প্রচ্ছদ

Digital massage for semen collection, evaluation and extension in Malaysian estuarine crocodile (Crocodylus porosus)

Study in semen of the estuarine crocodiles is limited partly due to the lack of knowledge on the semen collection method and their reproductive biology. Therefore, this study was conducted to determine the possibility of semen collection via digital manipulation of the penis. Four matured male croco...

সম্পূর্ণ বিবরণ

সংরক্ষণ করুন:
গ্রন্থ-পঞ্জীর বিবরন
প্রধান লেখক: Wan Jaafar, Wan Nor Fitri, Haron, Abd Wahid, Tengku Azizan, Tengku Rinalfi Putra, Yusoff, Rosnina, Raj, Dana, Yawah, Donny, Ab. Latip, Mohd Qayyum, Ab Aziz, Abdul Malek
বিন্যাস: প্রবন্ধ
ভাষা:English
প্রকাশিত: Elsevier 2018
অনলাইন ব্যবহার করুন:http://psasir.upm.edu.my/id/eprint/72303/1/Digital%20massage%20for%20semen%20collection.pdf
ট্যাগগুলো: ট্যাগ যুক্ত করুন
কোনো ট্যাগ নেই, প্রথমজন হিসাবে ট্যাগ করুন!
id oai:psasir.upm.edu.my:72303
record_format eprints
spelling oai:psasir.upm.edu.my:72303 http://psasir.upm.edu.my/id/eprint/72303/ Digital massage for semen collection, evaluation and extension in Malaysian estuarine crocodile (Crocodylus porosus) Wan Jaafar, Wan Nor Fitri Haron, Abd Wahid Tengku Azizan, Tengku Rinalfi Putra Yusoff, Rosnina Raj, Dana Yawah, Donny Ab. Latip, Mohd Qayyum Ab Aziz, Abdul Malek Study in semen of the estuarine crocodiles is limited partly due to the lack of knowledge on the semen collection method and their reproductive biology. Therefore, this study was conducted to determine the possibility of semen collection via digital manipulation of the penis. Four matured male crocodiles kept in the same enclosure were physically restrained for semen collection. The collection was done in January 2015 during crocodiles mating season. Mean (± SEM) seminal volume, mass movement, pH, sperm concentration, general motility, forward movement, rotating and vibrating movements measured were 0.55 ± 0.12 ml, 2.75 ± 0.48, 7.13 ± 0.24, 396.88 ± 43.41 × 106 sperm/ml, 45 ± 17.56%, 37.13 ± 5.12%, 9.56 ± 5.38% and 53.13 ± 1.92% respectively. The most important sperm abnormalities that requires further investigation is the cytoplasmic droplets which was 28.25 ± 2.29%. Sperm motility trend was best preserved in extender made up of 0.01 M PBS with 5% egg yolk. Sperm stored 4 °C in this extender had survived longer than the other two extender which was 11 days. There is a unique trend in the spike of spermatozoa motility post 48 h in chilled condition however the cause of such pattern is still unknown. Semen collection by digital manipulation of the penis was adequate in obtaining semen and the semen characteristics of the estuarine crocodile are described. Elsevier 2018-01 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/72303/1/Digital%20massage%20for%20semen%20collection.pdf Wan Jaafar, Wan Nor Fitri and Haron, Abd Wahid and Tengku Azizan, Tengku Rinalfi Putra and Yusoff, Rosnina and Raj, Dana and Yawah, Donny and Ab. Latip, Mohd Qayyum and Ab Aziz, Abdul Malek (2018) Digital massage for semen collection, evaluation and extension in Malaysian estuarine crocodile (Crocodylus porosus). Aquaculture, 483. 169 - 172. ISSN 0044-8486 https://www.sciencedirect.com/science/article/abs/pii/S0044848617315600 10.1016/j.aquaculture.2017.10.026
institution UPM IR
collection UPM IR
language English
description Study in semen of the estuarine crocodiles is limited partly due to the lack of knowledge on the semen collection method and their reproductive biology. Therefore, this study was conducted to determine the possibility of semen collection via digital manipulation of the penis. Four matured male crocodiles kept in the same enclosure were physically restrained for semen collection. The collection was done in January 2015 during crocodiles mating season. Mean (± SEM) seminal volume, mass movement, pH, sperm concentration, general motility, forward movement, rotating and vibrating movements measured were 0.55 ± 0.12 ml, 2.75 ± 0.48, 7.13 ± 0.24, 396.88 ± 43.41 × 106 sperm/ml, 45 ± 17.56%, 37.13 ± 5.12%, 9.56 ± 5.38% and 53.13 ± 1.92% respectively. The most important sperm abnormalities that requires further investigation is the cytoplasmic droplets which was 28.25 ± 2.29%. Sperm motility trend was best preserved in extender made up of 0.01 M PBS with 5% egg yolk. Sperm stored 4 °C in this extender had survived longer than the other two extender which was 11 days. There is a unique trend in the spike of spermatozoa motility post 48 h in chilled condition however the cause of such pattern is still unknown. Semen collection by digital manipulation of the penis was adequate in obtaining semen and the semen characteristics of the estuarine crocodile are described.
format Article
author Wan Jaafar, Wan Nor Fitri
Haron, Abd Wahid
Tengku Azizan, Tengku Rinalfi Putra
Yusoff, Rosnina
Raj, Dana
Yawah, Donny
Ab. Latip, Mohd Qayyum
Ab Aziz, Abdul Malek
spellingShingle Wan Jaafar, Wan Nor Fitri
Haron, Abd Wahid
Tengku Azizan, Tengku Rinalfi Putra
Yusoff, Rosnina
Raj, Dana
Yawah, Donny
Ab. Latip, Mohd Qayyum
Ab Aziz, Abdul Malek
Digital massage for semen collection, evaluation and extension in Malaysian estuarine crocodile (Crocodylus porosus)
author_facet Wan Jaafar, Wan Nor Fitri
Haron, Abd Wahid
Tengku Azizan, Tengku Rinalfi Putra
Yusoff, Rosnina
Raj, Dana
Yawah, Donny
Ab. Latip, Mohd Qayyum
Ab Aziz, Abdul Malek
author_sort Wan Jaafar, Wan Nor Fitri
title Digital massage for semen collection, evaluation and extension in Malaysian estuarine crocodile (Crocodylus porosus)
title_short Digital massage for semen collection, evaluation and extension in Malaysian estuarine crocodile (Crocodylus porosus)
title_full Digital massage for semen collection, evaluation and extension in Malaysian estuarine crocodile (Crocodylus porosus)
title_fullStr Digital massage for semen collection, evaluation and extension in Malaysian estuarine crocodile (Crocodylus porosus)
title_full_unstemmed Digital massage for semen collection, evaluation and extension in Malaysian estuarine crocodile (Crocodylus porosus)
title_sort digital massage for semen collection, evaluation and extension in malaysian estuarine crocodile (crocodylus porosus)
publisher Elsevier
publishDate 2018
url http://psasir.upm.edu.my/id/eprint/72303/1/Digital%20massage%20for%20semen%20collection.pdf
_version_ 1819299028421050368
score 13.4562235

অনুরূপ উপাদানগুলি