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Effects of four extenders on the quality of frozen semen in Arabian stallions

Aim: Different types of extenders have a variety of components which show the tolerance effect on sperm protection during freezing procedures. In the present study, we have examined the impact of the extenders HF-20 and Tris, which were locally manufactured, and they are competing with commercial ex...

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Huvudupphovsmän: Alamaary, Mohaammed Saad, Haron, Abd Wahid, Ali, Mohamed, Hiew, Mark Wen Han, Adamu, Lawan, Peter, Innocent Damudu
Materialtyp: Artikel
Språk:English
Publicerad: Veterinary World 2019
Länkar:http://psasir.upm.edu.my/id/eprint/79593/1/Effects%20of%20four%20extenders%20on%20the%20quality%20of%20frozen%20semen%20in%20Arabian%20stallions%20.pdf
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spelling oai:psasir.upm.edu.my:79593 http://psasir.upm.edu.my/id/eprint/79593/ Effects of four extenders on the quality of frozen semen in Arabian stallions Alamaary, Mohaammed Saad Haron, Abd Wahid Ali, Mohamed Hiew, Mark Wen Han Adamu, Lawan Peter, Innocent Damudu Aim: Different types of extenders have a variety of components which show the tolerance effect on sperm protection during freezing procedures. In the present study, we have examined the impact of the extenders HF-20 and Tris, which were locally manufactured, and they are competing with commercial extenders INRA Freeze® (IMV Technologies, France) and EquiPlus Freeze® (Minitube, Germany) on the quality of horses frozen semen. Materials and Methods: A total of 15 ejaculates from three healthy stallions were collected and cryopreserved in the same environment. Each semen sample collected was divided into four equal parts and processed. All samples were analyzed before and after freezing for motility, viability, plasma membrane integrity, and morphology. Furthermore, twenty mares were inseminated using post-thawed semen. Results: There were no differences observed among all extenders in all the parameters before freezing. Sperm cryopreserved using HF-20 showed better motility, viability, and plasma membrane integrity than Tris extender. The Tris extender showed the most inferior quality of post-thawed semen between all the extenders. HF-20, INRA Freeze®, and EquiPlus Freeze® extenders revealed the same capacity of semen preservation in vitro and in vivo. Conclusion: HF-20 extender has the same quality as INRA Freeze® and EquiPlus Freeze® that can be considered as one of the best extenders for the semen cryopreservation in horses. In contrast, Tris extender needs some degree of improvement. Veterinary World 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/79593/1/Effects%20of%20four%20extenders%20on%20the%20quality%20of%20frozen%20semen%20in%20Arabian%20stallions%20.pdf Alamaary, Mohaammed Saad and Haron, Abd Wahid and Ali, Mohamed and Hiew, Mark Wen Han and Adamu, Lawan and Peter, Innocent Damudu (2019) Effects of four extenders on the quality of frozen semen in Arabian stallions. Veterinary World, 12 (1). pp. 34-40. ISSN 0972-8988; ESSN: 2231-0916 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6431823/ 10.14202/vetworld.2019.34-40
institution UPM IR
collection UPM IR
language English
description Aim: Different types of extenders have a variety of components which show the tolerance effect on sperm protection during freezing procedures. In the present study, we have examined the impact of the extenders HF-20 and Tris, which were locally manufactured, and they are competing with commercial extenders INRA Freeze® (IMV Technologies, France) and EquiPlus Freeze® (Minitube, Germany) on the quality of horses frozen semen. Materials and Methods: A total of 15 ejaculates from three healthy stallions were collected and cryopreserved in the same environment. Each semen sample collected was divided into four equal parts and processed. All samples were analyzed before and after freezing for motility, viability, plasma membrane integrity, and morphology. Furthermore, twenty mares were inseminated using post-thawed semen. Results: There were no differences observed among all extenders in all the parameters before freezing. Sperm cryopreserved using HF-20 showed better motility, viability, and plasma membrane integrity than Tris extender. The Tris extender showed the most inferior quality of post-thawed semen between all the extenders. HF-20, INRA Freeze®, and EquiPlus Freeze® extenders revealed the same capacity of semen preservation in vitro and in vivo. Conclusion: HF-20 extender has the same quality as INRA Freeze® and EquiPlus Freeze® that can be considered as one of the best extenders for the semen cryopreservation in horses. In contrast, Tris extender needs some degree of improvement.
format Article
author Alamaary, Mohaammed Saad
Haron, Abd Wahid
Ali, Mohamed
Hiew, Mark Wen Han
Adamu, Lawan
Peter, Innocent Damudu
spellingShingle Alamaary, Mohaammed Saad
Haron, Abd Wahid
Ali, Mohamed
Hiew, Mark Wen Han
Adamu, Lawan
Peter, Innocent Damudu
Effects of four extenders on the quality of frozen semen in Arabian stallions
author_facet Alamaary, Mohaammed Saad
Haron, Abd Wahid
Ali, Mohamed
Hiew, Mark Wen Han
Adamu, Lawan
Peter, Innocent Damudu
author_sort Alamaary, Mohaammed Saad
title Effects of four extenders on the quality of frozen semen in Arabian stallions
title_short Effects of four extenders on the quality of frozen semen in Arabian stallions
title_full Effects of four extenders on the quality of frozen semen in Arabian stallions
title_fullStr Effects of four extenders on the quality of frozen semen in Arabian stallions
title_full_unstemmed Effects of four extenders on the quality of frozen semen in Arabian stallions
title_sort effects of four extenders on the quality of frozen semen in arabian stallions
publisher Veterinary World
publishDate 2019
url http://psasir.upm.edu.my/id/eprint/79593/1/Effects%20of%20four%20extenders%20on%20the%20quality%20of%20frozen%20semen%20in%20Arabian%20stallions%20.pdf
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score 13.4562235

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