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Edible birds nest impact on rats uterine histomorphology, expressions of genes of growth factors and proliferating cell nuclear antigen, and oxidative stress level

Aim: This study aimed to evaluate the effect of edible bird’s nest (EBN) supplementation on the uteri of rats based on analyses of the morphological and histomorphometric changes, and expressions of epidermal growth factor (EGF) and its receptor (REGF) genes, vascular endothelial growth factor (VEGF...

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Main Authors: Albishtue, Abdulla A., Degu, Nurhusien Yimer, Abu Bakar @ Zakaria, Md Zuki, Haron, Abd Wahid, Yusoff, Rosnina, Assi, Mohammed A., Almhanawi, Bahaa H.
Format: Article
Language:English
Published: Veterinary World 2018
Online Access:http://psasir.upm.edu.my/id/eprint/72369/1/Edible%20bird%E2%80%99s%20nest%20impact%20on%20rats%E2%80%99%20uterine%20histomorphology.pdf
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spelling oai:psasir.upm.edu.my:72369 http://psasir.upm.edu.my/id/eprint/72369/ Edible birds nest impact on rats uterine histomorphology, expressions of genes of growth factors and proliferating cell nuclear antigen, and oxidative stress level Albishtue, Abdulla A. Degu, Nurhusien Yimer Abu Bakar @ Zakaria, Md Zuki Haron, Abd Wahid Yusoff, Rosnina Assi, Mohammed A. Almhanawi, Bahaa H. Aim: This study aimed to evaluate the effect of edible bird’s nest (EBN) supplementation on the uteri of rats based on analyses of the morphological and histomorphometric changes, and expressions of epidermal growth factor (EGF) and its receptor (REGF) genes, vascular endothelial growth factor (VEGF), proliferating cell nuclear antigen (PCNA), and steroid receptors. Materials and Methods: Twenty-four Sprague Dawley rats were equally distributed into the following four groups: G1 (control), G2, G3, and G4 represented the groups treated with EBN at graded concentrations of 0, 30, 60, and 120 mg/kg body weight (BW) per day for 8 weeks, respectively. During the experimental period, the BW of each rat was recorded weekly. At the proestrus stage of estrous cycle, blood samples were collected from the hearts of anesthetized rats that were later sacrificed. The uteri were removed for histological and immunohistochemical analyses. Results: The EBN-treated groups showed an increase in the weights and lengths of uteri as compared to the control. Results showed that relative to G1 and G2, G3 and G4 exhibited proliferation in their uterine luminal and glandular epithelia and uterine glands, and up-regulated expressions of EGF, REGF, VEGF, PCNA, and progesterone receptor, and estrogen receptor in their uteri. The EBN increased the antioxidant (AO) and total AO capacities and reduced the oxidative stress (OS) levels in non-pregnant rats. Conclusion: Findings of this study revealed that EBN promotes proliferation of the uterine structures as evidenced by the upregulation of the expressions of steroid receptors,EGF, REGF, VEGF, and PCNA in the uterus and increased in the plasma concentrations of AO and reduced levels of OS. Veterinary World 2018-01 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/72369/1/Edible%20bird%E2%80%99s%20nest%20impact%20on%20rats%E2%80%99%20uterine%20histomorphology.pdf Albishtue, Abdulla A. and Degu, Nurhusien Yimer and Abu Bakar @ Zakaria, Md Zuki and Haron, Abd Wahid and Yusoff, Rosnina and Assi, Mohammed A. and Almhanawi, Bahaa H. (2018) Edible birds nest impact on rats uterine histomorphology, expressions of genes of growth factors and proliferating cell nuclear antigen, and oxidative stress level. Veterinary World, 11 (1). 71 - 79. ISSN 0972-8988; ESSN: 2231-0916 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5813515/ 10.14202/vetworld.2018.71-79
institution UPM IR
collection UPM IR
language English
description Aim: This study aimed to evaluate the effect of edible bird’s nest (EBN) supplementation on the uteri of rats based on analyses of the morphological and histomorphometric changes, and expressions of epidermal growth factor (EGF) and its receptor (REGF) genes, vascular endothelial growth factor (VEGF), proliferating cell nuclear antigen (PCNA), and steroid receptors. Materials and Methods: Twenty-four Sprague Dawley rats were equally distributed into the following four groups: G1 (control), G2, G3, and G4 represented the groups treated with EBN at graded concentrations of 0, 30, 60, and 120 mg/kg body weight (BW) per day for 8 weeks, respectively. During the experimental period, the BW of each rat was recorded weekly. At the proestrus stage of estrous cycle, blood samples were collected from the hearts of anesthetized rats that were later sacrificed. The uteri were removed for histological and immunohistochemical analyses. Results: The EBN-treated groups showed an increase in the weights and lengths of uteri as compared to the control. Results showed that relative to G1 and G2, G3 and G4 exhibited proliferation in their uterine luminal and glandular epithelia and uterine glands, and up-regulated expressions of EGF, REGF, VEGF, PCNA, and progesterone receptor, and estrogen receptor in their uteri. The EBN increased the antioxidant (AO) and total AO capacities and reduced the oxidative stress (OS) levels in non-pregnant rats. Conclusion: Findings of this study revealed that EBN promotes proliferation of the uterine structures as evidenced by the upregulation of the expressions of steroid receptors,EGF, REGF, VEGF, and PCNA in the uterus and increased in the plasma concentrations of AO and reduced levels of OS.
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author Albishtue, Abdulla A.
Degu, Nurhusien Yimer
Abu Bakar @ Zakaria, Md Zuki
Haron, Abd Wahid
Yusoff, Rosnina
Assi, Mohammed A.
Almhanawi, Bahaa H.
spellingShingle Albishtue, Abdulla A.
Degu, Nurhusien Yimer
Abu Bakar @ Zakaria, Md Zuki
Haron, Abd Wahid
Yusoff, Rosnina
Assi, Mohammed A.
Almhanawi, Bahaa H.
Edible birds nest impact on rats uterine histomorphology, expressions of genes of growth factors and proliferating cell nuclear antigen, and oxidative stress level
author_facet Albishtue, Abdulla A.
Degu, Nurhusien Yimer
Abu Bakar @ Zakaria, Md Zuki
Haron, Abd Wahid
Yusoff, Rosnina
Assi, Mohammed A.
Almhanawi, Bahaa H.
author_sort Albishtue, Abdulla A.
title Edible birds nest impact on rats uterine histomorphology, expressions of genes of growth factors and proliferating cell nuclear antigen, and oxidative stress level
title_short Edible birds nest impact on rats uterine histomorphology, expressions of genes of growth factors and proliferating cell nuclear antigen, and oxidative stress level
title_full Edible birds nest impact on rats uterine histomorphology, expressions of genes of growth factors and proliferating cell nuclear antigen, and oxidative stress level
title_fullStr Edible birds nest impact on rats uterine histomorphology, expressions of genes of growth factors and proliferating cell nuclear antigen, and oxidative stress level
title_full_unstemmed Edible birds nest impact on rats uterine histomorphology, expressions of genes of growth factors and proliferating cell nuclear antigen, and oxidative stress level
title_sort edible birds nest impact on rats uterine histomorphology, expressions of genes of growth factors and proliferating cell nuclear antigen, and oxidative stress level
publisher Veterinary World
publishDate 2018
url http://psasir.upm.edu.my/id/eprint/72369/1/Edible%20bird%E2%80%99s%20nest%20impact%20on%20rats%E2%80%99%20uterine%20histomorphology.pdf
_version_ 1819299033836945408
score 13.4562235

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