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Mycobacterium avium subspecies paratuberculosis detection in beef cattle in Taman Pertanian Universiti, UPM

Mycobacterium avium subspecies paratuberculosis (MAP) is the aetiology agent causes Johne‟s disease which viewed as a chronic, contagious bacterial of intestinal tract in ruminant species. The infection that caused substantial losses to the farmer was characterized by chronic or intermittent d...

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Main Author: Ismail, Nur Farah Athirah
Format: Project Paper Report
Language:English
Published: 2016
Online Access:http://psasir.upm.edu.my/id/eprint/78172/1/FPV%202016%2011%20-%20IR.pdf
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spelling oai:psasir.upm.edu.my:78172 http://psasir.upm.edu.my/id/eprint/78172/ Mycobacterium avium subspecies paratuberculosis detection in beef cattle in Taman Pertanian Universiti, UPM Ismail, Nur Farah Athirah Mycobacterium avium subspecies paratuberculosis (MAP) is the aetiology agent causes Johne‟s disease which viewed as a chronic, contagious bacterial of intestinal tract in ruminant species. The infection that caused substantial losses to the farmer was characterized by chronic or intermittent diarrhoea, progressive weight loss, and decreased production. This study was conducted to determine the presence of MAP antigen and antibodies in the faeces and serum, respectively, in the cattle. A total of 213 faecal samples and 71 serum samples were collected from 71 cattle at Taman Pertanian Universiti (TPU), Universiti Putra Malaysia (UPM). These samples were tested using Ziehl-Neelsen acid-fast stain method for antigen detection and complement fixation test (CFT) for antibodies detection. Ziehl-Neelsen staining revealed that 60 (28.2%) of 213 samples were positive for M. avium antigen while, CFT revealed 3 (4.2%) of 71 serum samples were positive for the antibody. However, MAP is not the only acid fast organism; thus the determination of species of the organism should supported by faecal culture. In conclusion, this study showed that there is MAP infection in the cattle herd at TPU, UPM, with the presence of antigen causing agent and antibodies in faecal and serum samples, respectively, of cattle. 2016-03 Project Paper Report NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/78172/1/FPV%202016%2011%20-%20IR.pdf Ismail, Nur Farah Athirah (2016) Mycobacterium avium subspecies paratuberculosis detection in beef cattle in Taman Pertanian Universiti, UPM. [Project Paper Report]
institution UPM IR
collection UPM IR
language English
description Mycobacterium avium subspecies paratuberculosis (MAP) is the aetiology agent causes Johne‟s disease which viewed as a chronic, contagious bacterial of intestinal tract in ruminant species. The infection that caused substantial losses to the farmer was characterized by chronic or intermittent diarrhoea, progressive weight loss, and decreased production. This study was conducted to determine the presence of MAP antigen and antibodies in the faeces and serum, respectively, in the cattle. A total of 213 faecal samples and 71 serum samples were collected from 71 cattle at Taman Pertanian Universiti (TPU), Universiti Putra Malaysia (UPM). These samples were tested using Ziehl-Neelsen acid-fast stain method for antigen detection and complement fixation test (CFT) for antibodies detection. Ziehl-Neelsen staining revealed that 60 (28.2%) of 213 samples were positive for M. avium antigen while, CFT revealed 3 (4.2%) of 71 serum samples were positive for the antibody. However, MAP is not the only acid fast organism; thus the determination of species of the organism should supported by faecal culture. In conclusion, this study showed that there is MAP infection in the cattle herd at TPU, UPM, with the presence of antigen causing agent and antibodies in faecal and serum samples, respectively, of cattle.
format Project Paper Report
author Ismail, Nur Farah Athirah
spellingShingle Ismail, Nur Farah Athirah
Mycobacterium avium subspecies paratuberculosis detection in beef cattle in Taman Pertanian Universiti, UPM
author_facet Ismail, Nur Farah Athirah
author_sort Ismail, Nur Farah Athirah
title Mycobacterium avium subspecies paratuberculosis detection in beef cattle in Taman Pertanian Universiti, UPM
title_short Mycobacterium avium subspecies paratuberculosis detection in beef cattle in Taman Pertanian Universiti, UPM
title_full Mycobacterium avium subspecies paratuberculosis detection in beef cattle in Taman Pertanian Universiti, UPM
title_fullStr Mycobacterium avium subspecies paratuberculosis detection in beef cattle in Taman Pertanian Universiti, UPM
title_full_unstemmed Mycobacterium avium subspecies paratuberculosis detection in beef cattle in Taman Pertanian Universiti, UPM
title_sort mycobacterium avium subspecies paratuberculosis detection in beef cattle in taman pertanian universiti, upm
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/78172/1/FPV%202016%2011%20-%20IR.pdf
_version_ 1819299501080313856
score 13.4562235

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