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Assessment of heavy metal toxicity using a luminescent bacterial test based on Photobacterium sp. strain MIE

Toxicity evaluation of wastewater, polluted sediment and water streams is a very crucial aspect of environmental pollution monitoring. In this work, a newly developed luminescent bacterial test using a tropical luminescent bacterium, Photobacterium sp. strain MIE was used to assess the toxicity of s...

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Hoofdauteurs: Halmi, Mohd Izuan Effendi, Kassim, Adibah, Abd. Shukor, Mohd. Yunus
Formaat: Artikel
Taal:English
Gepubliceerd in: Springer 2019
Online toegang:http://psasir.upm.edu.my/id/eprint/80742/1/TOXIC.pdf
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spelling oai:psasir.upm.edu.my:80742 http://psasir.upm.edu.my/id/eprint/80742/ Assessment of heavy metal toxicity using a luminescent bacterial test based on Photobacterium sp. strain MIE Halmi, Mohd Izuan Effendi Kassim, Adibah Abd. Shukor, Mohd. Yunus Toxicity evaluation of wastewater, polluted sediment and water streams is a very crucial aspect of environmental pollution monitoring. In this work, a newly developed luminescent bacterial test using a tropical luminescent bacterium, Photobacterium sp. strain MIE was used to assess the toxicity of several heavy metals using a 15-min assay format. The assessment was carried out by exposing strain MIE to different concentrations of heavy metals ranging from 0.001 to 200 mg/L in a DTX microplate 96 wells. The toxicity result based on the inhibitory concentration (IC50) was Hg (0.053 mg/L) > Ag (0.12 mg/L) > Cu (0.85 mg/L) > Ni (12.32 mg/L) > Zn (18.72 mg/L) > Cr (26.02 mg/L). Principal Component Analysis (PCA) and Agglomerative Hierarchical Clustering (AHC) analyses showed the sensitivity (IC50) of strain MIE to several toxic heavy metals are comparable to the commercial luminescent assay, Microtox™ as both clusters together making it a good choice for an alternative near-real-time monitoring of heavy metals. The sensitivity of strain MIE towards heavy metals was proven through field trial works on several heavy metal-polluted sites in Malaysia. Thus, it is a good candidate as an early detection system for heavy metals in aquatic bodies in tropical countries. Springer 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/80742/1/TOXIC.pdf Halmi, Mohd Izuan Effendi and Kassim, Adibah and Abd. Shukor, Mohd. Yunus (2019) Assessment of heavy metal toxicity using a luminescent bacterial test based on Photobacterium sp. strain MIE. Rendiconti Lincei. Scienze Fisiche e Naturali, 30 (3). pp. 358-601. ISSN 1720-0775 https://link.springer.com/article/10.1007/s12210-019-00809-5
institution UPM IR
collection UPM IR
language English
description Toxicity evaluation of wastewater, polluted sediment and water streams is a very crucial aspect of environmental pollution monitoring. In this work, a newly developed luminescent bacterial test using a tropical luminescent bacterium, Photobacterium sp. strain MIE was used to assess the toxicity of several heavy metals using a 15-min assay format. The assessment was carried out by exposing strain MIE to different concentrations of heavy metals ranging from 0.001 to 200 mg/L in a DTX microplate 96 wells. The toxicity result based on the inhibitory concentration (IC50) was Hg (0.053 mg/L) > Ag (0.12 mg/L) > Cu (0.85 mg/L) > Ni (12.32 mg/L) > Zn (18.72 mg/L) > Cr (26.02 mg/L). Principal Component Analysis (PCA) and Agglomerative Hierarchical Clustering (AHC) analyses showed the sensitivity (IC50) of strain MIE to several toxic heavy metals are comparable to the commercial luminescent assay, Microtox™ as both clusters together making it a good choice for an alternative near-real-time monitoring of heavy metals. The sensitivity of strain MIE towards heavy metals was proven through field trial works on several heavy metal-polluted sites in Malaysia. Thus, it is a good candidate as an early detection system for heavy metals in aquatic bodies in tropical countries.
format Article
author Halmi, Mohd Izuan Effendi
Kassim, Adibah
Abd. Shukor, Mohd. Yunus
spellingShingle Halmi, Mohd Izuan Effendi
Kassim, Adibah
Abd. Shukor, Mohd. Yunus
Assessment of heavy metal toxicity using a luminescent bacterial test based on Photobacterium sp. strain MIE
author_facet Halmi, Mohd Izuan Effendi
Kassim, Adibah
Abd. Shukor, Mohd. Yunus
author_sort Halmi, Mohd Izuan Effendi
title Assessment of heavy metal toxicity using a luminescent bacterial test based on Photobacterium sp. strain MIE
title_short Assessment of heavy metal toxicity using a luminescent bacterial test based on Photobacterium sp. strain MIE
title_full Assessment of heavy metal toxicity using a luminescent bacterial test based on Photobacterium sp. strain MIE
title_fullStr Assessment of heavy metal toxicity using a luminescent bacterial test based on Photobacterium sp. strain MIE
title_full_unstemmed Assessment of heavy metal toxicity using a luminescent bacterial test based on Photobacterium sp. strain MIE
title_sort assessment of heavy metal toxicity using a luminescent bacterial test based on photobacterium sp. strain mie
publisher Springer
publishDate 2019
url http://psasir.upm.edu.my/id/eprint/80742/1/TOXIC.pdf
_version_ 1819299686465404928
score 13.4562235

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