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Antioxidant potential and Angiotensin-Converting Enzyme (ACE) inhibitory activity of orotic acid-loaded gum arabic nanoparticles

Orotic acid (OA) nanoparticles were prepared using the freeze-drying method. The antihypertensive activity and antioxidant capacity of OA and orotic acid-loaded gum arabic nanoparticles (OAGANPs) were examined using the angiotensin-converting enzyme (ACE), 1,1-diphenyl-2-picrylhydrazyl (DPPH), nitri...

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Main Authors: Abdelkader, Hassani, Hussain, Siti Aslina, Abdullah, Norhafizah, Kamarudin, Suryani, Rosli, Rozita
Format: Article
Language:English
Published: Springer 2019
Online Access:http://psasir.upm.edu.my/id/eprint/80682/1/ACE.pdf
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spelling oai:psasir.upm.edu.my:80682 http://psasir.upm.edu.my/id/eprint/80682/ Antioxidant potential and Angiotensin-Converting Enzyme (ACE) inhibitory activity of orotic acid-loaded gum arabic nanoparticles Abdelkader, Hassani Hussain, Siti Aslina Abdullah, Norhafizah Kamarudin, Suryani Rosli, Rozita Orotic acid (OA) nanoparticles were prepared using the freeze-drying method. The antihypertensive activity and antioxidant capacity of OA and orotic acid-loaded gum arabic nanoparticles (OAGANPs) were examined using the angiotensin-converting enzyme (ACE), 1,1-diphenyl-2-picrylhydrazyl (DPPH), nitric oxide (NO), and β-carotene assays, as well as the quantification of total phenolic content (TPC). The DPPH and NO scavenging activities of OAGANPs were significantly higher than those of the OA solution. The β-carotene bleaching assay of OAGANPs showed a dose-dependent trend, while 500 μg/ml was significantly more effective than the other concentrations, which exerted 63.4% of the antioxidant activity. The in vitro antihypertensive assay revealed that the OAGANPs exhibited the most potent ACE inhibition activity, when compared to the OA solution. Hence, results revealed the potential of preparing the OA as a nanoparticle formulation in enhancing the antioxidant and antihypertensive properties compared to the OA solution. Springer 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/80682/1/ACE.pdf Abdelkader, Hassani and Hussain, Siti Aslina and Abdullah, Norhafizah and Kamarudin, Suryani and Rosli, Rozita (2019) Antioxidant potential and Angiotensin-Converting Enzyme (ACE) inhibitory activity of orotic acid-loaded gum arabic nanoparticles. AAPS PharmSciTech, 20 (2). art. no. 53. pp. 1-9. ISSN 1530-9932 https://pubmed.ncbi.nlm.nih.gov/30617521/ 10.1208/s12249-018-1238-2
institution UPM IR
collection UPM IR
language English
description Orotic acid (OA) nanoparticles were prepared using the freeze-drying method. The antihypertensive activity and antioxidant capacity of OA and orotic acid-loaded gum arabic nanoparticles (OAGANPs) were examined using the angiotensin-converting enzyme (ACE), 1,1-diphenyl-2-picrylhydrazyl (DPPH), nitric oxide (NO), and β-carotene assays, as well as the quantification of total phenolic content (TPC). The DPPH and NO scavenging activities of OAGANPs were significantly higher than those of the OA solution. The β-carotene bleaching assay of OAGANPs showed a dose-dependent trend, while 500 μg/ml was significantly more effective than the other concentrations, which exerted 63.4% of the antioxidant activity. The in vitro antihypertensive assay revealed that the OAGANPs exhibited the most potent ACE inhibition activity, when compared to the OA solution. Hence, results revealed the potential of preparing the OA as a nanoparticle formulation in enhancing the antioxidant and antihypertensive properties compared to the OA solution.
format Article
author Abdelkader, Hassani
Hussain, Siti Aslina
Abdullah, Norhafizah
Kamarudin, Suryani
Rosli, Rozita
spellingShingle Abdelkader, Hassani
Hussain, Siti Aslina
Abdullah, Norhafizah
Kamarudin, Suryani
Rosli, Rozita
Antioxidant potential and Angiotensin-Converting Enzyme (ACE) inhibitory activity of orotic acid-loaded gum arabic nanoparticles
author_facet Abdelkader, Hassani
Hussain, Siti Aslina
Abdullah, Norhafizah
Kamarudin, Suryani
Rosli, Rozita
author_sort Abdelkader, Hassani
title Antioxidant potential and Angiotensin-Converting Enzyme (ACE) inhibitory activity of orotic acid-loaded gum arabic nanoparticles
title_short Antioxidant potential and Angiotensin-Converting Enzyme (ACE) inhibitory activity of orotic acid-loaded gum arabic nanoparticles
title_full Antioxidant potential and Angiotensin-Converting Enzyme (ACE) inhibitory activity of orotic acid-loaded gum arabic nanoparticles
title_fullStr Antioxidant potential and Angiotensin-Converting Enzyme (ACE) inhibitory activity of orotic acid-loaded gum arabic nanoparticles
title_full_unstemmed Antioxidant potential and Angiotensin-Converting Enzyme (ACE) inhibitory activity of orotic acid-loaded gum arabic nanoparticles
title_sort antioxidant potential and angiotensin-converting enzyme (ace) inhibitory activity of orotic acid-loaded gum arabic nanoparticles
publisher Springer
publishDate 2019
url http://psasir.upm.edu.my/id/eprint/80682/1/ACE.pdf
_version_ 1819299682898149376
score 13.4562235

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