Staff View: Histopathological and cholinesterase changes in the gills of Clarias gariepinus as a result of cadmium exposure

Histopathological and cholinesterase changes in the gills of Clarias gariepinus as a result of cadmium exposure

Aim : The cholinesterase (ChE) based inhibition and histopathological studies from fish were investigated and represented in this study to develop as one of the great potential biomarkers for heavy metals monitoring. Methodology : In this study, the histopathological study of gills were observed a u...

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Main Authors:	Pariza, A. A. F. M., Ahmad, Siti Aqlima, Fadzil, Nadia Izati, Basirun, Ain Aqilah, Abdul Wahab Sha'arani, Shakirah, Mohamad Asri, Nurul Aida, Sabullah, Mohd Khalizan, Khalid, Ariff, Abd. Shukor, Mohd Yunus
Format:	Article
Language:	English
Published:	Triveni Enterprises 2019
Online Access:	http://psasir.upm.edu.my/id/eprint/80068/1/Histopathological%20and%20cholinesterase%20changes%20in%20the%20gills%20of%20Clarias%20gariepinus%20as%20a%20result%20of%20cadmium%20exposure.pdf
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spelling	oai:psasir.upm.edu.my:80068 http://psasir.upm.edu.my/id/eprint/80068/ Histopathological and cholinesterase changes in the gills of Clarias gariepinus as a result of cadmium exposure Pariza, A. A. F. M. Ahmad, Siti Aqlima Fadzil, Nadia Izati Basirun, Ain Aqilah Abdul Wahab Sha'arani, Shakirah Mohamad Asri, Nurul Aida Sabullah, Mohd Khalizan Khalid, Ariff Abd. Shukor, Mohd Yunus Aim : The cholinesterase (ChE) based inhibition and histopathological studies from fish were investigated and represented in this study to develop as one of the great potential biomarkers for heavy metals monitoring. Methodology : In this study, the histopathological study of gills were observed a under microscope. The capability of ChE extracted from the gills of Clarias gariepinus was assessed for declining Cd. ChE was purified through affinity chromatography and continued with the optimisation and inhibition study (IC50) of cholinesterase. Results : Histopathological study of gills was carried out and several changes such as aneurysm, necrosis and lamella fusion were noted. Purification fold obtained from purified enzyme was 1.15 with 30% a yield specific activity 20.726. The optimum temperature for purified AChE was 35°C along with acetylthiocholine iodide (ATC) as a preferable substrate that had the highest Vmax value of 0.5452 U mg'1 and the lowest Km value of 0.0311 mM. The optimum pH was observed to be 10 of Tris-HCl as a medium. Meanwhile, the IC50 of cadmium was 6.808 mg ľ with R2 value of 0.9532. Interpretation : The result of the study can be used as a tool for further developing a biomarker for the detection of heavy metals in aquatic ecosystems. In addition, the baseline data provided can also be used for designing a kit, which would give rapid and accurate result. Triveni Enterprises 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/80068/1/Histopathological%20and%20cholinesterase%20changes%20in%20the%20gills%20of%20Clarias%20gariepinus%20as%20a%20result%20of%20cadmium%20exposure.pdf Pariza, A. A. F. M. and Ahmad, Siti Aqlima and Fadzil, Nadia Izati and Basirun, Ain Aqilah and Abdul Wahab Sha'arani, Shakirah and Mohamad Asri, Nurul Aida and Sabullah, Mohd Khalizan and Khalid, Ariff and Abd. Shukor, Mohd Yunus (2019) Histopathological and cholinesterase changes in the gills of Clarias gariepinus as a result of cadmium exposure. Journal of Environmental Biology, 40 (4). pp. 683-690. ISSN 0254-8704; ESSN: 2394-0379 https://search.proquest.com/docview/2268536878?pq-origsite=gscholar&fromopenview=true 10.22438/jeb/40/4/MRN-579
institution	UPM IR
collection	UPM IR
language	English
description	Aim : The cholinesterase (ChE) based inhibition and histopathological studies from fish were investigated and represented in this study to develop as one of the great potential biomarkers for heavy metals monitoring. Methodology : In this study, the histopathological study of gills were observed a under microscope. The capability of ChE extracted from the gills of Clarias gariepinus was assessed for declining Cd. ChE was purified through affinity chromatography and continued with the optimisation and inhibition study (IC50) of cholinesterase. Results : Histopathological study of gills was carried out and several changes such as aneurysm, necrosis and lamella fusion were noted. Purification fold obtained from purified enzyme was 1.15 with 30% a yield specific activity 20.726. The optimum temperature for purified AChE was 35°C along with acetylthiocholine iodide (ATC) as a preferable substrate that had the highest Vmax value of 0.5452 U mg'1 and the lowest Km value of 0.0311 mM. The optimum pH was observed to be 10 of Tris-HCl as a medium. Meanwhile, the IC50 of cadmium was 6.808 mg ľ with R2 value of 0.9532. Interpretation : The result of the study can be used as a tool for further developing a biomarker for the detection of heavy metals in aquatic ecosystems. In addition, the baseline data provided can also be used for designing a kit, which would give rapid and accurate result.
format	Article
author	Pariza, A. A. F. M. Ahmad, Siti Aqlima Fadzil, Nadia Izati Basirun, Ain Aqilah Abdul Wahab Sha'arani, Shakirah Mohamad Asri, Nurul Aida Sabullah, Mohd Khalizan Khalid, Ariff Abd. Shukor, Mohd Yunus
spellingShingle	Pariza, A. A. F. M. Ahmad, Siti Aqlima Fadzil, Nadia Izati Basirun, Ain Aqilah Abdul Wahab Sha'arani, Shakirah Mohamad Asri, Nurul Aida Sabullah, Mohd Khalizan Khalid, Ariff Abd. Shukor, Mohd Yunus Histopathological and cholinesterase changes in the gills of Clarias gariepinus as a result of cadmium exposure
author_facet	Pariza, A. A. F. M. Ahmad, Siti Aqlima Fadzil, Nadia Izati Basirun, Ain Aqilah Abdul Wahab Sha'arani, Shakirah Mohamad Asri, Nurul Aida Sabullah, Mohd Khalizan Khalid, Ariff Abd. Shukor, Mohd Yunus
author_sort	Pariza, A. A. F. M.
title	Histopathological and cholinesterase changes in the gills of Clarias gariepinus as a result of cadmium exposure
title_short	Histopathological and cholinesterase changes in the gills of Clarias gariepinus as a result of cadmium exposure
title_full	Histopathological and cholinesterase changes in the gills of Clarias gariepinus as a result of cadmium exposure
title_fullStr	Histopathological and cholinesterase changes in the gills of Clarias gariepinus as a result of cadmium exposure
title_full_unstemmed	Histopathological and cholinesterase changes in the gills of Clarias gariepinus as a result of cadmium exposure
title_sort	histopathological and cholinesterase changes in the gills of clarias gariepinus as a result of cadmium exposure
publisher	Triveni Enterprises
publishDate	2019
url	http://psasir.upm.edu.my/id/eprint/80068/1/Histopathological%20and%20cholinesterase%20changes%20in%20the%20gills%20of%20Clarias%20gariepinus%20as%20a%20result%20of%20cadmium%20exposure.pdf
_version_	1819299643277705216
score	13.4562235

Histopathological and cholinesterase changes in the gills of Clarias gariepinus as a result of cadmium exposure

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