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Triple knockout of frdC gltA and pta genes enhanced PHA production in Escherichia coli

Polyhydroxyalkanoate (PHA) is a linear polyester produced through the fermentation of sugar or lipid. Biosynthesis of PHA comprises three enzymes known as acetyl-CoA acetyltransferase (phaA), acetoacetyl-CoA reductase (phaB) and PHA synthase (phaC). Comamonas sp. is one of the strains commonly used...

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Hoofdauteurs: Mohamed Biran, Nurhajirah, Mohd Yusoff, Mohd Zulkhairi, Maeda, Toshinari, Zakaria, Mohd Rafein, Yee, Lian Ngit, Hassana, Mohd Ali
Formaat: Artikel
Taal:English
Gepubliceerd in: Malaysian Society for Molecular Biology and Biotechnology 2018
Online toegang:http://psasir.upm.edu.my/id/eprint/74347/1/Triple%20knockout%20of%20frdC%20gltA%20and%20pta%20genes%20enhanced%20PHA%20production%20in%20Escherichia%20coli.pdf
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spelling oai:psasir.upm.edu.my:74347 http://psasir.upm.edu.my/id/eprint/74347/ Triple knockout of frdC gltA and pta genes enhanced PHA production in Escherichia coli Mohamed Biran, Nurhajirah Mohd Yusoff, Mohd Zulkhairi Maeda, Toshinari Zakaria, Mohd Rafein Yee, Lian Ngit Hassana, Mohd Ali Polyhydroxyalkanoate (PHA) is a linear polyester produced through the fermentation of sugar or lipid. Biosynthesis of PHA comprises three enzymes known as acetyl-CoA acetyltransferase (phaA), acetoacetyl-CoA reductase (phaB) and PHA synthase (phaC). Comamonas sp. is one of the strains commonly used for PHA production. In order to develop higher PHA production from bacterial respond strategy, PHA biosynthesis operon of Comamonas sp. EB172 was introduced into Escherichia coli BW25113 through a pGEM-T vector. E. coli was chosen due to the complete genome information available and the absence of depolymerisation gene, phaZ. In this study, the deletion of several single genes, which are frdC, gltA, and pta, was found to be associated with PHA metabolism activity in E. coli BW25113. P1 transduction was performed to construct multiple genes knockout. The engineered strain, E. coli BW25113 frdCgltApta::kan/pGEM’-phaCABCo, yielded the highest PHA production at 64 wt.% with 1.4 fold higher than that of control strain of E. coli BW25113/pGEM’-phaCABCo. This strain is potential for industrial application for higher PHA production from E. coli. Malaysian Society for Molecular Biology and Biotechnology 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/74347/1/Triple%20knockout%20of%20frdC%20gltA%20and%20pta%20genes%20enhanced%20PHA%20production%20in%20Escherichia%20coli.pdf Mohamed Biran, Nurhajirah and Mohd Yusoff, Mohd Zulkhairi and Maeda, Toshinari and Zakaria, Mohd Rafein and Yee, Lian Ngit and Hassana, Mohd Ali (2018) Triple knockout of frdC gltA and pta genes enhanced PHA production in Escherichia coli. Asia-Pacific Journal of Molecular Biology and Biotechnology, 26 (1). pp. 11-18. ISSN 0128-7451; EISSN: 2672-7277 http://www.msmbb.my/index.php/publication 10.35118/apjmbb.2018.026.1.02
institution UPM IR
collection UPM IR
language English
description Polyhydroxyalkanoate (PHA) is a linear polyester produced through the fermentation of sugar or lipid. Biosynthesis of PHA comprises three enzymes known as acetyl-CoA acetyltransferase (phaA), acetoacetyl-CoA reductase (phaB) and PHA synthase (phaC). Comamonas sp. is one of the strains commonly used for PHA production. In order to develop higher PHA production from bacterial respond strategy, PHA biosynthesis operon of Comamonas sp. EB172 was introduced into Escherichia coli BW25113 through a pGEM-T vector. E. coli was chosen due to the complete genome information available and the absence of depolymerisation gene, phaZ. In this study, the deletion of several single genes, which are frdC, gltA, and pta, was found to be associated with PHA metabolism activity in E. coli BW25113. P1 transduction was performed to construct multiple genes knockout. The engineered strain, E. coli BW25113 frdCgltApta::kan/pGEM’-phaCABCo, yielded the highest PHA production at 64 wt.% with 1.4 fold higher than that of control strain of E. coli BW25113/pGEM’-phaCABCo. This strain is potential for industrial application for higher PHA production from E. coli.
format Article
author Mohamed Biran, Nurhajirah
Mohd Yusoff, Mohd Zulkhairi
Maeda, Toshinari
Zakaria, Mohd Rafein
Yee, Lian Ngit
Hassana, Mohd Ali
spellingShingle Mohamed Biran, Nurhajirah
Mohd Yusoff, Mohd Zulkhairi
Maeda, Toshinari
Zakaria, Mohd Rafein
Yee, Lian Ngit
Hassana, Mohd Ali
Triple knockout of frdC gltA and pta genes enhanced PHA production in Escherichia coli
author_facet Mohamed Biran, Nurhajirah
Mohd Yusoff, Mohd Zulkhairi
Maeda, Toshinari
Zakaria, Mohd Rafein
Yee, Lian Ngit
Hassana, Mohd Ali
author_sort Mohamed Biran, Nurhajirah
title Triple knockout of frdC gltA and pta genes enhanced PHA production in Escherichia coli
title_short Triple knockout of frdC gltA and pta genes enhanced PHA production in Escherichia coli
title_full Triple knockout of frdC gltA and pta genes enhanced PHA production in Escherichia coli
title_fullStr Triple knockout of frdC gltA and pta genes enhanced PHA production in Escherichia coli
title_full_unstemmed Triple knockout of frdC gltA and pta genes enhanced PHA production in Escherichia coli
title_sort triple knockout of frdc glta and pta genes enhanced pha production in escherichia coli
publisher Malaysian Society for Molecular Biology and Biotechnology
publishDate 2018
url http://psasir.upm.edu.my/id/eprint/74347/1/Triple%20knockout%20of%20frdC%20gltA%20and%20pta%20genes%20enhanced%20PHA%20production%20in%20Escherichia%20coli.pdf
_version_ 1819299165448962048
score 13.4562235

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