Improvement of extracellular secretion efficiency of recombinant protein from Escherichia coli: signal peptide fusion, surfactants addition, and phospholipase
The secretion of heterologous proteins into Escherichia coli cell culture media offers significant advantages for downstream processing, which can avoid the production of inclusion bodies, cost and time savings, and endotoxin reduction. However, E. coli does not secrete proteins into the extracellul...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Association of Pharmaceutical Innovators
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/80194/1/Improvement%20of%20extracellular%20secretion%20efficiency%20of%20recombinant%20protein%20from%20Escherichia%20coli%20signal%20peptide%20fusion%2C%20surfactants%20addition%2C%20and%20phospholipase.pdf |
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