Visualització del personal: Crude extracts of epidermal mucus and epidermis of climbing perch Anabas testudineus and its antibacterial and hemolytic activities

Crude extracts of epidermal mucus and epidermis of climbing perch Anabas testudineus and its antibacterial and hemolytic activities

Epidermal mucus of fish contains a number of biologically active constituents including antimicrobial peptides that are continuously expressed and also engaged in provision of protection to the fish against injurious substances as well as potential pathogenic microbes. This study seeks to determine...

Descripció completa

Guardat en:

Dades bibliogràfiques
Autors principals:	Al-Rasheed, Agharid, Handool, Kareem Obayes, Garba, Bashiru, Mohamed Mustapha, Noordin, Bejo, Siti Khairani, Mustaffa Kamal, Farina, Mohd Daud, Hassan
Format:	Article
Idioma:	English
Publicat:	Elsevier 2018
Accés en línia:	http://psasir.upm.edu.my/id/eprint/72144/1/Crude%20extracts%20of%20epidermal%20mucus%20and%20epidermis%20of%20climbing%20perch%20Anabas%20testudineus%20and%20its%20antibacterial%20and%20hemolytic%20activities.pdf
Etiquetes:	Afegir etiqueta Sense etiquetes, Sigues el primer a etiquetar aquest registre!

id	oai:psasir.upm.edu.my:72144
record_format	eprints
spelling	oai:psasir.upm.edu.my:72144 http://psasir.upm.edu.my/id/eprint/72144/ Crude extracts of epidermal mucus and epidermis of climbing perch Anabas testudineus and its antibacterial and hemolytic activities Al-Rasheed, Agharid Handool, Kareem Obayes Garba, Bashiru Mohamed Mustapha, Noordin Bejo, Siti Khairani Mustaffa Kamal, Farina Mohd Daud, Hassan Epidermal mucus of fish contains a number of biologically active constituents including antimicrobial peptides that are continuously expressed and also engaged in provision of protection to the fish against injurious substances as well as potential pathogenic microbes. This study seeks to determine the hemolytic and antibacterial properties of crude extract from epidermal mucus of climbing perch. Epidermal mucus and epidermis were obtained from 15 climbing perches after hypothermic stress stimulation for extraction of crude by aqueous and acidic methods. Antibacterial activity by well diffusion agar was applied for both extracts against 11 strains belonging to 7 different bacterial species using well agar diffusion test assay. In addition, haemolytic activity was investigated using horse RBCs. Antibacterial activity was performed for acidified crude extract only and it was found to be significant (P < 0.05) against P. aeruginosa while the least reactivity was observed against Methicillin Resistant Staphylococcus aureus. Total soluble protein concentration was 1.2 mg/ml with 13 bands of proteins (245–11) kDa distinguished. There was absence of haemolytic activity of acidified crude extract using horse RBCs. The finding of this study indicates that proteins in bioactive crude extracted from climbing perch have potential therapeutic application. Elsevier 2018-06 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/72144/1/Crude%20extracts%20of%20epidermal%20mucus%20and%20epidermis%20of%20climbing%20perch%20Anabas%20testudineus%20and%20its%20antibacterial%20and%20hemolytic%20activities.pdf Al-Rasheed, Agharid and Handool, Kareem Obayes and Garba, Bashiru and Mohamed Mustapha, Noordin and Bejo, Siti Khairani and Mustaffa Kamal, Farina and Mohd Daud, Hassan (2018) Crude extracts of epidermal mucus and epidermis of climbing perch Anabas testudineus and its antibacterial and hemolytic activities. Egyptian Journal of Aquatic Research, 44 (2). 125 - 129. ISSN 1687-4285; ESSN: 2090-3278 https://www.sciencedirect.com/science/article/pii/S1687428518300256 10.1016/j.ejar.2018.06.002
institution	UPM IR
collection	UPM IR
language	English
description	Epidermal mucus of fish contains a number of biologically active constituents including antimicrobial peptides that are continuously expressed and also engaged in provision of protection to the fish against injurious substances as well as potential pathogenic microbes. This study seeks to determine the hemolytic and antibacterial properties of crude extract from epidermal mucus of climbing perch. Epidermal mucus and epidermis were obtained from 15 climbing perches after hypothermic stress stimulation for extraction of crude by aqueous and acidic methods. Antibacterial activity by well diffusion agar was applied for both extracts against 11 strains belonging to 7 different bacterial species using well agar diffusion test assay. In addition, haemolytic activity was investigated using horse RBCs. Antibacterial activity was performed for acidified crude extract only and it was found to be significant (P < 0.05) against P. aeruginosa while the least reactivity was observed against Methicillin Resistant Staphylococcus aureus. Total soluble protein concentration was 1.2 mg/ml with 13 bands of proteins (245–11) kDa distinguished. There was absence of haemolytic activity of acidified crude extract using horse RBCs. The finding of this study indicates that proteins in bioactive crude extracted from climbing perch have potential therapeutic application.
format	Article
author	Al-Rasheed, Agharid Handool, Kareem Obayes Garba, Bashiru Mohamed Mustapha, Noordin Bejo, Siti Khairani Mustaffa Kamal, Farina Mohd Daud, Hassan
spellingShingle	Al-Rasheed, Agharid Handool, Kareem Obayes Garba, Bashiru Mohamed Mustapha, Noordin Bejo, Siti Khairani Mustaffa Kamal, Farina Mohd Daud, Hassan Crude extracts of epidermal mucus and epidermis of climbing perch Anabas testudineus and its antibacterial and hemolytic activities
author_facet	Al-Rasheed, Agharid Handool, Kareem Obayes Garba, Bashiru Mohamed Mustapha, Noordin Bejo, Siti Khairani Mustaffa Kamal, Farina Mohd Daud, Hassan
author_sort	Al-Rasheed, Agharid
title	Crude extracts of epidermal mucus and epidermis of climbing perch Anabas testudineus and its antibacterial and hemolytic activities
title_short	Crude extracts of epidermal mucus and epidermis of climbing perch Anabas testudineus and its antibacterial and hemolytic activities
title_full	Crude extracts of epidermal mucus and epidermis of climbing perch Anabas testudineus and its antibacterial and hemolytic activities
title_fullStr	Crude extracts of epidermal mucus and epidermis of climbing perch Anabas testudineus and its antibacterial and hemolytic activities
title_full_unstemmed	Crude extracts of epidermal mucus and epidermis of climbing perch Anabas testudineus and its antibacterial and hemolytic activities
title_sort	crude extracts of epidermal mucus and epidermis of climbing perch anabas testudineus and its antibacterial and hemolytic activities
publisher	Elsevier
publishDate	2018
url	http://psasir.upm.edu.my/id/eprint/72144/1/Crude%20extracts%20of%20epidermal%20mucus%20and%20epidermis%20of%20climbing%20perch%20Anabas%20testudineus%20and%20its%20antibacterial%20and%20hemolytic%20activities.pdf
_version_	1819299015048560640
score	13.4562235

Crude extracts of epidermal mucus and epidermis of climbing perch Anabas testudineus and its antibacterial and hemolytic activities

Ítems similars