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Effects of plant growth regulators and activated charcoal on somaclonal variations of protocorm-like bodies (PLBs) of Dendrobium Sabin Blue orchid

Protocorm-like bodies (PLBs) of Dendrobium orchids are emerging as a potential source of valuable secondary metabolites. This study examined the effect of four additives namely 1-naphthaleneacetic acid (NAA), kinetin, thidiazuron (TDZ), and activated charcoal (AC) used in culture medium on genetic v...

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Main Authors: Chin, Chee Keong, Lee, Ze Hong, Mubbarakh, Safiah Ahmad, James Antony, Jessica Jeyanthi, Chew, Bee Lynn, Subramaniam, Sreeramanan
Format: Article
Language:English
Published: Elsevier 2019
Online Access:http://psasir.upm.edu.my/id/eprint/79611/1/Effects%20of%20plant%20growth%20regulators%20and%20activated%20charcoal%20on%20somaclonal%20variations%20of%20protocorm-like%20bodies%20%28PLBs%29%20of%20Dendrobium%20Sabin%20Blue%20orchid.pdf
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spelling oai:psasir.upm.edu.my:79611 http://psasir.upm.edu.my/id/eprint/79611/ Effects of plant growth regulators and activated charcoal on somaclonal variations of protocorm-like bodies (PLBs) of Dendrobium Sabin Blue orchid Chin, Chee Keong Lee, Ze Hong Mubbarakh, Safiah Ahmad James Antony, Jessica Jeyanthi Chew, Bee Lynn Subramaniam, Sreeramanan Protocorm-like bodies (PLBs) of Dendrobium orchids are emerging as a potential source of valuable secondary metabolites. This study examined the effect of four additives namely 1-naphthaleneacetic acid (NAA), kinetin, thidiazuron (TDZ), and activated charcoal (AC) used in culture medium on genetic variability in PLBs of Dendrobium Sabin Blue. Nine (9) ISSR primers and eleven (11) DAMD primers were used to assess the genetic variability of PLBs that were subcultured over a period of two years. We confirmed that the use of kinetin in culture medium for two years resulted in the highest rate of somaclonal variation in PLBs. On the other hand, TDZ and activated charcoal registered the lowest genetic variability in PLBs. The findings of this study suggest the importance of selecting additives used in the culture medium to maintain stable genetic lines of PLBs. We recommend that the assessment of somaclonal variations should be performed for long term maintenance of tissue cultures. Elsevier 2019-11 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/79611/1/Effects%20of%20plant%20growth%20regulators%20and%20activated%20charcoal%20on%20somaclonal%20variations%20of%20protocorm-like%20bodies%20%28PLBs%29%20of%20Dendrobium%20Sabin%20Blue%20orchid.pdf Chin, Chee Keong and Lee, Ze Hong and Mubbarakh, Safiah Ahmad and James Antony, Jessica Jeyanthi and Chew, Bee Lynn and Subramaniam, Sreeramanan (2019) Effects of plant growth regulators and activated charcoal on somaclonal variations of protocorm-like bodies (PLBs) of Dendrobium Sabin Blue orchid. Biocatalysis and Agricultural Biotechnology, 22 (1). pp. 1-8. ISSN 1878-8181 https://www.sciencedirect.com/science/article/abs/pii/S1878818119310321 10.1016/j.bcab.2019.101426
institution UPM IR
collection UPM IR
language English
description Protocorm-like bodies (PLBs) of Dendrobium orchids are emerging as a potential source of valuable secondary metabolites. This study examined the effect of four additives namely 1-naphthaleneacetic acid (NAA), kinetin, thidiazuron (TDZ), and activated charcoal (AC) used in culture medium on genetic variability in PLBs of Dendrobium Sabin Blue. Nine (9) ISSR primers and eleven (11) DAMD primers were used to assess the genetic variability of PLBs that were subcultured over a period of two years. We confirmed that the use of kinetin in culture medium for two years resulted in the highest rate of somaclonal variation in PLBs. On the other hand, TDZ and activated charcoal registered the lowest genetic variability in PLBs. The findings of this study suggest the importance of selecting additives used in the culture medium to maintain stable genetic lines of PLBs. We recommend that the assessment of somaclonal variations should be performed for long term maintenance of tissue cultures.
format Article
author Chin, Chee Keong
Lee, Ze Hong
Mubbarakh, Safiah Ahmad
James Antony, Jessica Jeyanthi
Chew, Bee Lynn
Subramaniam, Sreeramanan
spellingShingle Chin, Chee Keong
Lee, Ze Hong
Mubbarakh, Safiah Ahmad
James Antony, Jessica Jeyanthi
Chew, Bee Lynn
Subramaniam, Sreeramanan
Effects of plant growth regulators and activated charcoal on somaclonal variations of protocorm-like bodies (PLBs) of Dendrobium Sabin Blue orchid
author_facet Chin, Chee Keong
Lee, Ze Hong
Mubbarakh, Safiah Ahmad
James Antony, Jessica Jeyanthi
Chew, Bee Lynn
Subramaniam, Sreeramanan
author_sort Chin, Chee Keong
title Effects of plant growth regulators and activated charcoal on somaclonal variations of protocorm-like bodies (PLBs) of Dendrobium Sabin Blue orchid
title_short Effects of plant growth regulators and activated charcoal on somaclonal variations of protocorm-like bodies (PLBs) of Dendrobium Sabin Blue orchid
title_full Effects of plant growth regulators and activated charcoal on somaclonal variations of protocorm-like bodies (PLBs) of Dendrobium Sabin Blue orchid
title_fullStr Effects of plant growth regulators and activated charcoal on somaclonal variations of protocorm-like bodies (PLBs) of Dendrobium Sabin Blue orchid
title_full_unstemmed Effects of plant growth regulators and activated charcoal on somaclonal variations of protocorm-like bodies (PLBs) of Dendrobium Sabin Blue orchid
title_sort effects of plant growth regulators and activated charcoal on somaclonal variations of protocorm-like bodies (plbs) of dendrobium sabin blue orchid
publisher Elsevier
publishDate 2019
url http://psasir.upm.edu.my/id/eprint/79611/1/Effects%20of%20plant%20growth%20regulators%20and%20activated%20charcoal%20on%20somaclonal%20variations%20of%20protocorm-like%20bodies%20%28PLBs%29%20of%20Dendrobium%20Sabin%20Blue%20orchid.pdf
_version_ 1782759180737708032
score 12.933938

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