Sequence analysis and expression of VP3 gene of chicken anaemia virus
Chicken anaemia virus (CAV) isolate BL-5 VP3 gene was amplified, sequenced and expressed in E. coli as a fusion protein together with a six-histidine tag. The deduced amino acid sequence of VP3 was identical to other CAV isolates Cux-1, A2 and CIA-1 except for a single amino acid substitution at pos...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Veterinary Association Malaysia
2003
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| Online Access: | http://psasir.upm.edu.my/id/eprint/7859/1/Sequence%20analysis%20and%20expression%20of%20VP3%20gene%20of%20chicken%20anemia%20virus.pdf |
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| Summary: | Chicken anaemia virus (CAV) isolate BL-5 VP3 gene was amplified, sequenced and expressed in E. coli as a fusion protein together with a six-histidine tag. The deduced amino acid sequence of VP3 was identical to other CAV isolates Cux-1, A2 and CIA-1 except for a single amino acid substitution at position 12. SDS-PAGE and Western blot analysis indicated that the expressed protein is insoluble and was found primarily from the cell lysate fraction. Expression of the protein was detected as early as 1 h, with maximum expression (~12% of the total protein) at 6 h post induction with IPTG. This study indicates that VP3 protein is highly expressed in insoluble form in E. coli. However, the biological function of the protein remains to be studied |
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