In-house rose Bengal plate agglutination test (RBPT) for a rapid diagnosis of Brucellosis in goats in Malaysia
The Rose Bengal Plate Test (RBPT) antigens from Brucella melitensis local isolates (in-house RBPT) were prepared and compared with RBPT antigen for Brucellosis in sheep and goats prepared by Veterinary Laboratory Agency, UK. Eight hundred fifty-six sera samples, of which were collected from goats we...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Medwell Journals
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/7493/1/In-house%20rose%20Bengal%20plate%20agglutination%20test%20%28RBPT%29%20for%20a%20rapid%20diagnosis%20of%20Brucellosis%20in%20goats%20in%20Malaysia.pdf |
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| Summary: | The Rose Bengal Plate Test (RBPT) antigens from Brucella melitensis local isolates (in-house RBPT) were prepared and compared with RBPT antigen for Brucellosis in sheep and goats prepared by Veterinary Laboratory Agency, UK. Eight hundred fifty-six sera samples, of which were collected from goats were examined with the RBPT and Complement Fixation Test (CFT). The RBPT and CFT results showed that the in-house RBPT antigen was superior to the commercial prepared RBPT antigen (VLA, UK). Out of 856 sera analyzed by in-house RBPT, commercial RBPT and CFT, 30.84, 26.40 and 31.65% were found to be Brucella positive, respectively. The sensitivity calculated for the in-house RBPT compared with CFT was 85.24% whilst that of commercial RBPT was 78.59%. Therefore, it was conclude that in-house RBPT antigens could be prepared and used for epidemiological surveillance of Caprine brucellosis in Malaysia. |
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