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Sensitive leptospira DNA detection using tapered optical fiber sensor

This paper presents the development of tapered optical fiber sensor to detect a specific Leptospira bacteria DNA. The bacteria causes Leptospirosis, a deadly disease but with common early flu-like symptoms. Optical single mode fiber (SMF) of 125 μm diameter is tapered to produce 12 μm waist diameter...

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Main Authors: Zainuddin, Nurul Hida, Chee, Hui Yee, Ahmad, Muhammad Zamharir, Mahdi, Mohd Adzir, Abu Bakar, Muhammad Hafiz, Yaacob, Mohd Hanif
Format: Article
Language:English
Published: Wiley-VCH Verlag 2018
Online Access:http://psasir.upm.edu.my/id/eprint/73862/1/Sensitive%20leptospira%20DNA%20detection%20using%20tapered%20optical%20fiber%20sensor.pdf
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spelling oai:psasir.upm.edu.my:73862 http://psasir.upm.edu.my/id/eprint/73862/ Sensitive leptospira DNA detection using tapered optical fiber sensor Zainuddin, Nurul Hida Chee, Hui Yee Ahmad, Muhammad Zamharir Mahdi, Mohd Adzir Abu Bakar, Muhammad Hafiz Yaacob, Mohd Hanif This paper presents the development of tapered optical fiber sensor to detect a specific Leptospira bacteria DNA. The bacteria causes Leptospirosis, a deadly disease but with common early flu-like symptoms. Optical single mode fiber (SMF) of 125 μm diameter is tapered to produce 12 μm waist diameter and 15 cm length. The novel DNA-based optical fiber sensor is functionalized by incubating the tapered region with sodium hydroxide (NaOH), (3-Aminopropyl) triethoxysilane and glutaraldehyde. Probe DNA is immobilized onto the tapered region and subsequently hybridized by its complementary DNA (cDNA). The transmission spectra of the DNA-based optical fiber sensor are measured in the 1500 to 1600 nm wavelength range. It is discovered that the shift of the wavelength in the SMF sensor is linearly proportional with the increase in the cDNA concentrations from 0.1 to 1.0 nM. The sensitivity of the sensor toward DNA is measured to be 1.2862 nm/nM and able to detect as low as 0.1 fM. The sensor indicates high specificity when only minimal shift is detected for non-cDNA testing. The developed sensor is able to distinguish between actual DNA of Leptospira serovars (Canicola and Copenhageni) against Clostridium difficile (control sample) at very low (femtomolar) target concentrations. Wiley-VCH Verlag 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/73862/1/Sensitive%20leptospira%20DNA%20detection%20using%20tapered%20optical%20fiber%20sensor.pdf Zainuddin, Nurul Hida and Chee, Hui Yee and Ahmad, Muhammad Zamharir and Mahdi, Mohd Adzir and Abu Bakar, Muhammad Hafiz and Yaacob, Mohd Hanif (2018) Sensitive leptospira DNA detection using tapered optical fiber sensor. Journal of Biophotonics, 11 (8). art. no. 201700363. pp. 1-12. ISSN 1864-063X; ESSN: 1864-0648 https://onlinelibrary.wiley.com/doi/abs/10.1002/jbio.201700363 10.1002/jbio.201700363
institution UPM IR
collection UPM IR
language English
description This paper presents the development of tapered optical fiber sensor to detect a specific Leptospira bacteria DNA. The bacteria causes Leptospirosis, a deadly disease but with common early flu-like symptoms. Optical single mode fiber (SMF) of 125 μm diameter is tapered to produce 12 μm waist diameter and 15 cm length. The novel DNA-based optical fiber sensor is functionalized by incubating the tapered region with sodium hydroxide (NaOH), (3-Aminopropyl) triethoxysilane and glutaraldehyde. Probe DNA is immobilized onto the tapered region and subsequently hybridized by its complementary DNA (cDNA). The transmission spectra of the DNA-based optical fiber sensor are measured in the 1500 to 1600 nm wavelength range. It is discovered that the shift of the wavelength in the SMF sensor is linearly proportional with the increase in the cDNA concentrations from 0.1 to 1.0 nM. The sensitivity of the sensor toward DNA is measured to be 1.2862 nm/nM and able to detect as low as 0.1 fM. The sensor indicates high specificity when only minimal shift is detected for non-cDNA testing. The developed sensor is able to distinguish between actual DNA of Leptospira serovars (Canicola and Copenhageni) against Clostridium difficile (control sample) at very low (femtomolar) target concentrations.
format Article
author Zainuddin, Nurul Hida
Chee, Hui Yee
Ahmad, Muhammad Zamharir
Mahdi, Mohd Adzir
Abu Bakar, Muhammad Hafiz
Yaacob, Mohd Hanif
spellingShingle Zainuddin, Nurul Hida
Chee, Hui Yee
Ahmad, Muhammad Zamharir
Mahdi, Mohd Adzir
Abu Bakar, Muhammad Hafiz
Yaacob, Mohd Hanif
Sensitive leptospira DNA detection using tapered optical fiber sensor
author_facet Zainuddin, Nurul Hida
Chee, Hui Yee
Ahmad, Muhammad Zamharir
Mahdi, Mohd Adzir
Abu Bakar, Muhammad Hafiz
Yaacob, Mohd Hanif
author_sort Zainuddin, Nurul Hida
title Sensitive leptospira DNA detection using tapered optical fiber sensor
title_short Sensitive leptospira DNA detection using tapered optical fiber sensor
title_full Sensitive leptospira DNA detection using tapered optical fiber sensor
title_fullStr Sensitive leptospira DNA detection using tapered optical fiber sensor
title_full_unstemmed Sensitive leptospira DNA detection using tapered optical fiber sensor
title_sort sensitive leptospira dna detection using tapered optical fiber sensor
publisher Wiley-VCH Verlag
publishDate 2018
url http://psasir.upm.edu.my/id/eprint/73862/1/Sensitive%20leptospira%20DNA%20detection%20using%20tapered%20optical%20fiber%20sensor.pdf
_version_ 1819299132388409344
score 13.4562235

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