Kapak Resmi

LCMS/MS metabolite profiling and analysis of acute toxicity effect of the ethanolic extract of Centella asiatica on zebrafish model

Centella asiatica or known as 'pegaga' in Malaysia, is a popular medicinal herb, which is being used as main ingredient or incorporated into various herbal products. Apart from efficacy, the chemical profile and potential toxic effect of the plant are two important aspects of concern towar...

Ful tanımlama

Kaydedildi:
Detaylı Bibliyografya
Asıl Yazarlar: Zakaria, Fauziahanim, Wan Ibrahim, Wan Norhamidah, Ismail, Intan Safinar, Ahmad, Hafandi, Manshoor, Nurhuda, Ismail, Nor Hadiani, Zainal, Zurina, Shaari, Khozirah
Materyal Türü: Makale
Dil:English
Baskı/Yayın Bilgisi: Universiti Putra Malaysia Press 2019
Online Erişim:http://psasir.upm.edu.my/id/eprint/68709/1/28.%20JST%201366-2018.pdf
Etiketler: Etiketle
Etiket eklenmemiş, İlk siz ekleyin!
id oai:psasir.upm.edu.my:68709
record_format eprints
spelling oai:psasir.upm.edu.my:68709 http://psasir.upm.edu.my/id/eprint/68709/ LCMS/MS metabolite profiling and analysis of acute toxicity effect of the ethanolic extract of Centella asiatica on zebrafish model Zakaria, Fauziahanim Wan Ibrahim, Wan Norhamidah Ismail, Intan Safinar Ahmad, Hafandi Manshoor, Nurhuda Ismail, Nor Hadiani Zainal, Zurina Shaari, Khozirah Centella asiatica or known as 'pegaga' in Malaysia, is a popular medicinal herb, which is being used as main ingredient or incorporated into various herbal products. Apart from efficacy, the chemical profile and potential toxic effect of the plant are two important aspects of concern towards ensuring product satisfaction and safety of consumers. This paper reports the qualitative and quantitative chemical analysis of the leaf ethanolic extract of C. asiatica using LCMS/MS. The acute toxiciy effect of the extract and selected marker chemical constituents were further analysed using a zebrafish model. Twenty constituents, were identified and the main chemical marker constituents of the plant viz asiaticoside, asiatic acid, and madecassic acid were further quantified. Asiaticoside was found to be present in higher concentration than the other marker constituents. Meanwhile in the acute toxicity test, the LD50 of the extract on the zebrafish model was determined to be 1250 mg/L while 100% mortality was observed at the highest test concentration of 2500 mg/L. However, acute toxicity evaluation on four marker triterpenoids of the herb, i.e asiatic acid, madecassic acid, asiaticoside and madecassoside, indicated them to be quite safe on the zebrafish model, with no mortality shown for test concentrations between 10 to 500 mg/kg BW. Universiti Putra Malaysia Press 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/68709/1/28.%20JST%201366-2018.pdf Zakaria, Fauziahanim and Wan Ibrahim, Wan Norhamidah and Ismail, Intan Safinar and Ahmad, Hafandi and Manshoor, Nurhuda and Ismail, Nor Hadiani and Zainal, Zurina and Shaari, Khozirah (2019) LCMS/MS metabolite profiling and analysis of acute toxicity effect of the ethanolic extract of Centella asiatica on zebrafish model. Pertanika Journal of Science & Technology, 27 (2). pp. 985-1003. ISSN 0128-7680; ESSN: 2231-8526 http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JST%20Vol.%2027%20(2)%20Apr.%202019/28.%20JST%201366-2018.pdf
institution UPM IR
collection UPM IR
language English
description Centella asiatica or known as 'pegaga' in Malaysia, is a popular medicinal herb, which is being used as main ingredient or incorporated into various herbal products. Apart from efficacy, the chemical profile and potential toxic effect of the plant are two important aspects of concern towards ensuring product satisfaction and safety of consumers. This paper reports the qualitative and quantitative chemical analysis of the leaf ethanolic extract of C. asiatica using LCMS/MS. The acute toxiciy effect of the extract and selected marker chemical constituents were further analysed using a zebrafish model. Twenty constituents, were identified and the main chemical marker constituents of the plant viz asiaticoside, asiatic acid, and madecassic acid were further quantified. Asiaticoside was found to be present in higher concentration than the other marker constituents. Meanwhile in the acute toxicity test, the LD50 of the extract on the zebrafish model was determined to be 1250 mg/L while 100% mortality was observed at the highest test concentration of 2500 mg/L. However, acute toxicity evaluation on four marker triterpenoids of the herb, i.e asiatic acid, madecassic acid, asiaticoside and madecassoside, indicated them to be quite safe on the zebrafish model, with no mortality shown for test concentrations between 10 to 500 mg/kg BW.
format Article
author Zakaria, Fauziahanim
Wan Ibrahim, Wan Norhamidah
Ismail, Intan Safinar
Ahmad, Hafandi
Manshoor, Nurhuda
Ismail, Nor Hadiani
Zainal, Zurina
Shaari, Khozirah
spellingShingle Zakaria, Fauziahanim
Wan Ibrahim, Wan Norhamidah
Ismail, Intan Safinar
Ahmad, Hafandi
Manshoor, Nurhuda
Ismail, Nor Hadiani
Zainal, Zurina
Shaari, Khozirah
LCMS/MS metabolite profiling and analysis of acute toxicity effect of the ethanolic extract of Centella asiatica on zebrafish model
author_facet Zakaria, Fauziahanim
Wan Ibrahim, Wan Norhamidah
Ismail, Intan Safinar
Ahmad, Hafandi
Manshoor, Nurhuda
Ismail, Nor Hadiani
Zainal, Zurina
Shaari, Khozirah
author_sort Zakaria, Fauziahanim
title LCMS/MS metabolite profiling and analysis of acute toxicity effect of the ethanolic extract of Centella asiatica on zebrafish model
title_short LCMS/MS metabolite profiling and analysis of acute toxicity effect of the ethanolic extract of Centella asiatica on zebrafish model
title_full LCMS/MS metabolite profiling and analysis of acute toxicity effect of the ethanolic extract of Centella asiatica on zebrafish model
title_fullStr LCMS/MS metabolite profiling and analysis of acute toxicity effect of the ethanolic extract of Centella asiatica on zebrafish model
title_full_unstemmed LCMS/MS metabolite profiling and analysis of acute toxicity effect of the ethanolic extract of Centella asiatica on zebrafish model
title_sort lcms/ms metabolite profiling and analysis of acute toxicity effect of the ethanolic extract of centella asiatica on zebrafish model
publisher Universiti Putra Malaysia Press
publishDate 2019
url http://psasir.upm.edu.my/id/eprint/68709/1/28.%20JST%201366-2018.pdf
_version_ 1819298720737394688
score 13.4562235

Benzer Materyaller