogosa agar (MRS). Their ability to auto- and co-aggregate was determined spectrophotometrically. Simultaneously, the cell surface hydrophobic properties of these strains towards xylene and toluene were evaluated using the microbial adhesion to hydrocarbon (MATH) test. The lactobacilli strains were also tested for their ability to withstand acid, bile and spermicide to determine their level of tolerance. Results: Lact. reuteri 29A, L. delbrueckii 45E and L. reuteri 29B exhibited the highest degree of auto- and co-aggregation properties. These lactobacilli strains also demonstrated high cell surface hydrophobicity, with the exception of L. delbrueckii 45E. Further tests to evaluate the isolated lactobacilli tolerance identified L. reuteri 29B as the most tolerant strain towards low pH (pH 2.5 for 4 h), high bile concentration (0.5% for 4 h) and high spermicides concentration (up to 10%). Conclusion: Out of the five lactobacilli strains which possessed high antimicrobial activities, L. reuteri 29B portrayed the best probiotic qualities with good auto- and co-aggregation abilities and high tolerance against acid, bile and spermicide. Faculty of Medicine and Health Sciences, Universiti Putra Malaysia 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/67250/1/2019010811373906_MJMHS_Jan_2019.pdf Choo, Sulin and Chew, Boon How and Mohd Jamil, Amilia Afzan and Chew, Shu Yih and Meleh, Hadiza Umar and Than, Leslie Thian Lung (2019) Characterisation of the probiotic qualities exhibited by lactobacilli strains isolated from the anogenital tract. Malaysian Journal of Medicine and Health Sciences, 15 (1). pp. 37-45. ISSN 1675-8544; ESSN: 2636-9346 http://www.medic.upm.edu.my/upload/dokumen/2019010811373906_MJMHS_Jan_2019.pdf
institution UPM IR
collection UPM IR
language English
description Introduction: Lactobacilli are well-documented probiotics that exert health benefits on their host. They exhibit characteristics that make them potential alternative treatments to address the antimicrobial resistance conundrum and diseases. Their mechanism of action varies with strain and species. Five lactobacilli strains previously isolated from the anogenital region were subjected to several assessments highlighted in the FAO/WHO document, ‘Guidelines for the Evaluation of Probiotics in Food’ to determine its suitability as potential probiotics. Methods: The five lactobacilli strains were subcultured onto Man de Rogosa agar (MRS). Their ability to auto- and co-aggregate was determined spectrophotometrically. Simultaneously, the cell surface hydrophobic properties of these strains towards xylene and toluene were evaluated using the microbial adhesion to hydrocarbon (MATH) test. The lactobacilli strains were also tested for their ability to withstand acid, bile and spermicide to determine their level of tolerance. Results: Lact. reuteri 29A, L. delbrueckii 45E and L. reuteri 29B exhibited the highest degree of auto- and co-aggregation properties. These lactobacilli strains also demonstrated high cell surface hydrophobicity, with the exception of L. delbrueckii 45E. Further tests to evaluate the isolated lactobacilli tolerance identified L. reuteri 29B as the most tolerant strain towards low pH (pH 2.5 for 4 h), high bile concentration (0.5% for 4 h) and high spermicides concentration (up to 10%). Conclusion: Out of the five lactobacilli strains which possessed high antimicrobial activities, L. reuteri 29B portrayed the best probiotic qualities with good auto- and co-aggregation abilities and high tolerance against acid, bile and spermicide.
format Article
author Choo, Sulin
Chew, Boon How
Mohd Jamil, Amilia Afzan
Chew, Shu Yih
Meleh, Hadiza Umar
Than, Leslie Thian Lung
spellingShingle Choo, Sulin
Chew, Boon How
Mohd Jamil, Amilia Afzan
Chew, Shu Yih
Meleh, Hadiza Umar
Than, Leslie Thian Lung
Characterisation of the probiotic qualities exhibited by lactobacilli strains isolated from the anogenital tract
author_facet Choo, Sulin
Chew, Boon How
Mohd Jamil, Amilia Afzan
Chew, Shu Yih
Meleh, Hadiza Umar
Than, Leslie Thian Lung
author_sort Choo, Sulin
title Characterisation of the probiotic qualities exhibited by lactobacilli strains isolated from the anogenital tract
title_short Characterisation of the probiotic qualities exhibited by lactobacilli strains isolated from the anogenital tract
title_full Characterisation of the probiotic qualities exhibited by lactobacilli strains isolated from the anogenital tract
title_fullStr Characterisation of the probiotic qualities exhibited by lactobacilli strains isolated from the anogenital tract
title_full_unstemmed Characterisation of the probiotic qualities exhibited by lactobacilli strains isolated from the anogenital tract
title_sort characterisation of the probiotic qualities exhibited by lactobacilli strains isolated from the anogenital tract
publisher Faculty of Medicine and Health Sciences, Universiti Putra Malaysia
publishDate 2019
url http://psasir.upm.edu.my/id/eprint/67250/1/2019010811373906_MJMHS_Jan_2019.pdf
_version_ 1819298577084579840
score 13.4562235

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