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Autolysis of bovine skin, its endogenous proteases, protease inhibitors and their effects on quality characteristics of extracted gelatin

The autolysis of pretreated bovine skin (PBS) (treated with 0.1 M NaOH and 1% HCl), its endogenous proteases, inhibitors and their effects on quality attributes of gelatin were examined. PBS was subjected to different temperatures (20–90 °C) and pH (2–9) and treated with different protease inhibitor...

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প্রধান লেখক: Sazili, Awis Qurni, Dauda, Adeyemi Kazeem, Ahmad, Tanbir, Leo, Teik Kee, Mohamed, Elmutaz Atta Awad, Ahmad, Siti Aqlima, Ismail, Amin, Abdul Khalil, Khalilah
বিন্যাস: প্রবন্ধ
ভাষা:English
প্রকাশিত: Elsevier 2018
অনলাইন ব্যবহার করুন:http://psasir.upm.edu.my/id/eprint/73245/1/BOVINE.pdf
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id oai:psasir.upm.edu.my:73245
record_format eprints
spelling oai:psasir.upm.edu.my:73245 http://psasir.upm.edu.my/id/eprint/73245/ Autolysis of bovine skin, its endogenous proteases, protease inhibitors and their effects on quality characteristics of extracted gelatin Sazili, Awis Qurni Dauda, Adeyemi Kazeem Ahmad, Tanbir Leo, Teik Kee Mohamed, Elmutaz Atta Awad Ahmad, Siti Aqlima Ismail, Amin Abdul Khalil, Khalilah The autolysis of pretreated bovine skin (PBS) (treated with 0.1 M NaOH and 1% HCl), its endogenous proteases, inhibitors and their effects on quality attributes of gelatin were examined. PBS was subjected to different temperatures (20–90 °C) and pH (2–9) and treated with different protease inhibitors. Maximum autolytic activity of PBS was observed at 40 °C and pH 5. Ethylene-bis (oxyethylenenitrilo) tetraacetic acid (EGTA) was the most effective in impeding the degradation of γ-, β- and α- chains of PBS protein indicating that metallocollagenases were the predominant endogenous proteases in bovine skin. Gelatin was extracted in the absence (GAE) and presence (GPE) of EGTA, and EGTA with papain enzyme (GPEP). GPEP had a higher yield and lower gel strength than GEA and GPE. Metallocollagenases partook in the degradation of gelatin thereby affecting its functional properties. Pretreating PBS with or without EGTA, and papain influenced the quality attributes of gelatin. Elsevier 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/73245/1/BOVINE.pdf Sazili, Awis Qurni and Dauda, Adeyemi Kazeem and Ahmad, Tanbir and Leo, Teik Kee and Mohamed, Elmutaz Atta Awad and Ahmad, Siti Aqlima and Ismail, Amin and Abdul Khalil, Khalilah (2018) Autolysis of bovine skin, its endogenous proteases, protease inhibitors and their effects on quality characteristics of extracted gelatin. Food Chemistry, 265. pp. 1-8. ISSN 1873-7072; ESSN: 0308-8146 https://www.sciencedirect.com/science/article/abs/pii/S0308814618308410#! 10.1016/j.foodchem.2018.05.046
institution UPM IR
collection UPM IR
language English
description The autolysis of pretreated bovine skin (PBS) (treated with 0.1 M NaOH and 1% HCl), its endogenous proteases, inhibitors and their effects on quality attributes of gelatin were examined. PBS was subjected to different temperatures (20–90 °C) and pH (2–9) and treated with different protease inhibitors. Maximum autolytic activity of PBS was observed at 40 °C and pH 5. Ethylene-bis (oxyethylenenitrilo) tetraacetic acid (EGTA) was the most effective in impeding the degradation of γ-, β- and α- chains of PBS protein indicating that metallocollagenases were the predominant endogenous proteases in bovine skin. Gelatin was extracted in the absence (GAE) and presence (GPE) of EGTA, and EGTA with papain enzyme (GPEP). GPEP had a higher yield and lower gel strength than GEA and GPE. Metallocollagenases partook in the degradation of gelatin thereby affecting its functional properties. Pretreating PBS with or without EGTA, and papain influenced the quality attributes of gelatin.
format Article
author Sazili, Awis Qurni
Dauda, Adeyemi Kazeem
Ahmad, Tanbir
Leo, Teik Kee
Mohamed, Elmutaz Atta Awad
Ahmad, Siti Aqlima
Ismail, Amin
Abdul Khalil, Khalilah
spellingShingle Sazili, Awis Qurni
Dauda, Adeyemi Kazeem
Ahmad, Tanbir
Leo, Teik Kee
Mohamed, Elmutaz Atta Awad
Ahmad, Siti Aqlima
Ismail, Amin
Abdul Khalil, Khalilah
Autolysis of bovine skin, its endogenous proteases, protease inhibitors and their effects on quality characteristics of extracted gelatin
author_facet Sazili, Awis Qurni
Dauda, Adeyemi Kazeem
Ahmad, Tanbir
Leo, Teik Kee
Mohamed, Elmutaz Atta Awad
Ahmad, Siti Aqlima
Ismail, Amin
Abdul Khalil, Khalilah
author_sort Sazili, Awis Qurni
title Autolysis of bovine skin, its endogenous proteases, protease inhibitors and their effects on quality characteristics of extracted gelatin
title_short Autolysis of bovine skin, its endogenous proteases, protease inhibitors and their effects on quality characteristics of extracted gelatin
title_full Autolysis of bovine skin, its endogenous proteases, protease inhibitors and their effects on quality characteristics of extracted gelatin
title_fullStr Autolysis of bovine skin, its endogenous proteases, protease inhibitors and their effects on quality characteristics of extracted gelatin
title_full_unstemmed Autolysis of bovine skin, its endogenous proteases, protease inhibitors and their effects on quality characteristics of extracted gelatin
title_sort autolysis of bovine skin, its endogenous proteases, protease inhibitors and their effects on quality characteristics of extracted gelatin
publisher Elsevier
publishDate 2018
url http://psasir.upm.edu.my/id/eprint/73245/1/BOVINE.pdf
_version_ 1819299086587658240
score 13.4562235

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